Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Mammalian Expression

76,374 results

Showing: 8801 - 8850 of 76374 results

  1. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid
    #228253
    Purpose
    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228253-plasmid-map-sequence-id-451024

    Enlarge

  4. hSyn-eGFP-DDHD2

    Plasmid
    #232463
    Purpose
    Expresses GFP tagged DDHD2 lipase in neurons
    Depositor
    Timothy Ryan
    Insert
    DDHD2 (DDHD2 Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232463-plasmid-map-sequence-id-460234

    Enlarge

  5. mSirt1-GFP

    Plasmid
    #221511
    Purpose
    Fluorescently tagged mouse Sirt1-GFP
    Depositor
    Alessandro Ori
    Insert
    Sirt1 (Sirt1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221511-plasmid-map-sequence-id-439387

    Enlarge

  6. pLNHA-C1-HseIF4G_O

    Plasmid
    #147087
    Purpose
    Mammalian Expression of HseIF4G
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4G (EIF4G1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation M432V compared to the seu…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147087-plasmid-map-sequence-id-289534

    Enlarge

  8. pLNHA-C1-HseIF4AI_K

    Plasmid
    #146731
    Purpose
    Mammalian Expression of HseIF4AI
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4AI (EIF4A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    a non silent mutation (A200V) and 3 silent mutati…
    Promoter
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    146731-plasmid-map-sequence-id-357210

    Enlarge

  12. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

    Enlarge

  13. pCMV-MMLV-gag-hMLH1dn

    Plasmid
    #195513
    Purpose
    MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.
    Depositor
    Hyongbum Kim
    Article
    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.
    Insert
    hMLH1dn (MLH1 Human)
    Use
    Virus like particle
    Tags
    FLAG, NES, and Protease cleavate site
    Expression
    Mammalian
    Mutation
    Human MLH1 del754-756
    Promoter
    CMV
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195513-plasmid-map-sequence-id-384690

    Enlarge

  20. pGW1-mCherry-EGFP-PIM

    Plasmid
    #111759
    Purpose
    High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Article
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    CMV
    Available since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111759-plasmid-map-sequence-id-222507

    Enlarge

  21. TFORF0112

    Plasmid
    #141519
    Purpose
    Lentiviral vector for overexpressing the SMAD2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SMAD2 (SMAD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141519-plasmid-map-sequence-id-276693

    Enlarge

  22. pLentiCRISPR_mCherry

    Plasmid
    #229078
    Purpose
    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1s
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    229078-plasmid-map-sequence-id-456127

    Enlarge

  24. pRK5-myc-Rac1-Q61L

    Plasmid
    #12983
    Depositor
    Gary Bokoch
    Insert
    Rac1 constitutively active (RAC1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q61L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12983-plasmid-map-sequence-id-154551

    Enlarge

  31. TFORF2215

    Plasmid
    #141974
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREB3L4 (CREB3L4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    141974-plasmid-map-sequence-id-395886

    Enlarge

  32. TFORF1509

    Plasmid
    #143511
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT5B (STAT5B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143511-plasmid-map-sequence-id-400402

    Enlarge

  37. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104406-plasmid-map-sequence-id-204465

    Enlarge

  38. pcDNA3.1 acGFP

    Plasmid
    #128047
    Purpose
    Mammalian expression vector with N terminal AcGFP
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    AcGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128047-plasmid-map-sequence-id-255000

    Enlarge

  42. mTagRFP-Membrane-1

    Plasmid
    #57992
    Purpose
    Localization: Plasma Membrane, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    N-terminal 20 Aas MLS from Neuromodulin and mTagR…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    57992-plasmid-map-sequence-id-313444

    Enlarge

  46. pCMV-Flag-hMcl-1

    Plasmid
    #25392
    Depositor
    Roger Davis
    Insert
    MCL1 (MCL1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2010
    Availability
    Academic Institutions and Nonprofits only
    25392-plasmid-map-sequence-id-316915

    Enlarge

  48. pCAG-B19N

    Plasmid
    #59924
    Purpose
    Expression vector for Rabies virus SAD B19 nucleoprotein gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus nucleoprotein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59924-plasmid-map-sequence-id-94984

    Enlarge

  49. pVITRO1-102.1F10-IgM/λ

    Plasmid
    #61880
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61880-plasmid-map-sequence-id-101189

    Enlarge

Showing: 8801 - 8850 of 76374 results