81,052 results

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pCAG PSAM4 GlyR IRES EGFP

Plasmid

#119739

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CAG

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHR GFP-CaaX

Plasmid

#113020

Purpose

for lentivirus production of GFP-CaaX

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

GFP-CaaX

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K11R

Plasmid

#121154

Purpose

Mammalian expression of HA tagged ubiquitin K11R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K11 mutated to an R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMVie-IRES-BlastR

Plasmid

#119863

Purpose

3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

Depositor

Ghassan Mouneimne

Article

Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-EF1Alpha-Tet-on 3G

Plasmid

#118592

Purpose

Expresses Tet-on 3G from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Tet-on 3G

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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tetO.Nfia.Blast

Plasmid

#117270

Purpose

Expresses Nfia and blasticidin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Nfia (Nfia Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tet-on

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.2

Plasmid

#111054

Purpose

Expresses dLight1.2 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry

Plasmid

#108685

Purpose

Strong CAG promoter with mCherry expression for transfection efficacy screening

Depositor

Jordan Green

Article

Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 epsilon

Plasmid

#116886

Purpose

Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 epsilon (YWHAE Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMx-puro-MGT

Plasmid

#111809

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

Insert

Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

viral LTR

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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WT Smarca4-V5 IRES-puro

Plasmid

#107058

Purpose

Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

Depositor

Jerry Crabtree, Courtney Hodges

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

IRES-puroR and V5

Expression

Mammalian

Mutation

wild-type

Promoter

CAG

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

FKBP-EGFP-HOTag3

Expression

Mammalian

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING V155M

Plasmid

#102599

Purpose

Express STING V155M for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

V155M

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRVL-5

Plasmid

#104583

Purpose

Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human IgG1 CH1/CH2/CH3 (IGHG1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-mGCN2-FLAG-IP

Plasmid

#101794

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Gcn2 (Eif2ak4 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-teLuc c-myc

Plasmid

#100026

Purpose

Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

teLuc, a NanoLuc mutant

Tags

c-myc

Expression

Mammalian

Mutation

D19S, D85N, C164H

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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SaBE4

Plasmid

#100805

Purpose

Expresses SaBE4 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

SaBE4

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_SNCA:EGFP

Plasmid

#92195

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-SNCA-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn1-mTurquoise2

Plasmid

#99125

Purpose

An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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mEGFP-PI35P2

Plasmid

#92419

Purpose

Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

Depositor

Geert van den Bogaart

Article

Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

Insert

MCOLN1 (Mcoln1 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Synthetic gene, codon optimized for human.

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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mApple-Sec61b-C1

Plasmid

#90993

Purpose

Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mApple-Sec61b (SEC61B Synthetic, Human)

Tags

mApple (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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piggyBac CMV-GFP-FRT

Plasmid

#92078

Purpose

production of transgenic animal and recombination using flipase

Depositor

Jae Yong Han

Article

Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

Insert

CMV-eGFP and TK-Neo between FRT3 and FRT

Use

Piggybac

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-Blimp1

Plasmid

#73995

Purpose

Plasmid expressing mouse Blimp1

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Blimp1 gene (Prdm1 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-hHMGCR1

Plasmid

#86085

Purpose

expresses the human HMG-CoA reductase isoform 1

Depositor

Anne Galy

Insert

human HMG-CoA Reductase isoform 1 (HMGCR Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only
pDUAL SRBI (GFP)

Plasmid

#86979

Purpose

Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

Depositor

Joe Grove

Insert

SCARB1 (SCARB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV/hPGK

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-LbCpf1-Blast

Plasmid

#84751

Purpose

Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

LbCpf1

Use

CRISPR and Lentiviral

Tags

NLS-3xHA (C terminal on insert)

Expression

Mammalian

Promoter

EFS-NS

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#69138

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE

Tags

none

Expression

Mammalian

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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81,052 results