Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Mammalian Expression

76,423 results

Showing: 11601 - 11650 of 76423 results

  7. TFORF0070

    Plasmid
    #141505
    Purpose
    Lentiviral vector for overexpressing the CSDE1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CSDE1 (CSDE1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    141505-plasmid-map-sequence-id-454897

    Enlarge

  18. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

    Enlarge

  22. pBItetDT12nGFP

    Plasmid
    #80420
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80420-plasmid-map-sequence-id-151410

    Enlarge

  24. TFORF1095

    Plasmid
    #143923
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EN1 (EN1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143923-plasmid-map-sequence-id-391582

    Enlarge

  28. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174644
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Venus-SSPB and iLID-mCherry
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174644-plasmid-map-sequence-id-347042

    Enlarge

  30. PTPRC-bio-His

    Plasmid
    #51813
    Purpose
    Expresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PTPRC (PTPRC Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51813-plasmid-map-sequence-id-79539

    Enlarge

  31. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112669
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112669-plasmid-map-sequence-id-229038

    Enlarge

  33. pDisplay-GRAB_NPY1.0

    Plasmid
    #208675
    Purpose
    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    208675-plasmid-map-sequence-id-411121

    Enlarge

  34. pMA2780 lenti tet on EGFP

    Plasmid
    #128043
    Purpose
    Tet inducible Lentiviral vector with EGFP co-expression
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128043-plasmid-map-sequence-id-254872

    Enlarge

  39. pMIG-w

    Plasmid
    #12282
    Purpose
    a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus
    Depositor
    Luk Parijs
    Article
    Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  43. pMP597

    Plasmid
    #134999
    Purpose
    Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134999-plasmid-map-sequence-id-267926

    Enlarge

Showing: 11601 - 11650 of 76423 results