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82,463 results

Showing: 521 - 540 of 82463 results

  2. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    28 Articles
    60229-plasmid-map-sequence-id-419029

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  13. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    24 Articles
    73797-plasmid-map-sequence-id-196924

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  19. pSicoR

    Plasmid
    #11579
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Tyler Jacks
    Publication
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74 Articles
    11579-plasmid-map-sequence-id-251845

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  20. pCDNA3.1(+)-CMV-bArrestin2-TEV

    Plasmid
    #107245
    Purpose
    Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ARRB2
    Tags
    TEV(NIA), tobacco etch virus NIA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    107245-plasmid-map-sequence-id-322368

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Showing: 521 - 540 of 82463 results