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81,075 results

Showing: 11521 - 11550 of 81075 results

  1. pJC49

    Plasmid
    #133060
    Purpose
    Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.
    Depositor
    Martin Kampmann
    Article
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    Tau.K18(LM)
    Use
    Lentiviral
    Tags
    mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133060-plasmid-map-sequence-id-271174

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  4. pAF112

    Plasmid
    #135920
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Luciferase; Transposon
    Expression
    Mammalian
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135920-plasmid-map-sequence-id-270078

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  7. pTRE3G-Bi-gePSI-pCMV-AcGFP

    Plasmid
    #131008
    Purpose
    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.
    Depositor
    Erin Schuman
    Article
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Inserts
    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    Use
    AAV
    Tags
    murine ornithine decarboxylase - degron (ODC36)
    Expression
    Mammalian
    Promoter
    pCMV and pTRE3G-Bi
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131008-plasmid-map-sequence-id-266807

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  14. pLenti-rInsp-hIns-GLuc

    Plasmid
    #89927
    Purpose
    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    410bp rat insulin II promoter
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    89927-plasmid-map-sequence-id-255126

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  16. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid
    #123235
    Purpose
    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available Since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123235-plasmid-map-sequence-id-378779

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  23. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available Since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  28. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    117699-plasmid-map-sequence-id-368109

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  30. mito-mGarnet2

    Plasmid
    #104310
    Purpose
    codes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito
    Depositor
    Ulrich Nienhaus
    Article
    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97
    Insert
    mito-mGarnet2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104310-plasmid-map-sequence-id-225636

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Showing: 11521 - 11550 of 81075 results