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Plasmid#65830PurposeFor membrane contactDepositorInsertHomo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
UseTagsspGFP11ExpressionWormMutationPromoterPrig-3Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHW530 (Prab-3::cGAL-N::let-858 3'UTR)
Plasmid#107131PurposecGAL-N driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3196
Plasmid#172696PurposePrgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)DepositorInsertPrgef-1
UseTagsGFP 1-10ExpressionWormMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD49_78
Plasmid#1447DepositorTypeEmpty backboneUseTagshsp16-415', hsp16-25', IVSA, rf2128ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGLOW77
Plasmid#177338PurposeC. elegans mNeonGreen co-injection markerDepositorInsertPmyo-2::mNeonGreen
UseTagsmNeonGreenExpressionWormMutationPromotermyo-2Available sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1219
Plasmid#61250PurposeCRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegansDepositorInsertsgRNA(F+E)
UseCRISPRTagsExpressionWormMutationPromoterU6 promoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMLS134
Plasmid#73714PurposeSapTrap destination vector for building sgRNA expression vectors.DepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDD317 (Pmyo-2::GFP + SEC)
Plasmid#73344PurposeSEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology armsDepositorInsertPmyo-2::GFP + SEC
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromotermyo-2Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
BSP605
Plasmid#122250Purposeexpression vector for mCerulean under EFT3 promoterDepositorInsertmCerulean
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]
Plasmid#168173PurposeExpression of BeCyclOp in BWMs of C. elegansDepositorInsertBeCyclOp (codon optimized for C. elegans)
UseTagsExpressionWormMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1-gpa-2P::his-24::Cerulean
Plasmid#124337PurposeMulticolor Worms for Neuronal IDDepositorInsertgpa-2, Cerulean (gpa-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Prab-3::NLS::GCaMP6s
Plasmid#68119PurposeNuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. ElegansDepositorInsertrab-3::NLS::GCaMP6s
UseTagsExpressionWormMutationPromoterrab-3Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS1-lim-4P::his-24::Cerulean
Plasmid#124336PurposeMulticolor Worms for Neuronal IDDepositorInsertlim-4, Cerulean (lim-4 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
Plasmid#159803PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-GFP(dpiRNA)::CDK-1
Plasmid#107938Purposecdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertGFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
UseTagsExpressionWormMutationPromotercdk-1Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
TetR in pENTR1A (559-1)
Plasmid#22265DepositorInsertTetracycline repressor (tetR )
UseTagsExpressionInsect, Mammalian, Worm Exp…MutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXF88
Plasmid#139039PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInserthalotag (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGM32DEST
Plasmid#74747PurposeEncodes for the QF-GR recombinant gene, splice linker, and mCherry reporter geneDepositorInsertsUseTagsFusion of the Neurospora crassa QF activator to t…ExpressionWormMutationPromoterNo promoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
TC358
Plasmid#122613PurposeTRE-delta-pes-10-GFPDepositorInsert7XTRE-delta-pes-10-GFP
UseTagsExpressionWormMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)
Plasmid#107130PurposecGAL-C driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD283
Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLS716
Plasmid#188888PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::CreDepositorInsertfloxed Cbr-unc-119 marker and Phsp16.41::Cre
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wElectra1
Plasmid#184932PurposeCodon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmidDepositorInsertElectra1
UseTagsExpressionWormMutationPromotertag-168Available sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP713-T2A-HO1
Plasmid#197246PurposeExpresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegansDepositorInsertwmiRFP713-T2A-HO1
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM3 = ncs-1p::nCre
Plasmid#24034DepositorInsertncs-1 (ncs-1 Nematode)
UseCre/LoxTagsnCreExpressionWormMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBN154
Plasmid#86718PurposeFLP/Frt dual color reporter for C. elegansDepositorInsertPhsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
UseTagsExpressionWormMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionWorm and YeastMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
BSP610
Plasmid#122255Purposeexpression vector for mtagBFP2 under EFT3 promoterDepositorInsertmtagBFP2
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)
Plasmid#47550PurposeCas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion alleleDepositorInsertsCas9
ttTi5605 sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-set2
Plasmid#82546PurposePmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::set-2 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TC374
Plasmid#122614PurposePrpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
UseTagsExpressionWormMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLT61
Plasmid#1690DepositorTypeEmpty backboneUseRNAiTagsT7p, unc224616, unc2218148, unc2218082, unc223827…ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJW1586
Plasmid#121057PurposemKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmKate2-T^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized mKate2, and au…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2
Plasmid#182357PurposePositive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.DepositorInsertGFP
UseTagsExpressionWormMutationPromoterPeft-3Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmec-4::Chrimson::SL2::mCherry::unc-54
Plasmid#107745PurposeExpress Chrimson and mCherry in mechanosensory neurons.DepositorInsertsChrimson
mCherry
UseTagsExpressionInsect and WormMutationPromotermec-4Available sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHT101-mCherry
Plasmid#61021PurposeExpresses mCherry tagged fusion proteins in wormsDepositorTypeEmpty backboneUseTagsmCherryExpressionWormMutationPromoterlacAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNM3814
Plasmid#154122Purposemec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vectorDepositorInsertmec-4p::rtetR-L-QFAD::act-4 '3
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM-GFPnovo2
Plasmid#116943PurposeGFPnovo2_unc-54 utr vector for expression in C. elegansDepositorInsertC. elegans codon optimized GFPnovo2
UseTagsGFPnovo2ExpressionWormMutationY145F, V163A, S202T, L221VPromoternoneAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1285
Plasmid#61252PurposeCRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegansDepositorInsertsgRNA(F+E) targeting pha-1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEM246 - MCS mosTI unc-119 target
Plasmid#159821PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertMCS mosTI unc-119 target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
Plasmid#159849PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2
Plasmid#182358PurposePositive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.DepositorInsertGFP
UseTagsExpressionWormMutationPromoterPmex-5Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD121
Plasmid#91833PurposeCas9 expression in C. elegansDepositorInsertCas9
UseCRISPRTagsExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR49
Plasmid#69259Purposephlh-8::tagBFP2DepositorInsertTagBFP2
UseCre/LoxTagsExpressionWormMutationcodon-optimzed index 1.0, TagBFP2 contains 2 sing…Promoterhlh-8Available sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNM3724
Plasmid#154112PurposetetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vectorDepositorInserttetO 7X::∆pes-10::GFP-C1::tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159857PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLGCV
Plasmid#49862PurposeExpression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).DepositorInsertfirefly luciferase luc+ fused to GFP (S65C)
UseTagsGFP (S65C)ExpressionWormMutationPromotersur-5Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)
Plasmid#193853PurposePromoterless insertion vector for split HygR/mScarlet-I landing padDepositorInsert5' rps-0::HygR + 5' mScarlet-I
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterrps-0pAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only