Skip to main content
Addgene
Showing: 451 - 500 of 7933 results

  3. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

    Enlarge

  6. pT040

    Plasmid
    #67640
    Purpose
    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Insert
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67640-plasmid-map-sequence-id-120917

    Enlarge

  8. pBHM2403

    Plasmid
    #171687
    Purpose
    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    CnoCAS9
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171687-plasmid-map-sequence-id-340174

    Enlarge

  10. pRS420

    Plasmid
    #11259
    Depositor
    Fred Cross
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11259-plasmid-map-sequence-id-6933

    Enlarge

  27. pGR618

    Plasmid
    #213786
    Purpose
    Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BB-Tv TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213786-plasmid-map-sequence-id-424624

    Enlarge

  33. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    104907-plasmid-map-sequence-id-206042

    Enlarge

  35. pCdOpt-BMX

    Plasmid
    #203929
    Purpose
    This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae
    Depositor
    Scott Briggs
    Article
    Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11
    Insert
    BleMX
    Use
    Tags
    Expression
    Yeast
    Mutation
    BleMX was codon optimized for Candida albicans or…
    Promoter
    Available since
    Dec. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    203929-plasmid-map-sequence-id-414816

    Enlarge

  38. pICE ADE2

    Plasmid
    #122876
    Purpose
    ADE2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.
    Depositor
    Randy Hampton
    Article
    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340
    Type
    Empty backbone
    Use
    Yeast recombination cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122876-plasmid-map-sequence-id-238615

    Enlarge

  40. pCfB2223

    Plasmid
    #67544
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67544-plasmid-map-sequence-id-116393

    Enlarge

  43. pRS143

    Plasmid
    #122377
    Purpose
    CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122377-plasmid-map-sequence-id-241241

    Enlarge

  44. UASGAL-Pgal

    Plasmid
    #60338
    Purpose
    Contains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60338-plasmid-map-sequence-id-96619

    Enlarge

Showing: 451 - 500 of 7933 results