Skip to main content
Addgene
Showing: 551 - 600 of 7933 results

  6. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

    Enlarge

  7. pBHM2642

    Plasmid
    #229526
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229526-plasmid-map-sequence-id-459924

    Enlarge

  8. pBHM2644

    Plasmid
    #229528
    Purpose
    Carries AID* tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NATR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229528-plasmid-map-sequence-id-459927

    Enlarge

  9. pBHM2645

    Plasmid
    #229529
    Purpose
    Carries mIAA7_opt tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    mIAA7_opt - empty sgRNA - NATR
    Use
    CRISPR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229529-plasmid-map-sequence-id-459936

    Enlarge

  10. pBHM2646

    Plasmid
    #229530
    Purpose
    Carries mAID_opt tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    mAID_opt - empty sgRNA - NATR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229530-plasmid-map-sequence-id-459934

    Enlarge

  11. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

    Enlarge

  12. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

    Enlarge

  13. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

    Enlarge

  14. pBHM2650

    Plasmid
    #229992
    Purpose
    Carries AID* tag, empty sgRNA, and HYGR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - HYGR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229992-plasmid-map-sequence-id-461779

    Enlarge

  15. pBHM2651

    Plasmid
    #229993
    Purpose
    Carries AID* tag, empty sgRNA, and NEOR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NEOR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229993-plasmid-map-sequence-id-461132

    Enlarge

  16. pBHM2652

    Plasmid
    #229994
    Purpose
    Carries AID* tag, empty sgRNA, and amdS marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - amdS
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229994-plasmid-map-sequence-id-461120

    Enlarge

  17. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

    Enlarge

  18. pRS416-yZ3EV-Z3pr (RB3578)

    Plasmid
    #69099
    Purpose
    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr
    Depositor
    David Botstein
    Inserts
    yZ3EV cassette
    Z3 promoter
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69099-plasmid-map-sequence-id-122355

    Enlarge

  19. ADH1pro_GAL4_SIR4

    Plasmid
    #138621
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GAL4 (GAL4 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    138621-plasmid-map-sequence-id-334953

    Enlarge

  20. ZJOM75

    Plasmid
    #133649
    Purpose
    Integration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-ANP1-mCherry (ANP1 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133649-plasmid-map-sequence-id-267445

    Enlarge

  22. p406CYC1

    Plasmid
    #15975
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    CYC1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15975-plasmid-map-sequence-id-8460

    Enlarge

  27. pDIV515

    Plasmid
    #177703
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177703-plasmid-map-sequence-id-353317

    Enlarge

  30. pREP41-MCS+

    Plasmid
    #52690
    Purpose
    Same as pREP41, with better MCS
    Depositor
    Michael Nick Boddy
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pnmt41
    Available since
    April 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    52690-plasmid-map-sequence-id-82000

    Enlarge

  33. pJT1

    Plasmid
    #98299
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    98299-plasmid-map-sequence-id-189966

    Enlarge

  40. pESC-NAT-LACap

    Plasmid
    #80763
    Purpose
    Expresses truncated L-A capsid for curing satellite virus
    Depositor
    John McCusker
    Insert
    L-A cDNA (L-A Cap)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80763-plasmid-map-sequence-id-156222

    Enlarge

  42. pPS3858

    Plasmid
    #113393
    Purpose
    galactose-inducible Ty3-his3AI; additional PPT
    Depositor
    Suzanne Sandmeyer
    Insert
    Ty3-his3AI
    Use
    Tags
    his3AI
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113393-plasmid-map-sequence-id-218651

    Enlarge

  50. ZJOM43

    Plasmid
    #133652
    Purpose
    Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPS4-DuDre (VPS4 Budding Yeast)
    Use
    Tags
    DuDre
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133652-plasmid-map-sequence-id-308131

    Enlarge

Showing: 551 - 600 of 7933 results