8,265 results
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Plasmid#84608PurposeIntroduces DSB at AXP locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-EGFP-ccdB
Plasmid#14323DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRG203MX
Plasmid#64521PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-ฮ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIB1
Plasmid#28245PurposeExpresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activationDepositorAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMZ379
Plasmid#74215PurposeCombined sgRNA/Cas9 vector with NatMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
RPL25-GFP (94)
Plasmid#24037DepositorAvailable SinceMarch 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS327
Plasmid#51787Purposemulticopy (YEp) vector with a LYS2 marker and a 2-ฮผm origin of replicationDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
IQ-compete reporter (integrating)
Plasmid#247176PurposePlasmid for genomic integration of the IQ-compete reporter (GFP-TCS-Quencher-CL1) into the YPRCฮ15 locus of yeast cells.DepositorInsertGFP-TCS-Quencher-CL1
ExpressionYeastPromoterTEF1Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
DHFR-TEV (integrating)
Plasmid#247218PurposePlasmid for genomic integration of DHFR-TEV into the YORWฮ22 locus of yeast cells.DepositorInsertDHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Atp5-DHFR-TEV (integrating)
Plasmid#247219PurposePlasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWฮ22 locus of yeast cells.DepositorInsertAtp5 MTS-DHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Oxa1-DHFR-TEV (integrating)
Plasmid#247220PurposePlasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWฮ22 locus of yeast cells.DepositorInsertOxa1 MTS-DHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWJ 1781 URA v-Src FL
Plasmid#245255PurposeExpression of full-length viral Src in yeastDepositorInsertFull-length viral Src
ExpressionYeastMutationwild-typeAvailable SinceSept. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWJ 1781 URA
Plasmid#245252PurposeProtein expression in yeastDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pYES2-Ub-G76V-GFP
Plasmid#11954DepositorInsertUb-G76V-GFP
ExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Glycine 76 โฆAvailable SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pV1393
Plasmid#111430PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETcon_SARS-CoV-2_RBD
Plasmid#166782PurposeYeast surface display vector, expressing the SARS-2 RBD, 201aa constructDepositorInsertSpike receptor binding domain (RBD) from SARS-CoV-2 (S )
ExpressionYeastAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
TagsGFPExpressionYeastAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRExpressionYeastAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CRU313
Plasmid#131163PurposeFor ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneTagsCRUExpressionYeastAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDP0437
Plasmid#221127PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMXDepositorInsertpHluorin
ExpressionYeastAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-T7-GFP
Plasmid#33130DepositorAvailable SinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMZ374
Plasmid#59896PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-V5-hphMX4
Plasmid#20779DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
yHS1-M7A
Plasmid#159151PurposeCytosolic labile heme reporterDepositorInsertyHS1-M7A
ExpressionYeastMutationMutated Met 7 of the cyt b562 module of yHS1 to AโฆPromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-6xGLY-FLAG-kanMX6
Plasmid#20751DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
CPY-ymsfGFP
Plasmid#209538PurposeExpresses CYP tagged with ymsfGFP in S. cerevisiaeDepositorInsertCPY
TagsymsfGFPExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPY*-ymsfGFP
Plasmid#209539PurposeExpresses the misfolded CPY* mutant tagged with ymsfGFP in S. cerevisiaeDepositorInsertCPY*
TagsymsfGFPExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
Plasmid#198417PurposeExpresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::HIS3
Plasmid#227596PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::HIS3
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
Plasmid#58442PurposeCassette to replace yeast promoters with an inducible promoter containing 4 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
ExpressionYeastPromoterPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
YEp181-CUP1-His-Ubi
Plasmid#99539PurposeYeast episomal vector for expression of His-tagged ubiquitin; LEU2 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2793
Plasmid#64043PurposepCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoter and noneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPM28 (eroGFP CEN/ARS URA3)
Plasmid#20131DepositorInserteroGFP (KAR2 Budding Yeast, jellyfish)
ExpressionYeastAvailable SinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPS22B-Cl
Plasmid#160431PurposeExpresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoterDepositorInsertRPS22B (RPS22B Budding Yeast)
TagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to change nucleoโฆPromoterGAL1 and S. cerevisiae GAL1Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIp204-PADH1-atTIR1-9myc
Plasmid#99532PurposeS. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
RPL3-GFP (96)
Plasmid#24040DepositorAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pN3
Plasmid#233520PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and HygroR (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYZ960
Plasmid#187972PurposeTEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2ยต-URA3DepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA CUP1p-NuiHA kanMX
Plasmid#131169PurposePCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneTagsNui-HAExpressionYeastAvailable SinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS552
Plasmid#242852PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceSept. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymTurquoise2-Kan
Plasmid#224092PurposeYeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selectionDepositorInsertymTurquoise2
TagsymTurquoise2ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
ymScarlet
Plasmid#111917Purposeyeast optimized mScarletDepositorInsertymScarlet
ExpressionYeastPromoterGPDAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM149
Plasmid#133646PurposeIntegration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TKC-DsRed-Express2-HDEL
Plasmid#21770DepositorInsertDsRed-Express2
TagsHDEL and preKar2ExpressionYeastAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
RPS1B-GFP (95)
Plasmid#24039DepositorAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKanB
Plasmid#223523PurposeUsed for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable markerDepositorTypeEmpty backboneExpressionYeastPromoterAOX1Available SinceSept. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits