8,350 results
-
Plasmid#84611PurposeIntroduces DSB at D17 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXNubA22-Dest
Plasmid#105087PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneTagsNubAExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
TetR in pENTR1A (559-1)
Plasmid#22265DepositorInsertTetracycline repressor (tetR )
ExpressionInsect, Mammalian, Worm Exp…Available SinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
HSP104-b/Leu(WT)
Plasmid#1156DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pVS1
Plasmid#33143DepositorInsertLacO (256 copies)
ExpressionYeastAvailable SinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIGAhRC
Plasmid#112510Purposehuman Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoterDepositorUseSynthetic BiologyExpressionYeastPromoterGal1,10Available SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-GAL1
Plasmid#85782PurposeN-ICE plasmid pH3FS-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pFA6a-GFP11-His3MX
Plasmid#91951PurposeUniversal C-terminal GFP11 taggingDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNXgate33-3HA
Plasmid#105075PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneTags3xHA and NubGExpressionYeastAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#160389PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selectionDepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseCen/arsExpressionYeastPromoterEndogenous ATG8 promoterAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS303H-Padh-OsTIR1(F74G)
Plasmid#247625PurposeExpression of OsTIR1DepositorInsertOsTIR1
ExpressionYeastMutationF74GPromoterADH1Available SinceJan. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-GAL1
Plasmid#85778PurposeN-ICE plasmid pK3FS-GAL1DepositorInsertloxP-KanMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-Kan
Plasmid#224077PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selectionDepositorInsertymScarletI3
TagsymScarletI3ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_MFE1
Plasmid#84612PurposeIntroduces DSB at MFE1 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet14
Plasmid#107451PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM319
Plasmid#84201PurposeDisturb the expression of miR319 in riceDepositorInsertmiR319
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH727-CEN-RLuc/staCFLuc
Plasmid#38211DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYZ463
Plasmid#187971PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
JME4599
Plasmid#129660PurposeNATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterENO1pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT0127
Plasmid#8728DepositorInsertyEGFP
TagsCodon optimized linkerExpressionYeastAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
pDS244
Plasmid#34982DepositorAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mCherry
Plasmid#125700PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mCherryDepositorInsertymNeongreen-T2A-mCherry
ExpressionYeastAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pHM4 (U6 in pSE360)
Plasmid#111281PurposeU6 snRNA coding sequence in pSE360(Ura) plasmidDepositorInsertSNR6
ExpressionYeastAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C1
Plasmid#125549PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to c-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C2
Plasmid#125559PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to c-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-GR100
Plasmid#84908Purposeinduce expression of dipeptide repeat GR in yeastDepositorAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N2
Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB
Plasmid#14148DepositorTypeEmpty backboneUseGateway CloningExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HPH-C4
Plasmid#160343PurposeHigh-copy expression vector for K. marxianus, hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM153
Plasmid#133642PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPTK002-2-pGAP
Plasmid#84961PurposeType 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoterDepositorInsertGlyceraldehyde-3-phosphate dehydrogenase promoter
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1805 c to g)PromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES-mtGFP
Plasmid#45053DepositorInsertmtGFP
ExpressionYeastPromoterGALAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pABY-T2
Plasmid#201765PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
ExpressionYeastAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-CLIP-KanMX6
Plasmid#87025PurposeTag S. pombe genes in C terminal with CLIPDepositorInsertCLIP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS327
Plasmid#51787Purposemulticopy (YEp) vector with a LYS2 marker and a 2-μm origin of replicationDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB-EGFP
Plasmid#14203DepositorTypeEmpty backboneUseGateway CloningTagsEGFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYX233-DHFR
Plasmid#163761PurposeGal-inducible yeast expression of cytosolic DHFRDepositorAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPTK003-2-pENO1
Plasmid#84962PurposeType 2, pENO1, Enolase 1 promoterDepositorInsertEnolase 1 promoter
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (2350 g to c) and BsmBI site re…PromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWS3865
Plasmid#185742PurposeSub-array backbone 1/4DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEOS3.2-KanMX6
Plasmid#87030PurposeTag S. pombe genes in C terminal with mEOS3.2DepositorInsertmEOS3.2
ExpressionBacterial and YeastAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr (RB3578)
Plasmid#69099PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3prDepositorInsertsyZ3EV cassette
Z3 promoter
ExpressionYeastAvailable SinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRExpressionYeastPromoterpRPR1(TetO)Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p405-BrdU-Inc
Plasmid#71791PurposeBrdU-incorporating plasmid with selectable marker LEU2DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETcon_SARS-CoV-2_RBD
Plasmid#166782PurposeYeast surface display vector, expressing the SARS-2 RBD, 201aa constructDepositorInsertSpike receptor binding domain (RBD) from SARS-CoV-2 (S )
ExpressionYeastAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIB1
Plasmid#28245PurposeExpresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activationDepositorAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only