8,173 results
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Plasmid#14321DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only
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PhyB-mCherry-Htb2
Plasmid#51569PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsHTB2 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-CaURA3
Plasmid#44887PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with CaUra3 selectionDepositorInsertyomEos2
ExpressionYeastAvailable SinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-PRUNE-HA
Plasmid#183945PurposeExpresses human HA-tagged PRUNE from yeast GPD promoterDepositorAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIP2
Plasmid#31436DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUDP012
Plasmid#101167PurposeE. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL_yEGFP_hc
Plasmid#98746PurposeReporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.DepositorInsertYeast-enhanced green fluorescent protein
UseSynthetic BiologyExpressionYeastPromotersyntheticAvailable SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-pol30(K164R)
Plasmid#99544PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2-eGFP-StII-6xHis
Plasmid#124277PurposeExpresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G11-5FLAG-kanMX6
Plasmid#49182Purposegenomic G11-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneTagsG11-5FLAGExpressionYeastAvailable SinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGPD-luxAB(HIS)
Plasmid#1106DepositorInsertbacterial luciferase
ExpressionYeastMutationn/aAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKT0140
Plasmid#8732DepositorInsertyECitrine
TagsCodon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203YAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
G31, Cdc20-AID tagging
Plasmid#130269PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombinationDepositorInsertCdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
TagsAID(71-114)ExpressionYeastPromoterNative promoterAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-6xGLY-Myc-HIS3MX6
Plasmid#20768DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-SpHis5
Plasmid#44847PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selectionDepositorInsertyomKeima
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFTK036
Plasmid#171308PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertamdS ANIA_08777 acetamidase, selection marker
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSK267
Plasmid#129272Purpose"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed proteinDepositorInsertNLS-VP16-ZF 43-8-NES
Tags6xHAExpressionYeastPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaARG4
Plasmid#162463PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4DepositorInsert3FLAG-Mnase
ExpressionYeastAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMH120
Plasmid#40593PurposeExpresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expressionDepositorAvailable SinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomWasabi-Kan
Plasmid#44945PurposeYeast tagging vector to create gene fusion with yeast optimized mWasabi with Kan selectionDepositorInsertyomWasabi
ExpressionYeastAvailable SinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB-EGFP
Plasmid#14181DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6L1TAG
Plasmid#158148PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertAga1p-FAPB2.3.6
ExpressionYeastMutationCloned in a TAG codon at the first position of th…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Erg11-iGFPx3
Plasmid#115422PurposeExpress Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertErg11-iGFPx3
TagsiGFPExpressionYeastPromoterEndogenousAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK087
Plasmid#171359PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPS1372
Plasmid#1353DepositorInsertpADH-NLS-NES-GFP2
ExpressionYeastAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDS1209
Plasmid#177340PurposeExpresses Aequorin in Candida albicans using the URA3 auxotrophic marker URA3DepositorInsertAequorin
ExpressionYeastPromoterACT1Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GAL 72Q+ProGFPp416
Plasmid#15582DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-Kan
Plasmid#44958PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with Kan selectionDepositorInsertyomKO2
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
426Gal-FUS-RRM Mutant-YFP
Plasmid#29608DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTET
Plasmid#46923PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoterDepositorInsertsgRNA targeting endogenous TRE elements of pTET07 promoter
UseCRISPRExpressionYeastPromoterSNR52Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-GGA2-msGFPx3
Plasmid#105258PurposePlasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT11F
Plasmid#165062PurposeIntroducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
ExpressionYeastMutationWTAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLUC
Plasmid#1280DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-luxTA2-4F
Plasmid#166030PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-phlTA2-1E
Plasmid#166029PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)
Plasmid#179054PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubulesDepositorInsertRPL18Bp-Scarlet(rfp)-Link-TUB1
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG
Plasmid#176400PurposeExpresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.DepositorInsertFRB-RGG-GFP-RGG-RGG
TagsFRB and GFPExpressionYeastPromoterGAL1Available SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-X4
Plasmid#176154Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XII1
Plasmid#176159Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP43
Plasmid#65519PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI3
Plasmid#176157Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP39
Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mTurquoise2
Plasmid#125701PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2DepositorInsertymNeongreen-T2A-mTurquoise
ExpressionYeastAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSX6
Plasmid#90468PurposeU6 wt in pSE358 (TRP1 CEN)DepositorAvailable SinceJune 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPS811
Plasmid#8904DepositorAvailable SinceAug. 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPGP_scFv
Plasmid#183732PurposeExpresses anti-lysozyme scFv antibodyDepositorInsertAnti-lysozyme scFv
TagsHis6-tagExpressionYeastPromoterGAP promoterAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV113
Plasmid#63180PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only