We narrowed to 5,620 results for: SUP

1. pCI pHluorin Syt7

   Plasmid

   #184508

   Purpose

   Mammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 7 (Syt7 Mouse)
   

   Use

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAS1NB c Rosella I

   Plasmid

   #71245

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-His6-GFP-C-LACE

   Plasmid

   #133913

   Purpose

   Bacterial expression of His6-GFP-C-LACE

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpyTag-sfGFP

   Plasmid

   #184226

   Purpose

   Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpyTag-sfGFP
   

   Use

   Tags

   His6, SpyTag, and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSR59.4

   Plasmid

   #63175

   Purpose

   Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   OmpR (ompR )
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-6xHis-GFP(+30)

   Plasmid

   #199164

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+30)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-VHL wt-pBabe-puro

   Plasmid

   #19234

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50455

   Purpose

   Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV8

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTriEx-mCherry-LOV2 V416L

   Plasmid

   #81036

   Purpose

   mammalian expression of LOV V416L, a mutant with super long relaxation time

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV V416L
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   V416L

   Promoter

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDule-Tet3.0

    Plasmid

    #174080

    Purpose

    Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    Tet3.0 tRNA synthetase and tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L270G, Y271L, N311G, C313A

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. single cut NH-donor

    Plasmid

    #83575

    Purpose

    eGFP reporter donor containing single SA cutting site for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 4xHRE_YB TATA-sfGFP-CMV_dsRed

    Plasmid

    #117399

    Purpose

    sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. double cut NH-donor

    Plasmid

    #83576

    Purpose

    eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET15b T7pCONS TIR-2 sfGFP

    Plasmid

    #154468

    Purpose

    pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCI pHluorin Syt2

    Plasmid

    #184503

    Purpose

    Mammalian expression of Synaptotagmin 2 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 2 (Syt2 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS-puro shNEDD4L #1

    Plasmid

    #27016

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    shRNA NEDD4L #1 (NEDD4L Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGAN230

    Plasmid

    #131122

    Purpose

    reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTriEx-mCherry-LOV2 I427T

    Plasmid

    #81035

    Purpose

    Mammalian expression of LOV I427T, a mutant with super short relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427T
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427T

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mNeonGreen-Smad3

    Plasmid

    #172098

    Purpose

    Expresses fluorescently tagged human Smad3 in mammalian cells.

    Depositor

    Lea Goentoro

    Article

    Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Smad 3 (Smad3 Mouse)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    C49S mutation in mCerulean

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 4xHRE_minCMV-sfGFP-CMV_dsRed Exp

    Plasmid

    #117401

    Purpose

    sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO.1P shACO1

    Plasmid

    #169884

    Purpose

    Suppress ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    shRNA against ACO1
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCI pHluorin Syt10

    Plasmid

    #184511

    Purpose

    Mammalian expression of Synaptotagmin 10 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 10 (Syt10 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50456

    Purpose

    Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXZX501

    Plasmid

    #169455

    Purpose

    pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATa
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXZX351

    Plasmid

    #169454

    Purpose

    pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATα
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET15b T7pCONS sfGFP

    Plasmid

    #154467

    Purpose

    pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCI pHluorin Syt4

    Plasmid

    #184505

    Purpose

    Mammalian expression of Synaptotagmin 4 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 4 (Syt4 Rat)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 2a-copGFP(+HA)

    Plasmid

    #83806

    Purpose

    Express copGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    copGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTriEx-mCherry-LOVSD

    Plasmid

    #81033

    Purpose

    Mammalian expression of the super-dark state of LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOVSD
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    C450A, L514K, G528A, L531E, N538E

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHS-393

    Plasmid

    #195473

    Purpose

    Lentiviral Integratable Inducible super-IL2 cytokine + synZiFTR10

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF10 BS - ybTATA - IL12] - pSFFV - ZF10 - NS3 - p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCB11

    Plasmid

    #201111

    Purpose

    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

    Depositor

    Mary Dunlop

    Article

    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

    Inserts

    CcaR
    tetA(C)
    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Promoter

    J23100 and PcpcG2-172

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCI pHluorin Syt5

    Plasmid

    #184506

    Purpose

    Mammalian expression of Synaptotagmin 5 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 5 (Syt5 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET30 GLTP

    Plasmid

    #170733

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGAN147

    Plasmid

    #129202

    Purpose

    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLKO.1P shTFRC

    Plasmid

    #169882

    Purpose

    Suppress TRFC

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    shRNA against TFRC
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-6xHis-GFP(+24)

    Plasmid

    #199163

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+24)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKar2-msGFP-HDEL

    Plasmid

    #117668

    Purpose

    Localisation of Endoplasmic Reticulum

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pKar2-msGFP-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains the Kar2 promoter and signal sequence fr…

    Promoter

    pKar2

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRS-puro shNEDD4L #2

    Plasmid

    #27017

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    shRNA NEDD4L #2 (NEDD4L Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

42. p2xFlag CMV2 Lats1-PY1 mutant

    Plasmid

    #18973

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Large tumor suppressor kinase 1 PY-1 mutant (LATS1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    PY1 mutant, Tyrosine 376 changed to Alanine

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

43. pET28a-ybbR-HIS-sfGFP-DocI

    Plasmid

    #58708

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    sfGFP-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. 4xHRE_minSV40-sfGFP-CMV_dsRed

    Plasmid

    #117400

    Purpose

    sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. pSTARR-seq_fly-RpS12

    Plasmid

    #71504

    Purpose

    flySTARR-seq screening vector with RpS12 core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSR-puro-Exo84

    Plasmid

    #31116

    Depositor

    Christopher Counter

    Article

    Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

    Insert

    Exo84 shRNA (EXOC8 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

    Plasmid

    #162804

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. MSCV KSR1 IRES GFP

    Plasmid

    #25973

    Depositor

    Rob Lewis

    Article

    Kortum et al Mol Cell Biol. 2004 May . 24(10):4407-16

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

49. pEVOL-MmAcKRS1-PylTUUA

    Plasmid

    #182537

    Purpose

    N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppression

    Depositor

    Wenshe Liu

    Article

    Wu et al Chembiochem. 2012 Jul 9;13(10):1405-8. d

    Insert

    PylTUUA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProK

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. prCAG-RBS"m1"-sfGFP

    Plasmid

    #191400

    Purpose

    Expression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)

    Depositor

    Ariel Lindner

    Article

    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

    Insert

    47xCAG RBS"m1"-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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