4,132 results

1. EF.hICN1.CMV.GFP

   Plasmid

   #17623

   Purpose

   Depositor

   Linzhao Cheng

   Article

   Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

   Insert

   ICN1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFast-FT-N1

   Plasmid

   #31910

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   CMV

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag pLJM1 RagB wt

   Plasmid

   #19313

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding B (RRAGB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMT3 p38

   Plasmid

   #12658

   Depositor

   John Kyriakis

   Insert

   p38alpha (MAPK14 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-Myc-ASC

   Plasmid

   #73952

   Purpose

   Express c-Myc-tagged human ASC in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

   Insert

   PYD and CARD domain containing, PYCARD (PYCARD Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMSCV-IRES-DsRed FP (pMIDsRed II)

   Plasmid

   #52110

   Purpose

   a bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-DsRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hATMS1981A

   Plasmid

   #32300

   Depositor

   Michael Kastan

   Article

   Bakkenist et al Nature. 2003 Jan 30;421(6922):499-506.

   Insert

   ATM (ATM Human)
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   S1981A

   Promoter

   Available since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-HA-UCHL1

   Plasmid

   #22563

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   UCHL1 (UCHL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV Myc T58A puro

   Plasmid

   #18773

   Depositor

   Scott Lowe

   Article

   Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

   Insert

   myc T58A (MYC Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T58A

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV CD68-hM4D(Gi)-mCherry

    Plasmid

    #75033

    Purpose

    Expression of hM4D(Gi) using the CD68 promoter.

    Depositor

    Bryan Roth

    Article

    Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Has Service

    AAV1 and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CD68

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKH3

    Plasmid

    #12555

    Depositor

    Ian Macara

    Article

    Mattingly et al Nature. 1996 Jul 18. 382(6588):268-72.

    Type

    Empty backbone

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET30a-AlkB

    Plasmid

    #79050

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe-puro Ras V12

    Plasmid

    #1768

    Depositor

    Bob Weinberg

    Insert

    ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V, constitutively active

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1 puro shRNA beta-catenin

    Plasmid

    #18803

    Purpose

    3rd gen lentiviral vector for knocking down beta-catenin gene expression

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    b-catenin shRNA (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-C1-PKM2

    Plasmid

    #64698

    Purpose

    Expression of GFP tagged PKM2 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

    Insert

    PKM2 (PKM Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-B-GECO1

    Plasmid

    #32448

    Purpose

    Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    B-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

    Promoter

    CMV

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-wGBD

    Plasmid

    #26734

    Depositor

    William Bement

    Article

    Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

    Insert

    Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)
    

    Use

    Reporter for cdc42

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Cdc42 binding domain of N-WASP

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-4mtD3cpv

    Plasmid

    #36324

    Depositor

    Amy Palmer, Roger Tsien

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
    

    Use

    Tags

    First 29 aa of human COX subunit 8a

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFPV25.1

    Plasmid

    #20668

    Purpose

    S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

    Depositor

    Raphael Valdivia

    Article

    Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

    Type

    Empty backbone

    Use

    Tags

    GFP mut3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTetON-EGFP

    Plasmid

    #89453

    Purpose

    Tet/Dox-inducible expression of EGFP in mammalian cells (control)

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCS6-SMASh-YFP

    Plasmid

    #68852

    Purpose

    Expresses SMASh tag fused to YFP at N terminus in mammalian cells

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    SMASh-YFP
    

    Use

    Tags

    SMASh tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKH3-TRIM28

    Plasmid

    #45569

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

    Plasmid

    #52985

    Purpose

    split Venus attached to MCP and PCP respectively

    Depositor

    Robert Singer

    Article

    Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

    Insert

    nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    ubc

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEX-PK-hLC3

    Plasmid

    #61458

    Purpose

    Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy

    Depositor

    Isei Tanida

    Article

    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

    Insert

    PK-hLC3 (MAP1LC3B Fission Yeast, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. T4 Lysozyme WT

    Plasmid

    #18110

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FLAG-Stx17

    Plasmid

    #45911

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Dominant negative Hsp90 beta

    Plasmid

    #22480

    Depositor

    William Sessa

    Article

    Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

    Insert

    Dominant negative Hsp90 (HSP90AB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUAS-luc2

    Plasmid

    #24343

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Luciferase
    

    Use

    Tags

    5XGAL4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RFP-zyxin

    Plasmid

    #26720

    Depositor

    Anna Huttenlocher

    Article

    Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

    Insert

    Zyxin (ZYX Human, unknown)
    

    Use

    Tags

    RFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET His6 NusA TEV LIC cloning vector (1N)

    Plasmid

    #29657

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, NusA, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge