We narrowed to 10,941 results for: NSI;

1. pWM_12x601_193 NRL + TetO

   Plasmid

   #157812

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_tetO
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5_tAKARβ

   Plasmid

   #119914

   Purpose

   Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARβ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJYS1Peftu

   Plasmid

   #85546

   Purpose

   Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   etfu and unknown

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD2

   Plasmid

   #68161

   Purpose

   Universal domesticator plasmid for level 0 parts

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-NP-B/BRISBANE/60/2008

   Plasmid

   #187921

   Purpose

   Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   Influenza B Nucleoprotein
   

   Use

   Tags

   UNTAGGED

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE5400_pMBP_CDS dropout vector

   Plasmid

   #221189

   Purpose

   Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

   Depositor

   Tobias Erb

   Article

   Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

   Type

   Empty backbone

   Use

   Tags

   10x His-tag + E. coli maltose binding protein (MB…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1P shACO1

   Plasmid

   #169884

   Purpose

   Suppress ACO1

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   shRNA against ACO1
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP/KATNA1

   Plasmid

   #89316

   Purpose

   Expression of rat GFP/Katanin A subunit in mammalian cells

   Depositor

   Peter Baas

   Article

   Karabay et al J Neurosci. 2004 Jun 23;24(25):5778-88.

   Insert

   KATNA1 (Katna1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Shp2 biosensor

   Plasmid

   #134346

   Purpose

   FRET biosensor. To visualize Shp2 activity by FRET

   Depositor

   Yingxiao Wang

   Article

   Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

   Insert

   Shp2 Biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPBbsr2-Lifeact-miRFP703

    Plasmid

    #178530

    Purpose

    Expresses Lifeact-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Lifeact-miRFP703
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag Snail 6SA

    Plasmid

    #16221

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S96A, S100A, S107A, S111A, S115A, S119A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSMART-SOS-GFPuv

    Plasmid

    #102283

    Purpose

    Fluorescent sensor of E. coli SOS response

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    cda-GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cda SOS promoter

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJV53-Cas12a

    Plasmid

    #158706

    Purpose

    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-VEGFR2-780

    Plasmid

    #21307

    Depositor

    Donald Ingber

    Article

    Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

    Insert

    VEGFR2 promoter (KDR Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EFS-DIO-GRAB_ATP1.0

    Plasmid

    #167581

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2 stabilized mutant Beta Catenin-GFP

    Plasmid

    #29684

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S33A, S37A, T41A

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

19. Human RNF144B (27-236)

    Plasmid

    #110753

    Purpose

    Bacterial Expression of RNF144B RBR domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    RNF144B (RNF144B Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_Pro-IL-37

    Plasmid

    #214346

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL37 (IL37 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MAC_N_TBA1A

    Plasmid

    #111695

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    TBA1A (TUBA1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQC.Flip-nluc(LgBiT1-8)CP[TEV]

    Plasmid

    #200122

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCK01_PalkS_alkS

    Plasmid

    #166502

    Purpose

    Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

    Depositor

    Sven Panke

    Article

    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

    Insert

    alkS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    L401G

    Promoter

    PalkS

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMC-6-Cre-GR-9

    Plasmid

    #197723

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Cre recombinase with St-LS1 intron fused to glucocorticoid receptor
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMH610

    Plasmid

    #131862

    Purpose

    Produces holo-PhyB* in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

    Inserts

    PhyB(1-651)-RGD-His6-Cys
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGGS-SspB-mScarlet-I

    Plasmid

    #178521

    Purpose

    Expresses SspB-mScarlet-I in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    SspB-mScarlet-I
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET19b+H2B(T116C)

    Plasmid

    #157808

    Purpose

    Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H2B(T116C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T116C

    Promoter

    T7

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO human ULK1 shRNA 8

    Plasmid

    #27633

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    human ULK1 shRNA 8
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mNeonGreen-Smad3

    Plasmid

    #172098

    Purpose

    Expresses fluorescently tagged human Smad3 in mammalian cells.

    Depositor

    Lea Goentoro

    Article

    Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Smad 3 (Smad3 Mouse)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    C49S mutation in mCerulean

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHCA/GAL4(1-93).ER.VP16

    Plasmid

    #108216

    Purpose

    Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

    Depositor

    Didier Picard

    Article

    Louvion et al Gene 1993 Sep 6;131(1):129-34.

    Insert

    Gal4 DBD-ERalpha HBD-VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERalpha HBD has G400V mutation

    Promoter

    ADH1

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pegRNA_GBA-4

    Plasmid

    #199274

    Purpose

    pegRNA used to correct GBA (c. 1226 A > G) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    GBA 1226GtoA pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1(+)-ExRai-AKAR

    Plasmid

    #118405

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTnos (GB0037)

    Plasmid

    #68188

    Purpose

    Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Nos terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p416 25Q GPD

    Plasmid

    #1177

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. human MTAP

    Plasmid

    #64077

    Purpose

    Expression of human 5'-methylthioadenosine phosphorylase

    Depositor

    Vern Schramm

    Article

    Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

    Insert

    5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)
    

    Use

    Tags

    Histidine tag and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hsyn-GRAB_HA1mut

    Plasmid

    #190475

    Purpose

    Express the histamine-insensitive control sensor GRAB_HA1mut in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine-insensitive sensor GRAB_HA1mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid

    #115698

    Purpose

    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    SSKar2-sfpHluorin-HDEL
    

    Use

    Tags

    ER retention signal containing HDEL and ER signal…

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MCP-ADAR2-DD

    Plasmid

    #170124

    Purpose

    Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MAC_C_PDK1

    Plasmid

    #111672

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PDK1 (PDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSW610 - GR-LhG4_BD

    Plasmid

    #115992

    Purpose

    entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    GR-LhG4_BD
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge