We narrowed to 31,527 results for: Eng
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Plasmid#24982DepositorInsertHES1 (HES1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDNA-binding domain disrupted by E43A, K44A, R47A …PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJH4542
Plasmid#200779PurposePtwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptuneDepositorInsertGCaMP6
UseTagsmNeptuneExpressionWormMutationPromoterPtwk-40Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
dpYPQ230-D156R-ABE4
Plasmid#195370PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInsertecTadA8e-(GGGGS)x6-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCR5_gRNA_pX459
Plasmid#192268PurposegRNA expression for CRISPR Genome Editing of CCR5DepositorInsertCCR5 guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-sod1
Plasmid#107594PurposeContains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertsod1 (sod1 Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP95
Plasmid#83550PurposeUsed to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.DepositorInsertPDA1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPDA1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-pGAS5
Plasmid#46371DepositorInsertGAS5 (GAS5 Human)
UseLuciferaseTagsluciferaseExpressionMutationpromoterPromoterAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT for Split-PE (pEH45)
Plasmid#190105PurposeExpresses MMLV-RT (full-length, from PE2) for Split-PE useDepositorInsertMMLV-RT
UseTagsbpNLSExpressionMammalianMutationRT pentamutant from PE2 (D200N, T306K, W313F, T33…PromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-rnSOX9 ΔC (1-381)
Plasmid#62973PurposeExpresses HA-tagged rat SOX9 residues 1-383DepositorInsertSOX9 (Sox9 Rat)
UseTagsHAExpressionMammalianMutationcodes for residues 1-381PromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-Sesn2
Plasmid#61868PurposeTransient overexpression of Flag-Sesn2 (mouse)DepositorInsertSesn2 (Sesn2 Mouse)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB-HTB-ASK1
Plasmid#29517DepositorInsertASK1 (SKP1 Mustard Weed)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFB-GTE-COI1
Plasmid#29516DepositorInsertCOI1 (COI1 Mustard Weed)
UseTagsGST and TEV cleavage siteExpressionInsectMutationNote that the first AA in TEV site is R, not EPromoterAvailable sinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LAP1_2-mCherry
Plasmid#159526PurposeExpresses mCherry from LAP1_2 promoterDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterLAP1_2Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ef1a_LIN28_Ires_Puro
Plasmid#18921DepositorInsertLIN28 (LIN28A Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB WT
Plasmid#88886PurposeSCRIB WT in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralTagsN/AExpressionMutationN/APromoterMSCV promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1545_v1
Plasmid#126861Purpose4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1545_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
UseTagsExpressionWormMutationPromotereft-3Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLV-EF1a-V5-Smpd4
Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorInsertSmpd4 (Smpd4 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-LanCL2 (6xHis-LanCL2)
Plasmid#73407PurposeExpresses His tagged LanCL2DepositorInsertLanCL2 (LANCL2 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
3537 pMIG Bim
Plasmid#8786DepositorInsertBim (Bcl2l11 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
3540 pMIG Bid p15
Plasmid#8789DepositorInsertBid p15 (Bid Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH7
Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterADH2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCool-hDDB1-BPB
Plasmid#48639PurposeExpresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)DepositorInserthDDB1-BPB (DDB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
3538 pMIG Bad
Plasmid#8787DepositorInsertBad (Bad Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJH4687
Plasmid#200783PurposePnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorinDepositorInsertins-22 (ins-22 Nematode)
UseTagspHiuorinExpressionWormMutationPromoterPnpr-4Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-Akt1(WT)
Plasmid#86561PurposeExpresses human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds
Plasmid#119074PurposeZebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporterDepositorInsertubb:MCP-p65-HSF1-2a-Cerulean
UseTagsExpressionBacterialMutationPromoterZebrafish ubiquitin promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds
Plasmid#119075PurposeZebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporterDepositorInsertubb:Com-KRAB-2a-Cerulean
UseTagsExpressionBacterialMutationPromoterZebrafish ubiquitin promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-com-Ds
Plasmid#119077PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loopsDepositorInsertU6a:2xCom-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-MS2-Ds
Plasmid#119076PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loopsDepositorInsertU6a:2xMS2-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (Merlin)
Plasmid#136448PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EE-mut SV40 luc
Plasmid#8460DepositorInserthuman cyclin E promoter fragment, mutant (CCNE1 Human)
UseLuciferaseTagsExpressionMammalianMutationE2F sites I, II, and III mutated.PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionTagsExpressionMutationPromoterPol II readthrough transcription after integrationAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRKB134_GI-DUX4-intron2(-)
Plasmid#83968Purposeto test DUX4 mRNA is subjected to NMD upon intron2 inclusionDepositorInsertDUX4-3'UTR-intron2(-) (DUX4 Human)
UseTagsExpressionMammalianMutationPromotermouseCMVAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRKB133_GI-DUX4-intron2(+)
Plasmid#83967Purposeto test DUX4 mRNA is subjected to NMD upon intron2 inclusionDepositorInsertDUX4-3'UTR-intron2(+) (DUX4 Human)
UseTagsExpressionMammalianMutationPromotermouseCMVAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1EfmvaE>TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xHAtag-SP-PTPRO
Plasmid#191696PurposeFor expression coding sequencing of PTPRODepositorInsertsignal peptide, 3xHAtag and full length coding sequence of PTPRO
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdEos-VSVG-16
Plasmid#57693PurposeLocalization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVSVG
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-EF1a-V5-Sec61B
Plasmid#120244PurposeExpresses V5-tagged Sec61B in lentiviral vectorDepositorInsertSec61B (Sec61b Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Wnt3a (CT#169)
Plasmid#13976DepositorInsertWnt3a (Wnt3a Mouse)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Nxph4-HA
Plasmid#128102PurposeExpress HA tagged Nxph4DepositorInsertNxph4 (Nxph4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
2-Luc-Del-50
Plasmid#21730DepositorInsertp21 promoter deletion mutant (CDKN1A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationIdentical to 2-Luc except that the 50-bp region b…PromoterAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SP-CLSTN2-3xHAtag
Plasmid#191693PurposeFor expression coding sequencing of CLSTN2DepositorInsertsignal peptide, full length coding sequence of CLSTN2 and 3xHAtag
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3×FLAG-CMV-14-LanCL2
Plasmid#73391PurposeExpresses FLAG tagged LanCL2DepositorInsertLanCL2 (LANCL2 Human)
UseTags3x FLAG taggedExpressionMammalianMutationPromoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD
Plasmid#73300PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationN1043S-Q1047E-S1079N-E1083QPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCV-Rep-
Plasmid#32058DepositorInsertMCV-Rep- genome
UseTagsExpressionMutationSingle cytosine to adenine (C/A) mutation at 2,07…Promoternone from vector. Insert contains native promoter…Available sinceOct. 25, 2011AvailabilityAcademic Institutions and Nonprofits only