We narrowed to 32,226 results for: Eng
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Plasmid#42596DepositorInsertHepatitis B virus protein X
TagsFlagExpressionMammalianPromoterCMVAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLS-CMV-mP-Rluc
Plasmid#213507PurposeCMV-driven Renilla luciferase expression vector for luciferase lentivirus-based reporter assayDepositorInsertLuciferase
UseLentiviralAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-MT1-MMP-eGFP
Plasmid#89819PurposeExpress in MT1-MMP mammalian cellsDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH3830
Plasmid#200038PurposePrig-3 zif-1 SL2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of zif RFPDepositorAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L-R279E
Plasmid#182282PurposeMammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.DepositorInsertcAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorHas ServiceCloning Grade DNAInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L
Plasmid#182283PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L-R279E
Plasmid#182286PurposeMammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx120-20xMS2-Hyg
Plasmid#210220PurposeIntegrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
ires-eGFP(+HAs) donor-1
Plasmid#87865PurposeExpress eGFP reporter for HDR-induced knock-inDepositorInserteGFP
UseHomologous recombination for gapdh lociAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3V(1-10)
Plasmid#117483PurposeLentiviral vector for constitutive expression of sfCherry3V(1-10)DepositorInsertsfCherry3V(1-10)
UseLentiviralExpressionMammalianMutation6 substitutions (K45R, G52D, L83W, T106A, K182R, …PromoterSFFVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
3541 pMIG Bcl-XL
Plasmid#8790DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42229PurposeThis plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-EGFP-NLS
Plasmid#165441PurposeExpresses nuclear EGFP from CMV promoterDepositorInsertEGFP-NLS
UseAAVTagsNLSExpressionMammalianPromoterCMVAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-dCas9-KRAB
Plasmid#115545PurposeDoxycycline inducible dCas9-KRAB targeting construct for AAVS1 locusDepositorTypeEmpty backboneTagsHAExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFPI7
Plasmid#83555PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.DepositorInsertCUP1 promoter-yEGFP
ExpressionYeastPromoterCUP1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-M
Plasmid#182284PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-M (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-SpyTag-sfCherry2(11)-TagBFP
Plasmid#117485PurposeExpresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cellsDepositorInsertSpyTag002-GG-sfCherry2(11)
UseLentiviralTagsTagBFPExpressionMammalianPromoterSFFVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE4
Plasmid#195369PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInserthA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…ExpressionPlantPromoterZmUBI1Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-CDC25A
Plasmid#216511PurposeExpresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoterDepositorAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
Plasmid#117484PurposeExpresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cellsDepositorInsertSpyCatcher002-6aa-sfCherry2(1-10)
ExpressionMammalianPromoterCMVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher
Plasmid#117656PurposeBacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.DepositorInsertssfCherry2(1-10)
sfCherry2(11)-SpyCatcher002
ExpressionBacterialPromoterT7Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
CCR5_gRNA_pX459
Plasmid#192268PurposegRNA expression for CRISPR Genome Editing of CCR5DepositorInsertCCR5 guide RNA
UseCRISPRExpressionMammalianAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-bJunVN173
Plasmid#22012DepositorInsertbJunVN173 (Jun Rat, Aequorea Victoria)
TagsFLAGExpressionMammalianMutationc-Jun(257-338)-Venus(1-172)Available SinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hAsCpf1(TATV) (pY220)
Plasmid#89353PurposeExpresses humanized AsCpf1 variant that recognizes TATV PAMsDepositorInsertCpf1, RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCMVAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-H
Plasmid#182285PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-H (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1545_v1
Plasmid#126861Purpose4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1545_v1
UseSynthetic BiologyAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
3544 pMIG Bcl-2
Plasmid#8793DepositorAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1033_v1
Plasmid#126860Purpose4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1033_v1
UseSynthetic BiologyAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70 K71E
Plasmid#15216DepositorInserthsp70 K71E (HSPA1A Human)
TagsEGFPExpressionMammalianMutationK71E, ATPase-deficient mutant.Available SinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minPro
Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minProAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
ExpressionYeastPromoterGAL1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH4783
Plasmid#200785PurposePnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorinDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
tetO7_Ef1a_SOX2
Plasmid#18919DepositorAvailable SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minTK
Plasmid#211516PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minTKAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
ExpressionYeastPromoterTDH3Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
ExpressionYeastPromoterPGK1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p4B5-EGFP
Plasmid#140324PurposeDetection of Dengue virus infection in live cellsDepositorInsertDENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
TagseGFPExpressionMammalianMutation2 conservative n.t. differences near 5' end …PromoterCMVAvailable SinceMay 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
FlagETR3 (CELF2)
Plasmid#96900Purposeexpression of Flag-tagged human CELF2 (aka CUGBP2)DepositorAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Thy1 promoter construct
Plasmid#20736DepositorInsertThy1 promoter (Thy1 Mouse)
ExpressionMammalianAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT22
Plasmid#223394PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hAsCpf1(TYCV) (pY210)
Plasmid#89351PurposeExpresses humanized AsCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCMVAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionPromoterPol II readthrough transcription after integrationAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT19
Plasmid#223391PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.DepositorInsertAtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCG150_double_phiC31_attB
Plasmid#169693Purposecargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)DepositorTypeEmpty backboneExpressionWormAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10A
Plasmid#91207PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: noneDepositorInsert35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNICKclos1.0
Plasmid#73639PurposeGenome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824DepositorInsertsCas9 nickase
sGRNA to pyrE
UseE.coli-clostridium shuttle vectorMutationD10APromoterj23119 and ptbAvailable SinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only