165,744 results

1. Flag-HA-GFP

   Plasmid

   #22612

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18T-mini-Tn7T-Gm-Pc-sfGFP

   Plasmid

   #199248

   Purpose

   Fluorescent labelling of bacterial cells with sfGFP

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized sfGFP gene
   

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEcCas-2.0

   Plasmid

   #190697

   Purpose

   Constitutive expressing of Cas9 and inducible expression of lambda Red

   Depositor

   Sheng Yang

   Article

   Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

   Insert

   Cas9 and Lamda Red system
   

   Expression

   Bacterial

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6_empty-sgRNA(PP7)

   Plasmid

   #232432

   Purpose

   empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged sgRNA scaffold driven by human U6 promoter
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ8001 - pCMV-R8.91 (D64V)

   Plasmid

   #202687

   Purpose

   R8.91 plasmid with mutated integrase (D64V)

   Depositor

   Stanley Qi

   Article

   Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

   Insert

   gag pol
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   D64V

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD001 pME minprom_EGFP

   Plasmid

   #195955

   Purpose

   Gateway pME fluorophore

   Depositor

   Christian Mosimann

   Article

   Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

   Insert

   mouse beta-globin minimal promoter EGFP
   

   Use

   Other

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 APEX2-NES

   Plasmid

   #49386

   Purpose

   Expresses APEX2 in the cytosol of mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Flag - APEX2 - NES
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available Since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-fDIO mCherry

   Plasmid

   #114471

   Purpose

   Flp-dependent mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Promoter

   Ef1a

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTight-9-124-BclxL

   Plasmid

   #60857

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   pTight

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) NAPstar3b

    Plasmid

    #232736

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar3b
    

    Expression

    Mammalian

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-dTAG-Puro

    Plasmid

    #207727

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pORTMAGE-3

    Plasmid

    #72678

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-Cas9-T2A-GFP

    Plasmid

    #53190

    Purpose

    3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAS9-mCherry-Frame +0

    Plasmid

    #66939

    Purpose

    CRISPaint frame selector +0

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +0
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_PEmax-2A-BSD

    Plasmid

    #234071

    Purpose

    For lentiviral expression of PEmax with blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tn7 helper plasmid

    Plasmid

    #141161

    Purpose

    Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Insert

    Tn7 transposon machinery
    

    Expression

    Bacterial

    Promoter

    Arabinose-inducible pBAD

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330.puro

    Plasmid

    #110403

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPSU10-30-50-100

    Plasmid

    #173838

    Purpose

    Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-mCardinal

    Plasmid

    #51311

    Purpose

    Expresses far-red fluorescent protein mCardinal in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mCardinal
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ef1a_Large T-antigen_Ires_Puro

    Plasmid

    #18922

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    Large T Antigen (SV40gp6 Simian virus 40)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEM-3z/601

    Plasmid

    #26656

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Insert

    
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCnRed

    Plasmid

    #232908

    Purpose

    Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    gam (gam phage Lambda, Synthetic)
    

    Use

    Synthetic Biology

    Promoter

    Pbad-rha

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1-(empty)-TAG

    Plasmid

    #138209

    Purpose

    To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Type

    Empty backbone

    Use

    T7 transcription

    Tags

    TAG sequence

    Expression

    Mammalian

    Promoter

    CMV Promoter, T7 Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLG010 (DRT type 2)

    Plasmid

    #157888

    Purpose

    Expresses the DRT type 2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 2
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJRH-1187 VSVGmut

    Plasmid

    #201913

    Purpose

    Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    VSV-G (K47Q, R354A)
    

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCFD3-dU6:3gRNA

    Plasmid

    #49410

    Purpose

    expression of gRNA under control of the Drosophila U6:3 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-3:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMRXIP VPS16-GFP

    Plasmid

    #67023

    Purpose

    Human VPS16 ORF was inserted into pMRXIP GFP-N vector

    Depositor

    Noboru Mizushima

    Article

    Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

    Insert

    Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

28. GFP-HA-BACE1

    Plasmid

    #165032

    Purpose

    HA-BACE1

    Depositor

    Juan Bonifacino

    Article

    Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

    Insert

    BACE1 (BACE1 Human)
    

    Tags

    HA (embedded after propeptide cleavage site)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. pXPR_219

    Plasmid

    #164559

    Purpose

    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

    Inserts

    First sgRNA cassette
    Second sgRNA cassette
    

    Use

    CRISPR and Lentiviral; Dual sgrna

    Expression

    Mammalian

    Promoter

    Human U6 and Mouse U6

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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