4,133 results
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pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVUHshp53
Plasmid#11653DepositorInsertGFP, shRNA against TP53 (TP53 Human)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-mKate2 caspase-3 biosensor
Plasmid#37132DepositorInsertLSSmOrange-DEVD-mKate2
UseTagsExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin A
Plasmid#8959DepositorInsertcyclin A (CCNA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE-bleo IGF-1R
Plasmid#11212DepositorInsertIGF-1R (IGF1R Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
UseTagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-mito-mCherry-10xGCN4_v4
Plasmid#60914PurposeA fusion protein of the SunTag10xGCN4 v4 peptide array to a mitochondrial targeting domain and mCherryDepositorInsertmito-mCherry
UseTagsExpressionMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE BRG1
Plasmid#1959DepositorInsertBRG1 (SMARCA4 Human)
UseRetroviralTagsflagExpressionMammalianMutationE1262K R1575QPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1-HA
Plasmid#24399DepositorInsertBeclin1 (BECN1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry
Plasmid#78534PurposeBackbone to clone single or paired sgRNAsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6 (for expressing sgRNA)Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Gamma-Tubulin-17
Plasmid#55050PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
UbcH5c WT pET28a
Plasmid#12643DepositorInsertUbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSK+KanaRpsL
Plasmid#20871DepositorInsertKanamycinResistance-RpsL
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
1013 pcDNA3 T7 Akt1 T308A S473A
Plasmid#9030DepositorInsertAkt1 T308A S473A (AKT1 Human)
UseTagsExpressionMammalianMutationT308A S473APromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pCMV-7.1
Plasmid#47948PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840A (catalyticall…PromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-Lifeact-7
Plasmid#54694PurposeLocalization: Actin, Excitation: 490 / 553, Emission: 507 / 573DepositorTypeEmpty backboneUseTagsDendra2-LifeactExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
p-cyclinB1-mcherry (1958)
Plasmid#26063DepositorInsertCyclin B1 (CCNB1 Human)
UseTagsmcherryExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-RanQ69L
Plasmid#30309DepositorInsertRanQ69L (RAN N/A)
UseTagsFLAG-CherryExpressionMammalianMutationGlutamine 69 is changed to LeucinePromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic EKAR (EGFP-mRFP)
Plasmid#18680DepositorInsertERK Activity Reporter
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pZW6 (CK2alpha)
Plasmid#27086DepositorInsertCK2alpha (CSNK2A1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
Plasmid#64512PurposeExpression of St1 dCas9-3XTagBFP2 in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XmTagBFP2ExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_LIN28A
Plasmid#26819DepositorInsert5'UTR-LIN28A-3'UTR (LIN28A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKMyc
Plasmid#19400DepositorTypeEmpty backboneUseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hMYC
Plasmid#20723DepositorInsertc-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
AmCyan-P2A-mCherry
Plasmid#45350DepositorInsertAmCyan
UseTagsmCherryExpressionMammalianMutationSV40 NLSPromoterCMVAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-GFP-NMHC II-B
Plasmid#11348DepositorInsertNMHC II-B (MYH10 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDsRed Monomer C1-YAP2
Plasmid#19057DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsRed-FPPExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only
