We narrowed to 9,308 results for: control

1. pRDA_834

   Plasmid

   #216087

   Purpose

   Cas9 [Sp] knockout targeting CD59, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD59 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIBN-tagBFP-hPMLIII

   Plasmid

   #103807

   Purpose

   BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-tagBFP-hPMLIII (CIB1, PML Human, Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDC32B-AtTAS1c-D2-B/c

   Plasmid

   #137884

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]

   Depositor

   Alberto Carbonell

   Article

   Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

   Insert

   AtTAS1c-D2-B/c
   

   Expression

   Plant

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   2x35S

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #47455

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Use

   AAV

   Tags

   2A_GFP

   Expression

   Mammalian

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pgRGFP

   Plasmid

   #82695

   Purpose

   Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

   Depositor

   Alan Mullen

   Article

   Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK, U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6- Entry-RPL26DC-DDK

   Plasmid

   #133867

   Purpose

   Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   RPL26DC-myc-FLAG (RPL26 Human)
   

   Tags

   Myc-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG

   Plasmid

   #176396

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   GAL1 promoter, SZ1-RGG-GFP-RGG-RGG
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN-X-hPGK-PuroR-Z

   Plasmid

   #218904

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   hPGK-PuroR
   

   Expression

   Mammalian

   Mutation

   246T>A and 398T>C

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PU.1-ets-IRES-mCherry

    Plasmid

    #80141

    Purpose

    retroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherry

    Depositor

    Ellen Rothenberg

    Article

    Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

    Insert

    PU.1 (Spi1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    ets = PU.1 DNA binding domain (amino acids 159-26…

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-Hygro-rKIF5B-GFP

    Plasmid

    #210335

    Purpose

    Overexpression of rKIF5B

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    rKIF5B (Kif5b Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1Xpress-AbrGAP

    Plasmid

    #38177

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    Abr GAP domain (ABR Human)
    

    Tags

    Xpress

    Expression

    Mammalian

    Mutation

    ABR GAP domain including amino acids 597-859

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pFastBac-His6-Mps1-WT

    Plasmid

    #114063

    Purpose

    protein production

    Depositor

    Geert Kops

    Article

    Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

    Insert

    Mps1-WT (TTK Human)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    none

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-RIPK3 K50A

    Plasmid

    #78817

    Purpose

    To overexpress RIPK3 K50A in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K50A

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCALNL-Kir2.1

    Plasmid

    #125578

    Purpose

    Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAN200

    Plasmid

    #129203

    Purpose

    yTRAP sensor of [PSI+], detects aggregation of Sup35

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35NM (SUP35 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    N terminal and middle domains

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRV FOXP3 WRR

    Plasmid

    #13624

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Insert

    FOXP3 (FOXP3 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    WRR: N361W, E399R, E401R

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

19. mito-yHS1-M7A

    Plasmid

    #159164

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A
    

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of mito-yHS1…

    Promoter

    GPD

    Available Since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAAV-shNCLX-2

    Plasmid

    #181871

    Purpose

    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    solute carrier 8 family member B1 (Slc8b1 Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-shNCLX-1

    Plasmid

    #181870

    Purpose

    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    solute carrier 8 family member B1 (Slc8b1 Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pInsert-PhoCl-RER

    Plasmid

    #219676

    Purpose

    Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    CD4mScarlet-PhoCl-RER
    

    Tags

    mScarlet and PhoCl

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1417 (No intron)

    Plasmid

    #69898

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Expression

    Mammalian

    Mutation

    F169L in ZKSCAN1

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ECFP-Bcr in pCCL-cppt178-MNDU3

    Plasmid

    #38156

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    Bcr (BCR Human)
    

    Use

    Lentiviral

    Tags

    ECFP

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pPHR-mCherry-hPMLIII

    Plasmid

    #103824

    Purpose

    Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-hPMLIII (CRY2, PML Human, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-HDC-DIO-GFP-shRNA.scramble

    Plasmid

    #184331

    Purpose

    Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    hdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and RNAi

    Expression

    Mammalian

    Promoter

    histidine decarboxylase promoter fragment that g…

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mApple-MyosinIIA-N-18

    Plasmid

    #54930

    Purpose

    Localization: Cytoskeleton, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

    Insert

    MyosinIIA
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

    Plasmid

    #176400

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    FRB-RGG-GFP-RGG-RGG
    

    Tags

    FRB and GFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJET1.2-U6

    Plasmid

    #173157

    Purpose

    Single guide RNA cassette under U6 promoter

    Depositor

    Piotr Ziolkowski

    Article

    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

    Insert

    sgRNA cassette under U6 promoter
    

    Expression

    Plant

    Promoter

    AtU6-26

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLXSN-H-Ras_V12

    Plasmid

    #39516

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pGEX4T1-GST-LC3B

    Plasmid

    #216836

    Purpose

    For expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    LC3B (Gly exposed) (MAP1LC3B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    TAC promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCI-FLAG-mRRP6

    Plasmid

    #60045

    Purpose

    Mammalian expression of FLAG-tagged mouse RRP6

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    RRP6 (Exosc10 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pB80-KIF1A(1-383)-GFP-SSPB(milli)

    Plasmid

    #174626

    Purpose

    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)
    

    Tags

    GFP-SSPB(milli)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCIBN-tagBFP-TetR

    Plasmid

    #103797

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-TetR (CIB1, tetR E. coli, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Expression

    Bacterial and Yeast

    Promoter

    TDH3

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MSCV Puro SCRIB P305L

    Plasmid

    #88887

    Purpose

    SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Mutation

    Proline to Leucine mutation @ 305th amino acid

    Promoter

    MSCV promoter

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-CreLite

    Plasmid

    #131785

    Purpose

    AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC-P2A-PIF6CreN
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

    Plasmid

    #107727

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEM-HE-Venus-bPAC(F198Y)

    Plasmid

    #165487

    Purpose

    Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Venus-bPAC(F198Y)-myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDisplay-GRAB_OTmut

    Plasmid

    #185385

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge