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167,150 results

Showing: 4681 - 4720 of 167150 results

  5. pcDNA3.1-Pur-mEGFP

    Plasmid
    #186329
    Purpose
    Fluorescent reporter system
    Depositor
    Bertrand Collet
    Insert
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186329-plasmid-map-sequence-id-400611

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  7. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  16. hNTo1-qgRNA-pYJA5

    Plasmid
    #217781
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217781-plasmid-map-sequence-id-466019

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  17. EF1α-eGFP-Z1

    Plasmid
    #135612
    Purpose
    Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    eGFP
    Expression
    Mammalian
    Promoter
    EF1a-HTLV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135612-plasmid-map-sequence-id-269403

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  19. pSBtet-RP

    Plasmid
    #60497
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60497-plasmid-map-sequence-id-97550

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  26. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has Service
    AAV Retrograde and AAV8
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154868-plasmid-map-sequence-id-308827

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  27. AbVec2.0-mIghg1

    Plasmid
    #127158
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg1 (Ighg1 Mouse)
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127158-plasmid-map-sequence-id-256842

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  35. pFlag-CMV1-hMD2

    Plasmid
    #13028
    Depositor
    Doug Golenbock
    Insert
    MD2 (LY96 Human)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13028-plasmid-map-sequence-id-169271

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  37. p667-UBC-CDK12-V5-miniTurbo_IDG-K

    Plasmid
    #135243
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-miniTurbo (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135243-plasmid-map-sequence-id-269114

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  38. AD59_pEP.DonorCLYBL.TS

    Plasmid
    #199227
    Purpose
    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    Use
    Gene targeting donor plasmid
    Expression
    Mammalian
    Promoter
    Human PGK1 gene promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199227-plasmid-map-sequence-id-391392

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Showing: 4681 - 4720 of 167150 results