167,150 results
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pJAT30
Plasmid#204294PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.DepositorInsertMHC-DsRed
UseCRISPRPromoterMHCAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R
Plasmid#58978Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
ExpressionBacterialPromoterT7/Sp6Available SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF1374
Plasmid#143955PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-lexAp65
Plasmid#138393PurposeRMCE vector for generating C-terminal T2A-lexA-p65 fusionDepositorInsertT2A-lexAp65
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
ExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…Available SinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330.puro
Plasmid#110403PurposeCRISPR/Cas9 vector with sgRNA expression and puromycin resistanceDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCBhAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChrimsonR-tdT
Plasmid#59171PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianPromoterSynAvailable SinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP-RPS5-C1
Plasmid#134534PurposeExpresses YFP-RPS5 fusion proteinDepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-CLIP170-C-18
Plasmid#54043PurposeLocalization: Microtubule Binding , Excitation: 487, Emission: 509DepositorAvailable SinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0656
Plasmid#141653PurposeLentiviral vector for overexpressing the CHD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-dCas9-5xPlat2AflD
Plasmid#82560PurposeExpression vector for Cas9 peptide array (linker length: 22aa).DepositorInsertdCas9-5xPlat2AflD
ExpressionMammalianAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-X
Plasmid#81071PurposeEmpty lentiviral backbone with the MNDU3 promoterDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterMNDU3Available SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-Rab7
Plasmid#174458PurposeExpress mCherry with late endosome-targeting sequence in mammalian cellsDepositorInsertmCherry with late endosome-targeting sequence
Tagssmall GTPase (Rab7)ExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSTING (N154S)
Plasmid#214160PurposeTransient expression of gain-of-function STING mutantDepositorAvailable SinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-HA-FLAG-AU1
Plasmid#162118PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to HA-FLAG-AU1
UseLentiviralTagsHA-FLAG-AU1MutationWTPromoterEF1AAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM496 (deletions) Zeo
Plasmid#109301PurposeORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.DepositorInsertBxb1 attB site
UseOrbit integrating plasmidAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
CoV2-M-IRES-E
Plasmid#177938PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorExpressionMammalianAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM1414
Plasmid#40237DepositorInsertpromoterless luxAB reporter
UseCyanobacteria cloning vectorPromotern/aAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHel2
Plasmid#102960PurposeE.coli/H.Pylori shuttle vectorDepositorTypeEmpty backboneUsePlasmid shuttle vector system for genetic complem…Available SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-jRCaMP1a
Plasmid#113936PurposeBacterial expression of red fluorescent Ca2+ sensor jRCaMP1aDepositorInsertjRCaMP1a
Tags6xHisExpressionBacterialAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK1A(K188R)-2A-mCherry
Plasmid#177166PurposeExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationChanged Lysine 188 to ArgininePromoterCMV with beta globin intronAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-mCherry
Plasmid#217005Purposelentiviral plasmid for gRNA cloning with selectable mCherry expressionDepositorTypeEmpty backboneUseLentiviralAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TERT - WT
Plasmid#213827PurposeExpresses human telomerase reverse transcriptase, the catalytic subunit of telomeraseDepositorInserthuman TERT (TERT Human)
UseLentiviralExpressionMammalianPromoterTet-responsive promoter PTight, consisting of sev…Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM468-EGFP
Plasmid#108434PurposeORBIT integrating plasmid for C-terminal tagging with EGFP.DepositorInsertattB-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-BRD7
Plasmid#65379PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP
Plasmid#176283PurposeRecombinant rabies virus expressing histone-EGFPDepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC21
Plasmid#49787PurposeE. coli cloning vector (AmpR, high copy, blue/white selection, M13 IR)DepositorTypeEmpty backboneUseCloning vectorAvailable SinceJuly 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET mCherry LIC cloning vector (u-mCherry)
Plasmid#29769DepositorTypeEmpty backboneTagsmCherryExpressionBacterialPromoterT7Available SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaQ-RLuc8
Plasmid#140982PurposeEncodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaQ-RLuc8 (GNAQ Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001148320)
Plasmid#76378Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)
Plasmid#48296DepositorTypeEmpty backboneTagsHis6 and MBPExpressionInsectAvailable SinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJH773_pTech_PlldR-HHRz-supP(opal)
Plasmid#163732PurposeLactate-inducible expression of supP(opal), preceded by a hammerhead ribozymeDepositorInserthhrz-supP
ExpressionBacterialAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSynapsin-Cre-WPRE
Plasmid#86641PurposeExpresses Cre in mammalian cellsDepositorInsertCre
UseCre/Lox, Lentiviral, Mouse Targeting, and Synthet…ExpressionMammalianPromoterhSynapsinAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC1-REX-YFP
Plasmid#48247PurposeGenetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertREX-YFP
ExpressionMammalianPromoterCMVAvailable SinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSP90AA1
Plasmid#177658PurposeRecombinant protein synthesis of human HSP90AA1DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10b-EGFP-KRAS-WT
Plasmid#164935PurposeExpresses EGFP cDNA and KRAS wild type cDNA in mammalian cellsDepositorInsertsUseLentiviralPromoterUbc Promoter and Ubc promoterAvailable SinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab11
Plasmid#174460PurposeExpress iRFP with recycling endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with recycling endosome-targeting sequence
Tagssmall GTPase (Rab11)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh
Plasmid#207634PurposeA plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.DepositorInsertphotocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
ExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LTBP1-bio-His
Plasmid#53421PurposeExpresses full-length Latent-transforming growth factor beta-binding protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertLTBP1 (LTBP1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-P4Mx1
Plasmid#108143PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
ExpressionMammalianPromoterCMVAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2515
Plasmid#91083PurposeModule C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRPromoterAtU6Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CCL21-V5
Plasmid#11609DepositorAvailable SinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK12_IDG-K
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
M23: pHR-SFFVp-FUSN::mCherry::SspB
Plasmid#122148PurposeN terminal domain of FUS (1-214) tagged with SspB and mCherry.DepositorInsertFUS (FUS Human)
UseLentiviralTagsmCherry-sspBExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
TagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23a-HsRPS27a
Plasmid#69561PurposeBacterial expression; in vitro transcription/translation (T7)DepositorAvailable SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC7A13_STOP
Plasmid#161327PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC7A13 (SLC7A13 Human)
ExpressionMammalianAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CAG-RiboL1-jGCaMP8f
Plasmid#192612PurposeAAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.DepositorInsertRiboL1-jGCaMP8f
UseAAVTags6xHisExpressionMammalianAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only
