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20,197 results

Showing: 5001 - 5040 of 20197 results

  1. pHIV-dTomato

    Plasmid
    #21374
    Purpose
    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    21374-plasmid-map-sequence-id-292244

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  3. GFP-C1-PLCdelta-PH

    Plasmid
    #21179
    Purpose
    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids
    Depositor
    Tobias Meyer
    Article
    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.
    Insert
    PLCdelta (PLCD1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    PH domain of human PLC delta, amino acids Met1–Il…
    Available Since
    July 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21179-plasmid-map-sequence-id-212104

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  9. CHRM3-Tango

    Plasmid
    #66250
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CHRM3 (CHRM3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pRRLsinPGK_IRFP720_WPRE

    Plasmid
    #91785
    Purpose
    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    iRFP720
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91785-plasmid-map-sequence-id-180617

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  16. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

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  25. eIF2a 1

    Plasmid
    #21807
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Available Since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21807-plasmid-map-sequence-id-351672

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  26. pV1093

    Plasmid
    #111428
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111428-plasmid-map-sequence-id-212463

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  32. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Yeast
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100539-plasmid-map-sequence-id-194476

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Showing: 5001 - 5040 of 20197 results