19,200 results

1. AAV-CMV-Flag-PGC1a-6His

   Plasmid

   #67637

   Purpose

   AAV vector expressing PGC1a gene

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   PGC1a (Ppargc1a Mouse)
   

   Use

   AAV

   Tags

   6XHis and Flag tag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Vinculin-venus

   Plasmid

   #27300

   Depositor

   Martin Schwartz

   Article

   Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

   Insert

   Vinculin (VCL Chicken)
   

   Use

   Tags

   Venus A206K

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST-V1-ORF

   Plasmid

   #73635

   Purpose

   Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Use

   Tags

   Venus fragment 1 (V1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTrcHIS-wt7D12

   Plasmid

   #125268

   Purpose

   Expresses wild type 7D12 anti-EGFR nanobodies

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   7D12 nanobody
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Trc

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMD.RVG.CVS24-N2c

   Plasmid

   #19712

   Depositor

   Manfred Schubert

   Article

   Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

   Insert

   Rabies virus CVS24-N2c glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLJM1-Empty

   Plasmid

   #91980

   Purpose

   Empty backbone

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST-V2-ORF

   Plasmid

   #73636

   Purpose

   Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Use

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.3_d2eGFP

   Plasmid

   #26821

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Sep 29. ():.

   Insert

   5'UTR-d2eGFP-3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   destabilized (PEST sequence) GFP

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pBb(RSF1010)1k-GFPuv

   Plasmid

   #106395

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-TA-ERN

    Plasmid

    #80474

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRc/CMV2 Dvl-2-Myc

    Plasmid

    #42194

    Depositor

    Robert Lefkowitz, Shin-ichi Yanagawa

    Article

    Lee et al J Biol Chem. 1999 Jul 23;274(30):21464-7

    Insert

    Dvl 2 (Dvl2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMMTV neu NT

    Plasmid

    #1823

    Depositor

    Philip Leder

    Article

    Muller et al Cell 1988 Jul 1;54(1):105-15.

    Insert

    neu NT (Erbb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-FLEX-GFP

    Plasmid

    #28304

    Depositor

    Edward Boyden

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCB591

    Plasmid

    #141096

    Purpose

    E. coli-Lactobacilli shuttle vector for recombineering template cloning

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-FLEX-EGFP-KASH

    Plasmid

    #154373

    Purpose

    Vector for Cre-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-roxSTOProx-ZsGreen

    Plasmid

    #51274

    Purpose

    Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    roxSTOProx-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHIV-dTomato

    Plasmid

    #21374

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. HP4_M13

    Plasmid

    #120340

    Purpose

    Helper plasmid for production of phagemid ssDNA, based on m13mp18

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Coding part of the M13 phage genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native phage promoters

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 2Flag-pDEST-N

    Plasmid

    #118371

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CMV500 empty vector

    Plasmid

    #33348

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PB533-42B3mutAC2-sfGFP

    Plasmid

    #186777

    Purpose

    RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

    Insert

    42B3mutAC2-scFv
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE-TO-dCas9-3XmCherry

    Plasmid

    #64108

    Purpose

    Expression of Sp dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MTS-mCherry-GFP1-10-Hyg-N1

    Plasmid

    #91957

    Purpose

    Expresses mCherry-GFP1-10 in mammalian cell mitochondria

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    MTS-mCherry-GFP1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Ubc.Luc.IRES.Puro

    Plasmid

    #33307

    Depositor

    Linzhao Cheng

    Article

    Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21a-GlnRS-His

    Plasmid

    #124108

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutaminyl-tRNA ligase (glnS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA1-Tat

    Plasmid

    #138478

    Purpose

    Expresses HIV Tat for efficient sgRNA packaging.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Tat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge