We narrowed to 50,495 results for: ang

1. pYPQ230-D156R

   Plasmid

   #188542

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-D156R
   

   Use

   CRISPR; Gateway compatible lbcas12a-d156r entry c…

   Tags

   NLS

   Expression

   Plant

   Mutation

   D156R

   Promoter

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

   Plasmid

   #192133

   Purpose

   Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9-KY
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Hsp1-Hsp2Cas9 (K390A, Y446A)

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

   Plasmid

   #192132

   Purpose

   Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9-Y
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Hsp1-Hsp2Cas9 (Y446A)

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV6-ZNF354C_mutant_M41K-L42K-E43K

   Plasmid

   #194185

   Purpose

   Expresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)

   Depositor

   Matthias Leisegang, Ralf Brandes

   Article

   Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

   Insert

   ZNF354C (ZNF354C Human)
   

   Use

   Tags

   Myc-DDK

   Expression

   Mammalian

   Mutation

   KRAB domain mutant M41K/L42K/E43K

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-3x-NLS-mScarlet-AMPKα2

   Plasmid

   #192452

   Purpose

   mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
   

   Use

   Tags

   3x Nuclear localization signal (NLS) and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBH-Cas9-Suntag-BB_BbsI

   Plasmid

   #164805

   Purpose

   REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMKO.1-shNuf2

   Plasmid

   #160961

   Purpose

   Nuf2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nuf2 shRNA (Nuf2 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUASp-Flag-Myc-Qin-Tudor-attB

   Plasmid

   #44210

   Depositor

   Phillip Zamore

   Article

   Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

   Insert

   Qin-Tudor-domain (qin Fly)
   

   Use

   Fly transformation

   Tags

   Flag and Myc

   Expression

   Mutation

   lacks E3 domain on N-term (del aa1-274)

   Promoter

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSP64-hERG1a D540K

   Plasmid

   #53057

   Purpose

   Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Aspartate 540 to Lysine

   Promoter

   SP6

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cs2+Etv4 S100D

    Plasmid

    #49094

    Purpose

    expression of Etv4 S100D mutant

    Depositor

    Michael Tsang

    Article

    Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

    Insert

    Etv4 S100D (etv4 Zebrafish)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    Expression

    Mammalian

    Mutation

    S100D

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV10/3xflag-Clock L113E

    Plasmid

    #47339

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L113E

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCMV10/3xflag-Clock F122D

    Plasmid

    #47340

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    F122D

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3-HA/BmalF423R

    Plasmid

    #47348

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    F423R

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

14. piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

    Plasmid

    #209081

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T, NAC deletion (aa60-95)

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-CRISPRv2-sgNTC13-hygro

    Plasmid

    #231988

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Integrin β3-GFP

    Plasmid

    #205091

    Purpose

    Express GFP-tagged human integrin β3 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 3 (ITGB3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pINDUCER dCas9-TET1CD

    Plasmid

    #101921

    Purpose

    The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DYRK1A/pTRE3G-FL-hXIST

    Plasmid

    #149608

    Purpose

    Express doxycycline-inducible full length of human XIST cDNA

    Depositor

    Jeanne Lawrence

    Article

    Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

    Insert

    X-inactive specific transcript (XIST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE3G

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSMT3_SRSF1

    Plasmid

    #201056

    Purpose

    Express sumo-tagged human SRSF1

    Depositor

    Jun Zhang

    Article

    Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

    Insert

    SRSF1 (SRSF1 Human)
    

    Use

    Tags

    sumo

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

    Plasmid

    #75470

    Purpose

    Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Reversed ChR2(H134R)-mCherry
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDY1173_ADAR1p150

    Plasmid

    #193192

    Purpose

    Express full length ADAR1p150

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    ADAR1p150 (ADAR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLQ1658-dCas9-EGFP

    Plasmid

    #51023

    Purpose

    Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    dCas9 fuse to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

    Plasmid

    #199605

    Purpose

    Expression of cell permeable opAsCas12a in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    opCas12a-T8N
    

    Use

    CRISPR

    Tags

    6xHis, Twin-strep, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-CRISPRv2-sgNTC49-puro

    Plasmid

    #231989

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral vector

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

    Plasmid

    #231986

    Purpose

    Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    OVA-2A-ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

    Plasmid

    #121966

    Purpose

    Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    superfold GFP-GB1-NLS-iLID

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19-dual-scafffold-2.0 (pBP49)

    Plasmid

    #218931

    Purpose

    The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Insert

    dual scaffolds for CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Dual-CRISPR-Cas9-U6-H1 (pBP48)

    Plasmid

    #218932

    Purpose

    The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    2A-Puro and 3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

    Plasmid

    #199604

    Purpose

    Expression of cell permeable spCas9 in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    Cas9-T6N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Hygro-Cas9 donor

    Plasmid

    #86883

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

    Depositor

    Danwei Huangfu

    Article

    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-PE5max-SF3B1-K700E

    Plasmid

    #200317

    Purpose

    Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Inserts

    K700E-ng1+pg2
    PEmax-2A-MLH1dn
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOD2045-intControl

    Plasmid

    #100005

    Purpose

    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Inserts

    zif-1 (zif-1 Nematode)
    unc-119
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-CAG-(n1)QC2-6 FIQ(c4)-P2A-FusionRed

    Plasmid

    #229573

    Purpose

    Expresses (n1)QC2-6 FIQ(c4) and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    (n1)QC2-6 FIQ(c4)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGP-AAV-syn-flex-iGABASnFR2-WPRE

    Plasmid

    #218877

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    Synapsin

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-DIO-EF1α-mKate2-biPAC

    Plasmid

    #169127

    Purpose

    AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-biPAC
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    codon optimized to mouse

    Promoter

    EF1a

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1/CIP2A(1-905) WT V5 His

    Plasmid

    #119287

    Purpose

    CIP2A WT V5 overexpression

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCDH-CMV-FLAG-CFP-AMOT

    Plasmid

    #235682

    Purpose

    Express CFP tagged AMOT gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    AMOT (AMOT Human)
    

    Use

    Lentiviral

    Tags

    FLAG-CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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42. FAT FAK biosensor

    Plasmid

    #78303

    Purpose

    FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

    Insert

    FAT FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

43. Integrin β4-GFP

    Plasmid

    #205092

    Purpose

    Express GFP-tagged human integrin β4 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 4 (ITGB4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCDH-CMV-V5-RFP-SLMAP (isoform r)

    Plasmid

    #235685

    Purpose

    Express RFP tagged SLMAP gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    SLMAP (SLMAP Human)
    

    Use

    Lentiviral

    Tags

    V5-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239024

    Purpose

    Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    Bo Huang, William DeGrado

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    Rhobin2-SEC61B (SEC61B Human, Synthetic)
    

    Use

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDest17_MAX

    Plasmid

    #234044

    Purpose

    Bacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage site

    Depositor

    Tanja Mittag

    Article

    Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

    Insert

    MAX (MAX Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pRK5-FLAG-SLC38A9.1

    Plasmid

    #71855

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDY1462_SERPINA1_CCA32_Caspase

    Plasmid

    #193194

    Purpose

    Express Caspase conditional upon RNA transcript

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    iCaspase9 (CASP9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. TBG-Vector

    Plasmid

    #109396

    Purpose

    AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver

    Depositor

    Sidi Chen

    Article

    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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