161,641 results

1. pCMV-HyPer7-MEM

   Plasmid

   #136465

   Purpose

   Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Farnselation tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Plasmid

   #179105

   Purpose

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p2UGFP

   Plasmid

   #1195

   Depositor

   Susan Lindquist

   Article

   Patino et al Science 1996 Aug 2;273(5275):622-6.

   Insert

   GFP w/ stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pAAV.GFAP.eGFP.WPRE.hGH

   Plasmid

   #105549

   Purpose

   AAV expression of EGFP from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV5

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL-p27UTR

   Plasmid

   #20881

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p27 3'UTR (Cdkn1b Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-Target-ACEmax

   Plasmid

   #139105

   Purpose

   Mammalian Target-ACEmax expression vector (pSI-625)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CBX3 A6.5 gRNA

   Plasmid

   #90569

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CBX3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CBX3 (Guide Designation A6.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-mAmetrine-CNA35

   Plasmid

   #61604

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mAmetrine and CNA35
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. IVT-donor_opt(R2Tg)-GFP

   Plasmid

   #223246

   Purpose

   In vitro transcription of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. IVT-R2Tg_opt

    Plasmid

    #223247

    Purpose

    In vitro transcription of the optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CBX3 A5.5 gRNA

    Plasmid

    #90568

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CBX3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CBX3 (Guide Designation A5.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330-EN1201

    Plasmid

    #92144

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTET GFP10-FRB/FKBP-GFP11

    Plasmid

    #182497

    Purpose

    Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Inserts

    FRB
    FKBP (Fkbp1a Synthetic, Mouse)
    

    Use

    Tags

    split GFP10 tripartite and split GFP11 tripartite

    Expression

    Bacterial

    Mutation

    Promoter

    TET

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti_P2A_Puro

    Plasmid

    #211364

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Article

    Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Sema4D-Flag

    Plasmid

    #190646

    Purpose

    Expresses Mouse Sema4D with C Terminal Flag Tag

    Depositor

    Andrew Newman, Victor Tarabykin

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-sp-myc-mSema4D

    Plasmid

    #190645

    Purpose

    Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

    Depositor

    Andrew Newman, Victor Tarabykin

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1_SybBC10

    Plasmid

    #240997

    Purpose

    Expression of sybody SLC66A1 in mammalian cells

    Depositor

    Simon Newstead

    Insert

    Syb-BC10-Myc-His6
    

    Use

    Tags

    Myc and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NanoBRET Assay Vector, MYC

    Plasmid

    #237142

    Purpose

    Express Myc-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    Myc (MYC Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. LgBiT-PIK3CA Fusion Vector

    Plasmid

    #237092

    Purpose

    Express LgBiT-PIK3CA Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. SmBiT-KRAS 4B G12C BiBiT Ready Vector

    Plasmid

    #237079

    Purpose

    Express SmBiT-KRAS 4B G12C Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. Nluc-YAP1_TEAD4-HaloTag Bidirectional(Balanced) Vector

    Plasmid

    #237003

    Purpose

    Express NLuc-YAP1_TEAD4-HaloTag(R) Fusion Proteins in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    YAP1 (YAP1 Human)
    TEAD4 (TEAD4 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pIL6-Nluc-hbb

    Plasmid

    #239843

    Purpose

    Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.

    Depositor

    Catherine Jopling

    Article

    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

    Insert

    Secreted nanoluciferase, with interleukin 6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 (included as part of insert)

    Available since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQAT22

    Plasmid

    #223394

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJethlpAmScarletCm

    Plasmid

    #206818

    Purpose

    Template for hlpA-mScarlet-I amplification associated with chloramphenicol resistance gene

    Depositor

    Virginie Libante

    Article

    Mignolet et al bioRxiv 2025.05.10.653270

    Insert

    hlpA
    

    Use

    Synthetic Biology

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJethlpAYFPCm

    Plasmid

    #206826

    Purpose

    Template for hlpA-YFP amplification associated with chloramphenicol resistance gene

    Depositor

    Virginie Libante

    Article

    Mignolet et al bioRxiv 2025.05.10.653270

    Insert

    hlpA
    

    Use

    Synthetic Biology

    Tags

    YFP

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJethlpAmNeonGreenCm

    Plasmid

    #206814

    Purpose

    Template for hlpA-mneongreen amplification associated with chloramphenicol resistance gene

    Depositor

    Virginie Libante

    Article

    Mignolet et al bioRxiv 2025.05.10.653270

    Insert

    hlpA
    

    Use

    Synthetic Biology

    Tags

    mNeonGreen

    Expression

    Mutation

    3insG

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJethlpAmNeonGreenKm

    Plasmid

    #206816

    Purpose

    Template for hlpA-mneongreen amplification associated with kanamycin resistance gene

    Depositor

    Virginie Libante

    Article

    Mignolet et al bioRxiv 2025.05.10.653270

    Insert

    hlpA
    

    Use

    Synthetic Biology

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Ec dnaN-pHK/P

    Plasmid

    #237239

    Purpose

    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)

    Depositor

    Michael O'Donnell

    Insert

    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    

    Use

    Tags

    his-10+PKA kinase motif+PreScission Protease clea…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQAT37

    Plasmid

    #223409

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYPQAT40

    Plasmid

    #223412

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pYPQAT19

    Plasmid

    #223391

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYPQAT10

    Plasmid

    #223382

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQAT07

    Plasmid

    #223379

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

    Plasmid

    #235150

    Purpose

    Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    HaloTag linked to SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

    Plasmid

    #231372

    Purpose

    Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

    Depositor

    David Bartel

    Article

    Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

    Insert

    Human Argonaute2 (AGO2 Synthetic, Human)
    

    Use

    Tags

    3xFLAG-SUMO_Eu1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGMC00035 (aka pMG0784)

    Plasmid

    #210768

    Purpose

    Lentiviral vector to express human ADRM1

    Depositor

    Raj Chari

    Article

    Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

    Insert

    ADRM1 (ADRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBWB514

    Plasmid

    #209326

    Purpose

    p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.

    Depositor

    Adam Arkin

    Article

    Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tet

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCambia-dLB-kanR-GFP

    Plasmid

    #223177

    Purpose

    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

    Depositor

    Andreas Stuermer

    Type

    Empty backbone

    Use

    Ti plasmid for agrobacterium

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pBES1-LacOx50-blast

    Plasmid

    #210221

    Purpose

    Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBEL2319

    Plasmid

    #195697

    Purpose

    Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    Foldon-MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

    Plasmid

    #178991

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pEBTetD-SNAP-Cep27

    Plasmid

    #136809

    Purpose

    Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep27 (HAUS2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. rs214-minor

    Plasmid

    #113380

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2149943 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. pFA6a-mCherry-hphMX6

    Plasmid

    #105156

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    mCherry-hphMX6
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCS2-Cas9

    Plasmid

    #47322

    Purpose

    For in vivo expression of Cas9 or generation of Cas9 mRNA

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR; Xenopus and zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLenti6.3-OGDH_wobble

    Plasmid

    #110938

    Purpose

    OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    OGDH wobble cDNA (OGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    15 silent wobble mutations corresponding to three…

    Promoter

    SV40

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLNCX2-CFP-VGPDFGR-YFP

    Plasmid

    #59587

    Purpose

    Encodes a granzyme B FRET reporter

    Depositor

    Tim Mitchison

    Article

    Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

    Insert

    ECFP-VGPDFGR-Venus
    

    Use

    Retroviral

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRSFDuet-1_VNp-DNAseI

    Plasmid

    #182409

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. GST-RH▲-MNase

    Plasmid

    #136292

    Purpose

    Expression of catalytic inactive RNase H fused to micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    RNase HI-D10R-E48R and MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge