165,567 results

1. pAAV-hSyn-GRAB-rDA3h

   Plasmid

   #208703

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHREAC

   Plasmid

   #134908

   Purpose

   Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

   Depositor

   George Lomonossoff

   Article

   Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   double 35S

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSicoR-eIF1a-GFP-RPL10a

   Plasmid

   #159890

   Purpose

   Expresses GFP-RPL10a for ribosomal immunoprecipitation

   Depositor

   James Ellis

   Article

   Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

   Insert

   RPL10a (RPL10A Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Promoter

   eIF1a

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIB

   Plasmid

   #80610

   Purpose

   pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nb-gp41-Halo (MoonTag-Nb-Halo)

   Plasmid

   #128603

   Purpose

   Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   Nanobody_gp41
   

   Use

   Lentiviral

   Tags

   GB1 and Halo

   Promoter

   SFFV

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TRE-dCas9-Zim3-MeCP2

   Plasmid

   #220143

   Purpose

   doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

   Depositor

   Frank Buchholz

   Article

   Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

   Insert

   dCas9-Zim3-MeCP2
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   Tre-3G

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-Sec16A

   Plasmid

   #36155

   Purpose

   Expresses Sec16A as a GFP fusion

   Depositor

   David Stephens

   Article

   Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

   Insert

   Sec16A (SEC16A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPGK-T7/2-CD44s (fl)

   Plasmid

   #137812

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44s (fl) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length, I186T and I253V on CD44

   Promoter

   PGK

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV HaloTag-LgBiT Vector

    Plasmid

    #236917

    Purpose

    Mammalian expression vector encoding HaloTag(R)-LgBiT for use in HiBiT cell lines

    Depositor

    Promega Corporation

    Has Service

    DNA

    Type

    Empty backbone

    Tags

    HaloTag (R)-LgBiT

    Expression

    Mammalian

    Mutation

    None

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAGM4723

    Plasmid

    #48015

    Purpose

    Please see depositor comments section.

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. GBX

    Plasmid

    #64123

    Purpose

    a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

    Depositor

    Linzhao Cheng

    Article

    Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

    Insert

    GFP-2A-BCL2L1 (BCL2 Human)
    

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-CreERT2

    Plasmid

    #139547

    Purpose

    CreERT2 ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    CreERT2
    

    Use

    Cre/Lox

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pc DNA FRT TO- APPSwed/Ind

    Plasmid

    #114194

    Purpose

    Creation of a stable cell line expressing APP mutation (AD in vitro study)

    Depositor

    Aleksandra Radenovic

    Insert

    Amyloid-Precursor-Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    Popular mouse model, overexpress 2 mutations: Swe…

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. FKBP-mCherry

    Plasmid

    #108122

    Purpose

    Recruitable fluorophore

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHeACT-mEGFP-NLS

    Plasmid

    #205003

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Casp3-myc

    Plasmid

    #11813

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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