166,224 results

1. Tol2-ngn1-Trpa1b-2A-mCherry

   Plasmid

   #106425

   Purpose

   For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

   Depositor

   Randall Peterson

   Article

   Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

   Inserts

   cmlc2 promoter
   Trpa1b (trpa1b Zebrafish)
   

   Use

   Gateway

   Tags

   2A-mCherry

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pFEndoNA2

   Plasmid

   #174667

   Purpose

   Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA2 fusion protein
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

   Plasmid

   #205766

   Purpose

   Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-NUP50-N-10

   Plasmid

   #55111

   Purpose

   Localization: Nuclear Pore, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   NUP50 (NUP50 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-TfR-mApple

   Plasmid

   #179384

   Purpose

   Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

   Depositor

   Angus Johnston

   Article

   Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

   Insert

   Transferrin receptor 1 - mApple (TFRC Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   mApple

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCM66T: trimmed pCM66 backbone

   Plasmid

   #74738

   Purpose

   Small IncP-based broad host range replicating vector

   Depositor

   Mary Lidstrom

   Type

   Empty backbone

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

   Plasmid

   #71707

   Purpose

   A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

   Depositor

   Giulio Draetta

   Article

   Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

   Insert

   humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
   

   Use

   CRISPR

   Tags

   3xFLAG and hGEM(1/110)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-centrin-1

   Plasmid

   #72641

   Purpose

   The plasmid encodes centrin-1 with an N-terminal EGFP tag

   Depositor

   Michel Bornens

   Insert

   centrin-1 (CETN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLOX-CWBmi1

   Plasmid

   #12240

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   Bmi1 (Bmi1 Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   CMV promoter upstream of mBmi-1. LoxP in 3' …

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLOX-CW-CRE

    Plasmid

    #12238

    Depositor

    Didier Trono

    Article

    Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

    Insert

    CRE
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. Src-EGFP

    Plasmid

    #110496

    Purpose

    Fluorescent Src

    Depositor

    Terence Dermody, Bernardo Mainou

    Article

    Mainou et al J Virol. 2011 Apr;85(7):3203-13. doi: 10

    Insert

    c-Src (Src Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-gfaABC1D-R-iLACCO1.2

    Plasmid

    #208104

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1
    

    Use

    AAV

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTOX8

    Plasmid

    #127453

    Purpose

    Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Inserts

    mqsR toxin
    tsPurple chromoprotein
    

    Expression

    Bacterial

    Mutation

    Codon optimized from protein sequence in FPbase

    Promoter

    apFAB46 and pRha

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

    Plasmid

    #223273

    Purpose

    Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. ART

    Plasmid

    #214063

    Purpose

    Fluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-ABCD3-RFP-Puro

    Plasmid

    #170168

    Purpose

    Mammalian Expression of Ef1a-ABCD3-RFP-Puro

    Depositor

    Christopher Moxham

    Insert

    ABCD3-RFP-Puro
    

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. RepX_pET28a(+)

    Plasmid

    #172199

    Purpose

    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

    Depositor

    Taekjip Ha

    Article

    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

    Insert

    RepX superhelicase
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    C18L, C43S, C167V, S400C, C612A

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0741

    Plasmid

    #142216

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    JDP2 (JDP2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-GST-TEV-BRD4(42-459) BD1-BD2

    Plasmid

    #233887

    Purpose

    pGex for protein overexpression of human BRD4

    Depositor

    Alessio Ciulli

    Article

    Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7-p53DD-pcDNA3

    Plasmid

    #25989

    Depositor

    William Kaelin

    Article

    Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

    Insert

    tumor protein p53 (TP53 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    truncated cDNA encodes truncated protein (aa 300-…

    Available Since

    Oct. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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