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8,885 results

Showing: 6951 - 7000 of 8885 results

  4. pBOB-CAG-SARS-CoV2-Spike D614G-HA

    Plasmid
    #158761
    Purpose
    3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Article
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Spike D614G
    Promoter
    CAG
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158761-plasmid-map-sequence-id-309514

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  13. mCherry-ARP2-N-14

    Plasmid
    #54980
    Purpose
    Localization: Actin Related Protein, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ARP2 (ACTR2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. mApple-VE-Cadherin-N-10

    Plasmid
    #54959
    Purpose
    Localization: Tight Junctions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    VE-Cadherin (CDH5 Human)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    I503T
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54959-plasmid-map-sequence-id-209018

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  24. 401 pSG5L

    Plasmid
    #10737
    Depositor
    William Sellers
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Sept. 27, 2005
    Availability
    Academic Institutions and Nonprofits only

  26. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74407-plasmid-map-sequence-id-140317

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  28. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Article
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    96921-plasmid-map-sequence-id-181715

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  38. PB-TAG-ERP2

    Plasmid
    #80479
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80479-plasmid-map-sequence-id-484261

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  40. mTLR9

    Plasmid
    #13091
    Depositor
    Ruslan Medzhitov
    Insert
    TLR9 (Tlr9 Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13091-plasmid-map-sequence-id-142445

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  43. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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Showing: 6951 - 7000 of 8885 results