19,200 results

1. pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE

   Plasmid

   #130999

   Purpose

   Double floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet-Kv2.1

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ezh2[FL]-dCas9

   Plasmid

   #100086

   Purpose

   Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   14056 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[FL]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 gfaABC1D-tdTomato

   Plasmid

   #44332

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al J Gen Physiol. 2013 Apr 15.

   Has Service

   AAV5

   Insert

   tdTomato
   

   Use

   AAV; Adeno-associated virus ()

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304 Luciferase-V5 blast

   Plasmid

   #98580

   Purpose

   Lentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

   Plasmid

   #31482

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce Target) EF Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   mCherry has M9S and M16L mutations. This is what …

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TREtight-mTagBFP2-B19G

   Plasmid

   #100799

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pscALPSpuro-MmACE2 (Mouse)

   Plasmid

   #158087

   Purpose

   Expresses mouse ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Mouse ACE2 (Ace2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. spNW50

   Plasmid

   #173486

   Purpose

   Expresses spNW50 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW50
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Tat HA (P#29)

   Plasmid

   #14654

   Depositor

   Matija Peterlin

   Article

   Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

   Insert

   Tat (tat HIV)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmScarlet_C1

    Plasmid

    #85042

    Purpose

    Mammalian expression of mScarlet

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcU6_3 sgRNA

    Plasmid

    #92359

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.3 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chick U6.3

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SIN40C.SFFV.Luciferase.IRES.GFP

    Plasmid

    #169308

    Purpose

    Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LeGO-mOrange2

    Plasmid

    #85212

    Purpose

    Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    mOrange2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIPZ shSTING-Hsa

    Plasmid

    #128158

    Purpose

    Doxycyclin inducible shRNA knockdown of human STING gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shSTING Hsa (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-GFAP-hM4D(Gi)-mCherry

    Plasmid

    #50479

    Purpose

    Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD33-mf-lon

    Plasmid

    #21867

    Depositor

    Robert Sauer

    Article

    Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

    Insert

    Lon (Mfl404 Mesoplasma florum)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all TGA codons changed to TGG codons

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-HA-HA-humanCMYC

    Plasmid

    #74164

    Purpose

    Mammalian expression of human cMyc

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    cMyc (MYC Human)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR_SFFV_LaG17_synNotch_TetRVP64

    Plasmid

    #79128

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17 GFP nanobody synNotch TetRVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTREtight2

    Plasmid

    #19407

    Depositor

    Markus Ralser

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBV-Luc

    Plasmid

    #16539

    Purpose

    luciferase reporter plasmid with very low basal activity

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTriExRhoA2G

    Plasmid

    #40176

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-Wnt3A

    Plasmid

    #35908

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt3A (WNT3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-mNox4

    Plasmid

    #69353

    Purpose

    expresses mouse Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    mNox4 (Nox4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

26. DNMT3A-dCas9

    Plasmid

    #100090

    Purpose

    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3A-dCas9-NLS

    Expression

    Mammalian

    Mutation

    includes aa 602-912

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pShuttle-CMV

    Plasmid

    #16403

    Purpose

    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV hUbC-Cas9-T2A-GFP

    Plasmid

    #53190

    Purpose

    3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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