We narrowed to 6,558 results for: siae
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Plasmid#167453PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoterDepositorInsertPSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
ExpressionYeastPromoterPSEC63AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Plasmid#74009PurposeTissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoterDepositorInserts5x UAS
Cas9
T2A
GFP
U6 promoter
UseCRISPRAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA
Plasmid#155125PurposeTol2 vector containing Cre-inducible QFGal4-switchDepositorInsertQFGal4-switch
UseCre/Lox; Zebrafish expressionPromoterUbiAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Gm
Plasmid#222335PurposeExpresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3DepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
HDAC6 Flag
Plasmid#13823PurposeMammalian expression of human histone deacetylase 6 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
Plasmid#52889Purpose"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expressionDepositorInsertGal4 (GAL4 Budding Yeast)
ExpressionInsectAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK419 (GAL-OsTIR1(F74G))
Plasmid#140656PurposeGAL-OsTIR1(F74G)DepositorInsertGAL-OsTIR1(F74G)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyExpressionYeastPromoterPGK1, TDH3, TIP1, and ZEO1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-PDI
Plasmid#202486PurposeExpresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
ExpressionBacterialPromoterEM7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
ExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
HDAC7 Flag
Plasmid#13824PurposeMammalian expression of human histone deacetylase 7 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL005635 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-DsbC
Plasmid#202485PurposeExpresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)
ExpressionBacterialPromoterEM7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK036.TRE-Flpe-WPRE (Supernova)
Plasmid#85007PurposeFor Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.DepositorInsertNLS-FLPe-WPRE
ExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED8
Plasmid#15406DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
UG76-pLYS2-GFP-PKCbeta
Plasmid#184762PurposeMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNAP-OMP25
Plasmid#141149PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHLUM
Plasmid#40276DepositorExpressionYeastPromoterendogenous LEU2, endogenous MET15/17, and endogen…Available SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAEL010097-Cas9
Plasmid#100707PurposeThe promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL010097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL010097 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Km
Plasmid#222334PurposeExpresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3DepositorAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-NES
Plasmid#64727PurposeCytosol-targeted, FRET-based PKA activity reporter.DepositorInsertAKAR4-NES
TagsCerulean, NES, and cpVenus[E172]ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-4031NES
Plasmid#105241PurposeA Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.DepositorInsertAMPK-EV
ExpressionMammalianPromoterCAGGSAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDR-eGFP (pXK-1647)
Plasmid#208824PurposeThe HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDRDepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIB1
Plasmid#28245PurposeExpresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activationDepositorAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK425 (GAL-OsTIR1(F74A))
Plasmid#140657PurposeGAL-OsTIR1(F74A)DepositorInsertGAL-OsTIR1(F74A)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTEF1
Plasmid#46922PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoterDepositorAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pScRad52-SpCas9 (pXK-1646)
Plasmid#208823PurposeCRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.DepositorTypeEmpty backboneUseCRISPR; Cas9TagsRad52ExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-TsCC(B)::KANMX
Plasmid#176397PurposePlasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.DepositorInsertmScarlet-TsCC(B)
TagsmScarlet fused to TsCC(B)ExpressionBacterial and YeastPromoternoneAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB_tetO7_sfGFP
Plasmid#196616PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeastDepositorInsertSuperfolder GFP
ExpressionYeastPromotertetO7 promoterAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
yHS1-M7A
Plasmid#159151PurposeCytosolic labile heme reporterDepositorInsertyHS1-M7A
ExpressionYeastMutationMutated Met 7 of the cyt b562 module of yHS1 to A…PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
720-2µ-maxHIS3
Plasmid#40612DepositorInsertHIS3 (HIS3 Synthetic, Budding Yeast)
TagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3Available SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 wt (Nigg RG6)
Plasmid#41160DepositorAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTW040
Plasmid#64769PurposeFor integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminatorDepositorInsertCre-EBD78
Tagsfused to the human Estrogen Binding Domain (EBD),…ExpressionYeastPromoterTDH3Available SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRExpressionBacterial and YeastAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSS146
Plasmid#64770PurposeFor integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminatorDepositorInsertCre-EBD78
Tagsfused to the human Estrogen Binding Domain (EBD),…ExpressionYeastPromoterTDH4Available SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNES-EGFP-C1-flex-PABD-Spo20
Plasmid#220081PurposeSpo20-PABD PA biosensor with a flexible linker to aid in nuclear exportDepositorInsertSpo20 (SPO20 Budding Yeast)
TagsX. leavis map2k1.L(32-44)-EGFP-SGGGSGGSExpressionMammalianMutationamino acids 51-91PromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only