Skip to main content
Addgene
Showing: 5641 - 5680 of 8614 results

  3. mCherry-ARP2-N-14

    Plasmid
    #54980
    Purpose
    Localization: Actin Related Protein, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ARP2 (ACTR2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Tags
    Expression
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

    Enlarge

  12. pBMN-I-GFP

    Plasmid
    #1736
    Purpose
    This retroviral vector has an IRES-GFP downstream of the cloning sites
    Depositor
    Garry Nolan
    Insert
    IRES-GFP
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    1736-plasmid-map-sequence-id-418359

    Enlarge

  13. AAVS1-idCas9-vpr

    Plasmid
    #89985
    Purpose
    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus
    Depositor
    Jie Na
    Article
    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/
    Insert
    dCas9-VPR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    89985-plasmid-map-sequence-id-180638

    Enlarge

  19. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

    Enlarge

  28. pcDNA-GFP-STAT1

    Plasmid
    #11987
    Depositor
    Steven Johnson
    Insert
    Signal transducer and activator of transcription 1 (STAT1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    no mutations
    Promoter
    Available since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only

  34. pYLCRISPR/Cas9P35S-B

    Plasmid
    #66190
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Use
    Tags
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66190-plasmid-map-sequence-id-169495

    Enlarge

  35. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive native promoters
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65006-plasmid-map-sequence-id-109903

    Enlarge

Showing: 5641 - 5680 of 8614 results