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177,170 results

Showing: 7101 - 7150 of 177170 results

  1. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  5. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid
    #177672
    Purpose
    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells
    Depositor
    Karl Deisseroth
    Has service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    nEF
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    177672-plasmid-map-sequence-id-348533

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  7. pSL1777 (pEffector)

    Plasmid
    #160731
    Purpose
    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins and crRNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    160731-plasmid-map-sequence-id-318598

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  8. pBOB-CAG-SARS-CoV2-Spike D614G-HA

    Plasmid
    #158761
    Purpose
    3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Publication
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Spike D614G
    Promoter
    CAG
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    158761-plasmid-map-sequence-id-309514

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  9. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has service
    AAV8 and AAV Retrograde
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    154868-plasmid-map-sequence-id-308827

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  12. pCAG-All-in-one-hCascade

    Plasmid
    #134919
    Purpose
    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    134919-plasmid-map-sequence-id-272938

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  13. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Publication
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MYC (MYC Human)
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    129776-plasmid-map-sequence-id-263368

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  14. MLRV

    Plasmid
    #118069
    Purpose
    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Promoter
    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    118069-plasmid-map-sequence-id-240503

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  18. CDH5-AbR-GFP

    Plasmid
    #122970
    Purpose
    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.
    Depositor
    Budd A. Tucker
    Inserts
    CDH5p
    CopGFP
    Zeocin
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    NA
    Available since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    122970-plasmid-map-sequence-id-244602

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  20. GATA3-pCDNA3.1

    Plasmid
    #115271
    Purpose
    Expresses human GATA3 in mammalian cells
    Depositor
    Arne S. Schaefer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GATA3 (GATA3 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    115271-plasmid-map-sequence-id-220672

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  26. VEGFR2-mCh

    Plasmid
    #108854
    Purpose
    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker
    Depositor
    Kalina Hristova
    Publication
    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KDR (KDR Human)
    Tags
    Labeled with mCherry on the C-Terminus via a 3 am…
    Expression
    Mammalian
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    108854-plasmid-map-sequence-id-208010

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  32. pMH-SFB

    Plasmid
    #99391
    Purpose
    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)
    Depositor
    Michael Huen
    Publication
    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Type
    Empty backbone
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Available since
    Sept. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    99391-plasmid-map-sequence-id-194202

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  34. pAAV-i53

    Plasmid
    #92170
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Publication
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92170-plasmid-map-sequence-id-182617

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  36. pCMV-HF-BE3

    Plasmid
    #87439
    Purpose
    Expresses HF-BE3 with C-terminal NLS in mammalian cells
    Depositor
    David Liu
    Publication
    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HF-BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)
    Expression
    Mammalian
    Mutation
    Mammalian codon-optimized Cas9-HF
    Promoter
    CMV
    Available since
    June 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    87439-plasmid-map-sequence-id-298693

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  41. AICSDP-7:SEC61B-mEGFP

    Plasmid
    #87426
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    87426-plasmid-map-sequence-id-173967

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  44. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Publication
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InsRA (INSR Human)
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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  45. pKHR8

    Plasmid
    #74625
    Purpose
    a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.
    Depositor
    David Grunwald
    Publication
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    cmlc2::mCherry
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    alpha-crystallin and cmlc2
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    74625-plasmid-map-sequence-id-200444

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  49. pET28a SnoopTag-MBP

    Plasmid
    #72323
    Purpose
    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
    Depositor
    Mark Howarth
    Publication
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-MBP
    Tags
    N-terminal His6 tag. MBP is fused at the C-termin…
    Expression
    Bacterial
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    72323-plasmid-map-sequence-id-137256

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  50. pAAV-CaMKII-GFP

    Plasmid
    #64545
    Purpose
    AAV-mediated expression of GFP under the CaMKII promoter.
    Depositor
    Edward Boyden
    Insert
    GFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CaMKII
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    64545-plasmid-map-sequence-id-130468

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Showing: 7101 - 7150 of 177170 results