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We narrowed to 16,889 results for: Por

Showing: 7401 - 7450 of 16889 results
  1. pEasyG2_hph

    Plasmid
    #184915
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.
    Depositor
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pGGD_MiniTurboID-YFP

    Plasmid
    #222434
    Purpose
    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.
    Depositor
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. NL4-3 mCherry Luciferase

    Plasmid
    #44965
    Purpose
    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
    Depositor
    Insert
    mCherry, Luciferase, IRES
    Use
    Lentiviral
    Tags
    Luciferase, IRES and mCherry with D212G
    Expression
    Mutation
    S34C mutation in Nef
    Promoter
    Available Since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

    Plasmid
    #205766
    Purpose
    Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells
    Depositor
    Insert
    JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pGL3-NBRE3

    Plasmid
    #208254
    Purpose
    pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells
    Depositor
    Insert
    3X NBRE-LUC
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40 early promoter
    Available Since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pBpaRS3tRNA

    Plasmid
    #197102
    Purpose
    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.
    Depositor
    Insert
    TyrRS variant, amber suppressor tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)
    Promoter
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pG3H-PE3max-attR1R2

    Plasmid
    #213049
    Purpose
    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
    Depositor
    Inserts
    Cas9 nickase
    M-MLV Reverse Transcriptase
    Use
    Note: this plasmid needs helper pvs1-vir2 for rep…
    Tags
    Expression
    Plant
    Mutation
    H840A, R240K, N413K,
    Promoter
    Available Since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pGGB_YFP-MiniTurboID

    Plasmid
    #222433
    Purpose
    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.
    Depositor
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid
    #89873
    Purpose
    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production
    Depositor
    Insert
    bArrestin2-TevC-P2A-TdTomato
    Use
    AAV
    Tags
    TdTomato
    Expression
    Mammalian
    Mutation
    R173H in TdTomato (Please see depositor comments …
    Promoter
    Human synapsin
    Available Since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pAL113

    Plasmid
    #22471
    Purpose
    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.
    Depositor
    Insert
    PhyB NT (PHYB Mustard Weed, Mouse)
    Use
    Synthetic Biology
    Tags
    10aaLinker-mCFP-HrasCT
    Expression
    Mammalian
    Mutation
    Y276H
    Promoter
    CMV
    Available Since
    Nov. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
  11. SBP-GFP-Tac-TC

    Plasmid
    #162506
    Purpose
    Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells
    Depositor
    Insert
    GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 7401 - 7450 of 16889 results