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171,009 results

Showing: 7501 - 7550 of 171009 results

  5. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

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  8. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid
    #192552
    Purpose
    Expresses nuclear localized tdTomato under CAG promoter
    Depositor
    Guoping Feng
    Has Service
    AAV PHP.eB and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    NLS
    Mutation
    deleted E273 and G274 compared to FPbase ID: PGG5S
    Promoter
    CAG
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192552-plasmid-map-sequence-id-403193

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  14. tet-pLKO.puro_shGFP

    Plasmid
    #110421
    Purpose
    shGFP control, silence GFP gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GFP
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110421-plasmid-map-sequence-id-213312

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  15. Venus-tBid-4E-pEGFP-C1

    Plasmid
    #166743
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    tBid-4E (Bid Mouse)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166743-plasmid-map-sequence-id-329004

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  20. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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  34. CHRM5-DuET

    Plasmid
    #213211
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM5 (CHRM5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213211-plasmid-map-sequence-id-430773

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  37. GSK3 beta pGEX

    Plasmid
    #15898
    Depositor
    Jim Woodgett
    Insert
    GSK3 beta (GSK3B Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Nov. 1, 2007
    Availability
    Industry, Academic Institutions, and Nonprofits
    15898-plasmid-map-sequence-id-413253

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  38. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-490369

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  39. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

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  42. mRuby-Mito-7

    Plasmid
    #55874
    Purpose
    Localization: Mitochondria, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Tags
    mRuby
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7501 - 7550 of 171009 results