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Addgene

We narrowed to 34,448 results for: CaS

Showing: 7501 - 7550 of 34448 results
  1. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid
    #176139
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro

    Plasmid
    #176089
    Purpose
    EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette
    Depositor
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutations in Lys35 to Ala, Lys68 to Ala, and Lys7…
    Promoter
    CMV
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pICSL1P6_AtU626

    Plasmid
    #86192
    Purpose
    U626 Guide acceptor
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pICSL1P5_AtU626

    Plasmid
    #86191
    Purpose
    U626 Guide acceptor
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pICSL1P4_AtU626

    Plasmid
    #86190
    Purpose
    U626 Guide acceptor
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pICSL1P1_AtU626

    Plasmid
    #86187
    Purpose
    U626 Guide acceptor
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pICSL1P2_AtU626

    Plasmid
    #86188
    Purpose
    U626 Guide acceptor
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pICSL1P7_AtU626

    Plasmid
    #86193
    Purpose
    U626 Guide acceptor
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pICSL1P3_AtU626

    Plasmid
    #86189
    Purpose
    U626 Guide acceptor
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pCBC-DT2T3

    Plasmid
    #50591
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  13. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  14. pPP085

    Plasmid
    #158795
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Insert
    CasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. pCBC-DT3T4

    Plasmid
    #50592
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  16. pAL204

    Plasmid
    #22282
    Purpose
    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
    Depositor
    Insert
    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2010
    Availability
    Academic Institutions and Nonprofits only
Showing: 7501 - 7550 of 34448 results