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We narrowed to 18,759 results for: nls

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Showing: 6161 - 6200 of 18759 results

  3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

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  4. TBG-Vector

    Plasmid
    #109396
    Purpose
    AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver
    Depositor
    Sidi Chen
    Publication
    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:
    Type
    Empty backbone
    Use
    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…
    Expression
    Mammalian
    Promoter
    TBG
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109396-plasmid-map-sequence-id-222776

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  9. pDIRECT_26H

    Plasmid
    #91150
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91150-plasmid-map-sequence-id-182284

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  11. pJH2970

    Plasmid
    #100954
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker
    Depositor
    James Haber
    Publication
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100954-plasmid-map-sequence-id-193247

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  12. pHD157

    Plasmid
    #84033
    Purpose
    Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish
    Depositor
    Daniel Hesselson, Didier Stainier
    Publication
    Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10
    Inserts
    Cre
    Venus
    Use
    Cre/Lox; Zebrafish transgenesis
    Tags
    mCherry
    Promoter
    Crystalin and fabp10
    Available since
    Nov. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    84033-plasmid-map-sequence-id-166816

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  15. pZ8-P_dCas9

    Plasmid
    #74063
    Purpose
    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
    Depositor
    Timothy Lu
    Publication
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Propionate inducible promoter (prp)
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74063-plasmid-map-sequence-id-141970

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  16. pAdx-CMV-iCre-P2A-tdTomato

    Plasmid
    #73351
    Purpose
    Express iCre and tdTomato under CMV promoter
    Depositor
    Kazuhiro Oka
    Insert
    CMV-iCre-tdtomato
    Use
    Adenoviral
    Promoter
    CMV
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73351-plasmid-map-sequence-id-135084

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  18. pCDH-CB-copGFP-T2A-iCre

    Plasmid
    #72256
    Purpose
    Express copGFP and iCre under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-iCre
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72256-plasmid-map-sequence-id-134470

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  22. pJZC33

    Plasmid
    #62330
    Purpose
    sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Publication
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2 binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA
    Promoter
    CMV and U6
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62330-plasmid-map-sequence-id-102252

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  25. pMB67

    Plasmid
    #47947
    Purpose
    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Publication
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and egl-13 NLS
    Expression
    Worm
    Promoter
    hsp-16.48
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    47947-plasmid-map-sequence-id-68808

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  26. lentiCRISPR - EGFP sgRNA 4

    Plasmid
    #51763
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51763-plasmid-map-sequence-id-79382

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  27. pCMV_dCas9_VP64

    Plasmid
    #49015
    Purpose
    encodes human-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Publication
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9_VP64 (human-codon-optimized)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49015-plasmid-map-sequence-id-79086

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  37. pCAG_PCP-T5Exo

    Plasmid
    #214736
    Purpose
    encodes T5 exonuclease with a PP7 coat protein fused to the N terminus
    Depositor
    Gil Westmeyer
    Publication
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG_PCP-T5Exo_bGH
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214736-plasmid-map-sequence-id-424997

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  39. ABE8r

    Plasmid
    #208294
    Purpose
    Expresses ABE8r in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA8r-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208294-plasmid-map-sequence-id-410370

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  40. pX330-PITCh-LMNB1

    Plasmid
    #207770
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207770-plasmid-map-sequence-id-416199

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Showing: 6161 - 6200 of 18759 results