8,803 results

1. pET28a-sfGFP

   Plasmid

   #85492

   Purpose

   pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFREE

   Plasmid

   #92050

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pRhamBAD and ptet

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV_BE4max_P2A_GFP

   Plasmid

   #112099

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   BE4max_P2A_GFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ph7SK-gRNA

   Plasmid

   #53189

   Purpose

   Expresses the S. pyogenes sgRNA from the 7SK promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human 7SK

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CaMKIIa-hM3D(Gq)-mCherry

   Plasmid

   #50476

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

   Plasmid

   #125712

   Purpose

   Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   eOPN3
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   short version of αCamKinase II promoter (0.4kb)

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lenti MS2-P65-HSF1_Hygro

   Plasmid

   #61426

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Insert

   MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1A

   Available Since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDY1173_ADAR1p150

   Plasmid

   #193192

   Purpose

   Express full length ADAR1p150

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   ADAR1p150 (ADAR Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

   Plasmid

   #62723

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1 and AAV5

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GABA(A) receptor subunit a1SE

    Plasmid

    #49168

    Purpose

    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Tija Jacob, Stephen Moss

    Article

    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

    Insert

    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    

    Tags

    myc and pHGFP (pHIuorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TETv4

    Plasmid

    #167983

    Purpose

    Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

    Depositor

    Luke Gilbert

    Article

    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

    Insert

    TETv4 (TET1CD-XTEN80-dCas9)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    3xNLS, TET1 catalytic domain, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmiRFP670-N1

    Plasmid

    #79987

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    miRFP670
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-CFP

    Plasmid

    #13030

    Purpose

    Mammalian expression vector for cloning your gene fused to CFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-GfaABC1D-Cre-4x6T

    Plasmid

    #196410

    Purpose

    Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Has Service

    AAV5

    Insert

    Cre
    

    Use

    AAV; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAVS1 pegRNA1 with trimmed attP

    Plasmid

    #222346

    Purpose

    pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA1
    

    Use

    CRISPR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-evoCAST-evoTnsAB

    Plasmid

    #234729

    Purpose

    Evolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    evoPseTnsAB
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAVS1 pegRNA2 with trimmed attP

    Plasmid

    #222347

    Purpose

    pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA2
    

    Use

    CRISPR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1GFP/RNase H1 D210N

    Plasmid

    #174448

    Purpose

    Express GFP-tagged RNase H1 D210N in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    D210N

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-FLAG-Ku80

    Plasmid

    #46958

    Purpose

    Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku80 (XRCC5 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge