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pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

Plasmid

#111568

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Has Service

AAV5

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

AAV

Tags

mCherry

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-ABERA-P2A-Puro

Plasmid

#112675

Purpose

Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

ABERA

Use

Lentiviral

Tags

FLAG

Mutation

D10A

Promoter

EF1s

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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PresentER-SIINFEKL (mCherry)

Plasmid

#102945

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Chicken, Synthetic)

Use

Retroviral

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only
pAAV.hSyn.eGFP.WPRE.bGH

Plasmid

#105539

Purpose

AAV expression of EGFP from hSyn promoter

Depositor

James M. Wilson

Has Service

AAV1

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.GCaMP6s.WPRE.SV40

Plasmid

#100843

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Ezh2[FL]-dCas9

Plasmid

#100086

Purpose

Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

14056 (Ezh2 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Ezh2[FL]-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-NLS-mRuby2

Plasmid

#99130

Purpose

An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

NLS-mRuby2

Use

AAV

Expression

Mammalian

Promoter

mDLX5/6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEff

Plasmid

#97423

Purpose

Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

Depositor

Nikolai Ravin

Article

Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS BFP

Plasmid

#91757

Purpose

Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Blue Fluorescent Protein

Tags

Hexa-Histidine, Xpress epitope for detection with…

Expression

Bacterial

Mutation

GFP (Y66H)

Promoter

T7

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Lb

Plasmid

#86197

Purpose

LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

gRNA cloning site

Use

CRISPR

Promoter

Maize ubiquitin 1

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ179

Plasmid

#85996

Purpose

One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified bovine U6-2

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Promoter

None

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-GFP-U6-gRNA

Plasmid

#79144

Purpose

All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

Depositor

Jizhong Zou

Article

Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

Insert

SpCas9

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-PGK

Plasmid

#72268

Purpose

Express gene of interest under PGK (phosphoglycerate kinase I) promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA Flag Lamp2b-HA

Plasmid

#71292

Purpose

Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

Flag, HA, and Lamp2 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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NPY-mCherry

Plasmid

#67156

Purpose

Expresses NPY-mCherry

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

NPYmCherry (NPY Human)

Tags

mCherry

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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GFP_CD47_LU

Plasmid

#65474

Purpose

GFP tagged CD47 with its own long 3'UTR

Depositor

Christine Mayr

Article

Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

Inserts

Signal peptide of CD47
GFP
CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).

Expression

Mammalian

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BRD3

Plasmid

#65377

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD3 (BRD3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSR58.6

Plasmid

#63176

Purpose

Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Tags

His6-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgG4/κ

Plasmid

#61887

Purpose

Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBMN-mCherry-Parkin

Plasmid

#59419

Purpose

to make mCherry-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

mCherry-Parkin (PRKN Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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p201N Cas9

Plasmid

#59175

Purpose

Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
nptII

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-ARP2-N-14

Plasmid

#54980

Purpose

Localization: Actin Related Protein, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ARP2 (ACTR2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
AAV pmSyn1-EBFP-Cre

Plasmid

#51507

Purpose

Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV5

Insert

EBFP-Cre

Use

AAV

Promoter

pmSyn1

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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DRH313: FCK-CheRiff-eGFP

Plasmid

#51693

Purpose

Channelrhodopsin with improved sensitivity and minimal red light photocurrent

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

CheRiff-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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