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159,557 results

Showing: 8651 - 8700 of 159557 results

  1. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid
    #230026
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Turbo-ID, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230026-plasmid-map-sequence-id-457126

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  5. pLX302 EGFP-V5

    Plasmid
    #141348
    Purpose
    Expresses V5 tagged GFP
    Depositor
    Kevin Janes
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    V5 tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    141348-plasmid-map-sequence-id-307212

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  6. pCM66T: trimmed pCM66 backbone

    Plasmid
    #74738
    Purpose
    Small IncP-based broad host range replicating vector
    Depositor
    Mary Lidstrom
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74738-plasmid-map-sequence-id-141453

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  12. TFORF2474

    Plasmid
    #144029
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ONECUT3 (ONECUT3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144029-plasmid-map-sequence-id-408964

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  14. pJB06

    Plasmid
    #190480
    Purpose
    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Cas9
    XylR
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Tags
    Expression
    Mutation
    Promoter
    xylR-driven promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    190480-plasmid-map-sequence-id-374623

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  15. pJB07

    Plasmid
    #190481
    Purpose
    Targeting plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Upstream & Downstream pyrE deletion region
    gRNA
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190481-plasmid-map-sequence-id-372027

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  18. TFORF1884

    Plasmid
    #143984
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZIC2 (ZIC2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143984-plasmid-map-sequence-id-400555

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  22. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Promoter
    Available since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  25. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-260007

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  33. pcDNA3-Flag-Tet1 CD Mut

    Plasmid
    #72218
    Purpose
    Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 1 (Tet1 Mouse)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET1 catalytic domain (cata…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  35. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Stacey Edwards
    Article
    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212196-plasmid-map-sequence-id-420116

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  36. C1-MPAct-mCherry

    Plasmid
    #155222
    Purpose
    Monitor changes in the density of membrane-proximal F-actin (MPA)
    Depositor
    Tobias Meyer
    Article
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Insert
    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155222-plasmid-map-sequence-id-310131

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  37. TFORF0060

    Plasmid
    #141501
    Purpose
    Lentiviral vector for overexpressing the CTBP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CTBP2 (CTBP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    141501-plasmid-map-sequence-id-454896

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  41. AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044)

    Plasmid
    #191706
    Purpose
    AI plasmid ALIAS: AiP13044. Optimized expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    191706-plasmid-map-sequence-id-379462

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  42. pLENTI_hACE2_PURO

    Plasmid
    #155295
    Purpose
    Lentiviral vector to generate hACE2 stable expressing cell line
    Depositor
    Raffaele De Francesco
    Insert
    hACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    C9
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155295-plasmid-map-sequence-id-307855

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  47. pCAGIG

    Plasmid
    #11159
    Purpose
    Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10
    Has Service
    Cloning Grade DNA
    Insert
    IRES-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2006
    Availability
    Academic Institutions and Nonprofits only
    11159-plasmid-map-sequence-id-275611

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  50. pRL528

    Plasmid
    #58495
    Purpose
    helper plasmid for bacterial conjugal DNA transfer
    Depositor
    Peter Wolk
    Article
    Elhai et al Methods Enzymol. 1988;167:747-54.
    Insert
    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    58495-plasmid-map-sequence-id-107317

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Showing: 8651 - 8700 of 159557 results