We narrowed to 167,150 results for: Gene

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pCAG-YTB

Plasmid

#26721

Depositor

Edward Callaway

Article

Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

Insert

venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Rabies virus (strain SAD B19), Chicken)

Expression

Mammalian

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAL120-TK

Plasmid

#22053

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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L1_HyR-Ck1

Plasmid

#136125

Purpose

L1 in position 1 (next to LB in L2 construct), for HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:hptII_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pETSXM2-pLacI-mtrC

Plasmid

#174615

Purpose

Bacterial expression of mtrC from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

MtrC

Use

Synthetic Biology; Shuttle vector in e. coli and …

Expression

Bacterial

Promoter

pLacI

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSP1E2D1

Plasmid

#161606

Purpose

Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E2D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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L1_CsR-Ck1

Plasmid

#136124

Purpose

L1 in position 1 (next to LB in L2 construct), for CsR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:mALS_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MX1_pCSdest

Plasmid

#53951

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MX1 (MX1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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Bxb1-GT-CAG-mStayGold

Plasmid

#241387

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CAG promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV4-p100

Plasmid

#23287

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

NFkB p100 subunit (NFKB2 Human)

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_hsNotch1 LNR

Plasmid

#188336

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ14107 fwYellow-targeting sgRNA

Plasmid

#239456

Purpose

guide for fwYellow

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting fwYellow

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ14112 tyrosinase-targeting sgRNA

Plasmid

#239751

Purpose

guide for tyrosinase

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting tyrosinase

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ14103 aeBlue-targeting_sgRNA

Plasmid

#239750

Purpose

guide for aeBlue

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting aeBlue

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-393

Plasmid

#195473

Purpose

Lentiviral Integratable Inducible super-IL2 cytokine + synZiFTR10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF10 BS - ybTATA - IL12] - pSFFV - ZF10 - NS3 - p65

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP8923

Plasmid

#220904

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMDH

Plasmid

#133129

Purpose

A recombinant plasmid expressing mdh (malate dehydrogenase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

malate dehydrogenase gene

Expression

Bacterial

Promoter

malate dehydrogenase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARα.hRXRα

Plasmid

#135411

Purpose

Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-alpha
Human retinoid X receptor-alpha

Expression

Mammalian

Promoter

CMV and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pX459-SpCas9-NG

Plasmid

#171370

Purpose

SpCas9-NG-mediated genome editing.

Depositor

Masahito Ikawa

Article

Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 for sgRNA; CBh for Cas9-2A-puroR

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA9-Halo

Plasmid

#175332

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA9 (HSPA9 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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SAP145

Plasmid

#11239

Depositor

Robin Reed

Article

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Expression

Bacterial

Mutation

First 7 amino acids are missing compared to NP_00…

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_Divergent Donor

Plasmid

#229784

Purpose

Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti GFP-AMOT p80

Plasmid

#32830

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p80 (AMOT Human)

Use

Lentiviral

Tags

GFP-AMOT

Expression

Mammalian

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only
pCk4_spacer

Plasmid

#136074

Purpose

Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM4723:TpCC_Urease

Plasmid

#85982

Purpose

Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Inserts

Cas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2

Use

CRISPR; Diatom (t. pseudonana)

Tags

NLS and YFP

Promoter

FCP and U6

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBP-J23100

Plasmid

#72963

Purpose

Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

J23100 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WT PBRM1- TRIPZ-neo

Plasmid

#107406

Purpose

Mammalian expression, inducible PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-basP:fLuc-TK:rLuc

Plasmid

#138369

Purpose

Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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MSGV Mouse Acceptor

Plasmid

#128176

Purpose

A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Promoter

MSCV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Stat2 pBluescript SK (-)

Plasmid

#8705

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

Stat2 (STAT2 Human)

Expression

Bacterial

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-DEST(f)

Plasmid

#190226

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
U6-AsCas12f-sgRNA-v2

Plasmid

#204639

Purpose

Expression of trancated AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

trancated AsCas12f sgRNA (sgRNA-v2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-16

Plasmid

#160716

Purpose

T-nos Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

nopaline synthase terminator (T-nos) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLTR-Rabies PV

Plasmid

#190897

Purpose

Pseudotyping of lentiviral vectors

Depositor

Jakob Reiser

Article

Federici et al Genet Vaccines Ther. 2009 Jan 13;7:1. do

Insert

Lyssavirus rabies glycoprotein

Expression

Mammalian

Promoter

HIV-1 LTR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Cerulean_Nanotag_70_neg_Control

Plasmid

#130568

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-MyoD

Plasmid

#8398

Depositor

Andrew Lassar

Article

Skapek et al Mol Cell Biol 1996 Dec;16(12):7043-53.

Insert

MyoD (Myod1 Mouse)

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-full-length-sgRNA

Plasmid

#204638

Purpose

Expression of full-length AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

full-length AsCas12f sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-sgRNA (Sp)

Plasmid

#80427

Purpose

U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SB52

Plasmid

#21487

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
pMXs-C16orf80-flag

Plasmid

#70645

Purpose

Expresses C16orf80 in mammalian cells with FLAG-tag epitope.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C16orf80 (CFAP20 Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TL1 DdCBE A1 (DddA6-C)

Plasmid

#221992

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: T1413I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TL1 DdCBE T2 (DddA6-N)

Plasmid

#221993

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: Q1310R, S1330I, T1380I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK7-FLAG-TSC2

Plasmid

#8996

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC2 (TSC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pKAd5f11p-EPG

Plasmid

#122697

Purpose

To generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Insert

GFP

Use

Adenoviral

Promoter

EF1a

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p-LCN2

Plasmid

#28225

Depositor

Sebastien Jauliac

Article

Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

Insert

LCN2 promoter (LCN2 Human)

Use

Luciferase

Promoter

LCN2

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pCk2_spacer

Plasmid

#136072

Purpose

Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLJC6-NADK-3xFLAG

Plasmid

#163452

Purpose

lentiviral overexpression vector for human NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-CMV-GFP-BGHpA

Plasmid

#190239

Purpose

Expresses EGFP in mammalian cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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