19,218 results

1. pLentiCRISPRv2 sgIRF3#5

   Plasmid

   #127642

   Purpose

   Knock-out of human IRF3

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   IRF3 sgRNA (IRF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIW601-KmCRISPR

   Plasmid

   #98907

   Purpose

   K. marxianus CRISPR Plasmid for sgRNA cloning

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBbsr2-4031NES

   Plasmid

   #105241

   Purpose

   A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

   Depositor

   Michiyuki Matsuda

   Article

   Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

   Insert

   AMPK-EV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_smFP V5

   Plasmid

   #59758

   Purpose

   encodes multiple copies of an epitope tag V5

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _V5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-GalT

   Plasmid

   #11929

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Promoter

   Available since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-CPDSalI

   Plasmid

   #38251

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL7.0: tgRFPt-SSPB WT

   Plasmid

   #60415

   Purpose

   Mammalian Expression of tgRFPt-Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   tgRFPt-SSPB
   

   Use

   Cre/Lox and Lentiviral

   Tags

   TagRFPt

   Expression

   Mammalian

   Mutation

   Y11K and A15E

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTKRED

   Plasmid

   #41062

   Depositor

   Thomas Kuhlman, Edward Cox

   Article

   Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM668

   Plasmid

   #68890

   Purpose

   pExchange(tdk) - B. thetaiotaomicron suicide vector expressing thymidine kinase

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Synapsin-pHoenix-WPRE

    Plasmid

    #70111

    Purpose

    lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    pHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
    

    Use

    Lentiviral

    Tags

    Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbS5a-Opto-Cre-Vvd-2

    Plasmid

    #160400

    Purpose

    Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    Opto-Cre-Vvd-2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJR103

    Plasmid

    #187242

    Purpose

    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAJM.657

    Plasmid

    #108525

    Purpose

    CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    CymRAM + PCymRC-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CymR: S110G A171V

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA Flag ERbeta

    Plasmid

    #35562

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQC membrane TdTomato IX

    Plasmid

    #37351

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    membrane TdTomato
    

    Use

    Tags

    Palmitoylation signal*

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-mCherry-IRES-Cre

    Plasmid

    #55632

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Inserts

    mCherry
    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSK9065

    Plasmid

    #84345

    Purpose

    tightly controlled expression of mRFP fusion proteins in S. aureus

    Depositor

    Neville Firth

    Article

    Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

    Type

    Empty backbone

    Use

    Tags

    mRFPmars

    Expression

    Bacterial

    Mutation

    Promoter

    Pxyl/tet

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-NRAS-Q61K

    Plasmid

    #116444

    Purpose

    Lentiviral expression of NRAS Q61K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Q61K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCP30T-E2Crimson

    Plasmid

    #78478

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    E2-Crimson
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-GfaABC1D-GRAB_ATP1.0

    Plasmid

    #167579

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEIGW roGFP2-Orp1

    Plasmid

    #64993

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTRE3G-Bi-α-chain

    Plasmid

    #133730

    Purpose

    Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    a-Chain_gePSI
    ZsGreen1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Only one part of the gePSI-system (aa1-141 + stop…

    Promoter

    pTRE3G-Bi

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. PB-CuO-V5 CASC3 siRes1+2 f.l. WT

    Plasmid

    #158540

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Orai1 CFP

    Plasmid

    #19757

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE PGK-GFP-IRES-LUC-W

    Plasmid

    #46793

    Purpose

    2nd generation lentiviral vector

    Depositor

    Darrell Kotton

    Article

    Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

    Inserts

    PGK
    eGFP (eGFP )
    IRES
    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. H2B-mGreenLantern

    Plasmid

    #164464

    Purpose

    Localization of mGreenLantern fluorescent protein to histone 2B

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Human Histone 2B (D26G and V119I mutations)

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pD454-SR mouse Alpha-synuclein

    Plasmid

    #89075

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMRE-Tn7-145

    Plasmid

    #118561

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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