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We narrowed to 18,759 results for: nls

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Showing: 7081 - 7120 of 18759 results

  2. pKM28

    Plasmid
    #134226
    Purpose
    Retroviral plasmid expressing EGFP - HJURP
    Depositor
    Iain Cheeseman
    Insert
    EGFP-HJURP-lacI (HJURP Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    134226-plasmid-map-sequence-id-271954

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  5. AAV-U6-gRNA-TnT-Cre

    Plasmid
    #132551
    Purpose
    For loss-of-function experiments in cardiomyocytes
    Depositor
    William Pu
    Inserts
    TnT-Cre
    U6-gRNA
    Use
    AAV
    Promoter
    TnT and U6
    Available since
    Oct. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132551-plasmid-map-sequence-id-258601

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  7. pAC1430

    Plasmid
    #127981
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127981-plasmid-map-sequence-id-254494

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  9. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Publication
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122510-plasmid-map-sequence-id-243592

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  15. pCAP03-ermE*p

    Plasmid
    #120234
    Purpose
    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Publication
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    Use
    Synthetic Biology
    Mutation
    bla (amp resistance gene)
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120234-plasmid-map-sequence-id-233748

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  17. pCAG-MCP-VPR-2AmScarlet

    Plasmid
    #121964
    Purpose
    Mammalian expression, Transcription regulation module
    Depositor
    Wei Li
    Publication
    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin
    Expression
    Mammalian
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    121964-plasmid-map-sequence-id-232191

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  23. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Publication
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101725-plasmid-map-sequence-id-199888

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  25. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92114-plasmid-map-sequence-id-197615

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  27. p330 VQR

    Plasmid
    #101730
    Purpose
    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101730-plasmid-map-sequence-id-192072

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  33. DNA-CARtcr-IRESpuro

    Plasmid
    #89346
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette
    Depositor
    Ron Vale
    Publication
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    Use
    Lentiviral
    Tags
    SNAPf tag
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89346-plasmid-map-sequence-id-176306

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  38. pLdCH

    Plasmid
    #84291
    Purpose
    Express gRNA and Cas9 in Leishmania with Hygromycin resistance
    Depositor
    Greg Matlashewski
    Publication
    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA and Cas9
    Use
    CRISPR; Leishmania
    Promoter
    L. donovani ribosome RNA promoter
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    84291-plasmid-map-sequence-id-170879

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Showing: 7081 - 7120 of 18759 results