164,304 results

1. pENTR221-BNT162b2ΔUTR

   Plasmid

   #171214

   Purpose

   Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

   Depositor

   Liang Wei Wang

   Article

   Low et al medRxiv 2021.04.27.21256151

   Insert

   BNT162b2ΔUTR
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Kozak sequence changed from CCCGCCACC to GGCTGCAC…

   Promoter

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWPI-TBX2

   Plasmid

   #185526

   Purpose

   Constitutive overexpression of human TBX2

   Depositor

   Eric Shusta, Sean Palecek

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   TBX2 (TBX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP1417 - pAAV-AiE2051m_3xC2-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #220650

   Purpose

   AiE2051m_3xC2 is an optimized enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

   Plasmid

   #181974

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; tNGFR (NGFR Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   NGFR truncation: aa 11-276

   Promoter

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP3653

   Plasmid

   #222348

   Purpose

   Expression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

   Depositor

   Jason Peters

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1-TRE-MA4-GFP

   Plasmid

   #114380

   Purpose

   Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

   Depositor

   Yuet Wai Kan

   Article

   Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   MLL-AF4 fusion gene (AFF1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-GCN5 core

   Plasmid

   #179551

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Linear-GFP

   Plasmid

   #226258

   Purpose

   Production of linear RNA via IVT

   Depositor

   Prashant Mali

   Article

   Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

   Insert

   EGFP
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-cfGFP

   Plasmid

   #200773

   Purpose

   Expresses cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   cysteine-free GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB-CMV-MCS-Puro cPLA2-EGFP

    Plasmid

    #162568

    Purpose

    Used as a measure of cPLA2 activation in live cells with an EGFP tag.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_53BP1_FLAG

    Plasmid

    #237345

    Purpose

    transient overexpression in mammalian cells

    Depositor

    Michele Pagano

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-MET

    Plasmid

    #116762

    Purpose

    Lentiviral expression of MET

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1-GfaABC1D-lck-jGCaMP8s

    Plasmid

    #176761

    Purpose

    AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Has Service

    AAV1 and AAV9

    Insert

    lck-jGCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-U6-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120392

    Purpose

    pAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Type

    Empty backbone

    Use

    AAV

    Tags

    mCherry.3XFLAG

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. scFv-GCN4-sfGFP-TET1CD

    Plasmid

    #184439

    Purpose

    TET1 catalytic domain fused with sfGFP and scFv against GCN4

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    scFv-GCN4-sfGFP-TET1CD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTL5

    Plasmid

    #127967

    Purpose

    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-phSyn-flex-SypEGFP

    Plasmid

    #153203

    Purpose

    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Lynette Lim

    Insert

    synaptophysin-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat synapsin

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-IRAlus-Nicn1

    Plasmid

    #92345

    Purpose

    Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

    Depositor

    Ling-Ling Chen, Gordon Carmichael

    Article

    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

    Insert

    Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mScarlet-PPM1H

    Plasmid

    #233585

    Purpose

    Expresses PPM1H in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-SIN-BX SpyCatcher003-hCD52

    Plasmid

    #210565

    Purpose

    Expresses Spycatcher003 on the cell surface using human CD52 hinge

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Spycatcher003 fused to human CD52 hinge
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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