8,806 results

1. EF.hHES1.Ubc.GFP

   Plasmid

   #17624

   Depositor

   Linzhao Cheng

   Article

   Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

   Insert

   HES1 (HES1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT_TO_DESTFLAGHA

   Plasmid

   #26361

   Depositor

   Thomas Tuschl

   Article

   Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

   Type

   Empty backbone

   Tags

   FLAG/HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. PaGFP-UtrCH

   Plasmid

   #26738

   Depositor

   William Bement

   Article

   Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

   Insert

   Utrophin
   

   Use

   Reporter for f-actin

   Tags

   photoactivatable GFP

   Expression

   Mammalian

   Mutation

   Calponin homology domain of utrophin (aa# 1-261)

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. His6-TEV-CRBN-midi

   Plasmid

   #215330

   Purpose

   CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

   Depositor

   Alessio Ciulli

   Article

   Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

   Insert

   CRBN (CRBN Human)
   

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 41-187 followed by a Gly-Ser…

   Promoter

   lacI

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 35S:RUBY

   Plasmid

   #160908

   Purpose

   RUBY under the control of CaMV 35S promoter/marker for transformation

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and CaMV 35S promoter
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-Tmem192-mRFP-3xHA

   Plasmid

   #134631

   Purpose

   bait for affinity-isolation of lysosomes

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3xHA, TEV, and mRFP

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiMPH v2

   Plasmid

   #89308

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Insert

   MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV-U6+27-Tornado-Broccoli

   Plasmid

   #124360

   Purpose

   Overexpression of circular RNA (Broccoli)

   Depositor

   Samie Jaffrey

   Article

   Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

   Insert

   Tornado-Broccoli
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-EGFP

   Plasmid

   #13031

   Purpose

   Mammalian expression vector for cloning your gene fused to EGFP

   Depositor

   Doug Golenbock

   Insert

   Enhanced Green Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+HyPer7

    Plasmid

    #136466

    Purpose

    Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Expression

    Bacterial

    Promoter

    native promoter

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Plenti-CMV-MCS-RFP-SV-puro

    Plasmid

    #109377

    Purpose

    This plasmid expresses dsRed2 and puromycin resistance

    Depositor

    Behzad Gerami-Naini, Jonathan Garlick

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmiRFP720-N1

    Plasmid

    #136560

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP720
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3 Basic Vector

    Plasmid

    #212936

    Purpose

    Firefly luciferase vector for investigating regions controlling transcription

    Depositor

    Debrya Groskreutz

    Type

    Empty backbone

    Use

    Luciferase

    Mutation

    WT

    Available Since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Lamp1-mScarlet-I

    Plasmid

    #98827

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lamp1
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2FA CO Tol2 TPase

    Plasmid

    #133032

    Purpose

    Expression vector for production of codon optimized Tol2 transposase

    Depositor

    Charles Nathan Hancock

    Article

    Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

    Insert

    Codon optimized Tol2 Transposase
    

    Use

    In vitro expression

    Mutation

    Codon Optimized

    Available Since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEcgRNA

    Plasmid

    #166581

    Purpose

    CRISPR-Cas9-assisted genome editing in Escherichia coli

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    ccdB
    

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiV_Cas9_puro

    Plasmid

    #108100

    Purpose

    Lentiviral expression plasmid of spCas9 with puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS promoter

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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