8,727 results

1. pCMV-DeAct-GS1

   Plasmid

   #89445

   Purpose

   Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-GS1 (GSN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning cytoplasmic Gsn amino acids 5…

   Promoter

   CMV

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Rc/CMV cyclin E

   Plasmid

   #8963

   Depositor

   Bob Weinberg

   Article

   Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

   Insert

   cyclin E (CCNE1 Human)
   

   Use

   Tags

   GPI linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLHKII-pGFPN3

   Plasmid

   #21920

   Depositor

   Hossein Ardehali

   Article

   Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

   Insert

   Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This is the full length human HKII cDNA sequence …

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmGFP

   Plasmid

   #117926

   Purpose

   Control plasmid expressing myc-GFP.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-i53

   Plasmid

   #92170

   Purpose

   recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

   Depositor

   Daniel Durocher

   Article

   Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

   Insert

   i53
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   UbvG08 with I44A mutation and no terminal Glycines

   Promoter

   CMV

   Available since

   July 13, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pCS2-MT GLI2 FL

   Plasmid

   #17648

   Depositor

   Erich Roessler

   Article

   Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

   Insert

   GLI2 (GLI2 Human)
   

   Use

   Tags

   6x Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Slug_pGL2

   Plasmid

   #31695

   Depositor

   Paul Wade

   Article

   Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

   Insert

   Slug (SNAI2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKCcas9dO

   Plasmid

   #62552

   Purpose

   High efficiency Streptomyces genome editing by CRISPR/Cas9 system

   Depositor

   Weihong Jiang

   Article

   Huang et al Acta Biochim Biophys Sin (Shanghai). 201

   Inserts

   Scocas9
   sgRNA ()
   act-orf4 homology arm
   

   Use

   CRISPR; Bacterial genome editing

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   j23119 and ptipA

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS mShh (CT#258)

   Plasmid

   #13999

   Depositor

   Cliff Tabin

   Insert

   Shh (Shh Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MuHKII-pGFPN3

    Plasmid

    #21922

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the full length human HKII cDNA sequence …

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

11. Hes5-Luc

    Plasmid

    #41724

    Depositor

    Raphael Kopan, Ryoichiro Kageyama

    Article

    Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

    Insert

    Hes5 Promoter (Hes5 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes5 promoter (-800 to +73)

    Promoter

    Hes5

    Available since

    Feb. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

12. FLAG-Pol-II WT

    Plasmid

    #35175

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Human Beta-catenin GFP

    Plasmid

    #71367

    Purpose

    mammalian expression of human Beta-catenin GFP

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    Beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTight-9-124-BclxL

    Plasmid

    #60857

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTight

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-FUSN-mCh-Cry2WT

    Plasmid

    #101223

    Purpose

    FUS(1-214) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHaloTag-EGFP

    Plasmid

    #86629

    Purpose

    Expresses HaloTag-eGFP tandem (cytosolic)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-PE7 for IVT

    Plasmid

    #214813

    Purpose

    Template for in vitro transcription of PE7

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge