We narrowed to 25,129 results for: crispr
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Plasmid#80784PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the arrayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-miRFP670
Plasmid#91854PurposeCas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988DepositorInsertshSpCas9-2A-miRFP670
BB-guide RNA
UseCRISPRTags2A-miRFP670 and 3XFLAGExpressionMammalianPromoterCbh and U6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMA-SpCas9-g2
Plasmid#80785PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the arrayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti dCAS-VP64_Blast
Plasmid#61425Purpose3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)DepositorHas serviceLentiviral PrepInsertdCAS9(D10A, N863A)-VP64_2A_Blast
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX-sgRNA
Plasmid#50662PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)DepositorInsertsgAAVS1
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MLM3636
Plasmid#43860Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoterDepositorInsertNone
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMA-SpCas9-g3
Plasmid#80786PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the arrayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g4
Plasmid#80787PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the arrayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
p458 VQR
Plasmid#101727PurposeExpresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 VQR
ExpressionMammalianMutationVQRAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX138
Plasmid#229213PurposeCRISPR array plasmid for NOTCH2 (Spacers 25-48)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR array plasmid for NOTCH2 (Spacers 25-48)
ExpressionMammalianAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A mNeonGreen P2A puro
Plasmid#122183PurposeThe plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXR001: EF1a-CasRx-2A-EGFP
Plasmid#109049PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targetingDepositorInsertCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianPromoterEF1AAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX333
Plasmid#64073PurposeVector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.DepositorTypeEmpty backboneTags3XFLAG-Cas9ExpressionMammalianPromoterCBh; U6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMZ379
Plasmid#74215PurposeCombined sgRNA/Cas9 vector with NatMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRExpressionBacterialPromoterpij23119Available sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
gRNA array
UseCRISPRPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ1658-dCas9-EGFP
Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianPromoterMSCV LTR promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by