159,132 results

1. pCAG-OSF-PP

   Plasmid

   #154247

   Purpose

   backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Type

   Empty backbone

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Use

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_SynP_DIO_tAKARα _WPRE

   Plasmid

   #196675

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   tAKARα
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD649-HAsp-SCN1B-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156534

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SCN1B (SCN1B Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-hXRCC1

   Plasmid

   #70759

   Purpose

   bacterial expression of human XRCC1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Tags

   6-HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lac operon

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. MLM3613

   Plasmid

   #42251

   Purpose

   Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIP_RNA1

   Plasmid

   #61259

   Purpose

   Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

   Depositor

   David Wang

   Article

   Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

   Insert

   Orsay RNA1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   heat shock promoter

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Cre

   Plasmid

   #198281

   Purpose

   Expresses Cre in mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJYS2_crtYf

   Plasmid

   #85544

   Purpose

   Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a-mEmerald-CAPRIN1

    Plasmid

    #164218

    Purpose

    Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    CAPRIN1 (CAPRIN1 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJMP3

    Plasmid

    #79875

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into thrC

    Depositor

    Jason Peters, Stanley Qi, Carol Gross

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHIP_RNA2FLAG

    Plasmid

    #61261

    Purpose

    Flag tagged Orsay virus RNA2 genome segment expression plasmid

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Use

    Tags

    Flag fused

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG148 FRB-NFAST

    Plasmid

    #130812

    Purpose

    Expresses FRB-NFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FRB-NFAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. UKNP1.23.1

    Plasmid

    #98206

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-C1-hAtg2A

    Plasmid

    #36456

    Depositor

    Noboru Mizushima

    Article

    Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

    Insert

    autophagy-related protein 2 homolog A (ATG2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Proline 656 to Arginine

    Promoter

    CMV

    Available since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS xenopus Islet1 (DM#219)

    Plasmid

    #15015

    Depositor

    Douglas Melton

    Article

    Kelly et al Dev Dyn. 2000 Aug . 218(4):615-27.

    Insert

    islet1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-CaMKIIa-hChR2(H134R)-EYFP

    Plasmid

    #26969

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Histidine 134 is mutated to Arginine

    Promoter

    CaMKIIa

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. UKNP1.20.1

    Plasmid

    #98195

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DsRed-rab7 DN

    Plasmid

    #12662

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mVenus-SiT-N-15

    Plasmid

    #56336

    Purpose

    Localization: Golgi TGN, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

21. pcDNA3-HA-VprBP

    Plasmid

    #133586

    Purpose

    Expresses human VprBP wildtype in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    Vpr (HIV-1) binding protein (DCAF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Wildtype human VprBP (Dcaf1)

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. Otx1/pBS

    Plasmid

    #16332

    Depositor

    Peter Klein

    Insert

    Otx1 (otx1 Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

23. KRphi-mRFP

    Plasmid

    #17276

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

    Insert

    KR phi
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pLenti-SFNp-mRFP1

    Plasmid

    #238473

    Purpose

    Expresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backbone

    Depositor

    Christopher Nelson

    Insert

    SFNp-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    SFNp

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLIX403 RNaseH1

    Plasmid

    #237695

    Purpose

    Inducible lentiviral expression of RNaseH1

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    RNaseH1 (RNASEH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MCUabaa-Flag-PLYS1

    Plasmid

    #236786

    Purpose

    MCU-MCUB fusion in abaa pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MCUabab-Flag-pLYS1

    Plasmid

    #236788

    Purpose

    MCU-MCUB fusion in abab pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lentiCRISPR v2-sgPnpt1-2#

    Plasmid

    #234770

    Purpose

    knockout Pnpt1

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Pnpt1 (Pnpt1 )
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AiP1788 - pAAV-AiE2116m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220713

    Purpose

    AiE2116m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AiP13776 - pAAV-AiE0050m_3xC2-minBG-iCre-WPRE3-BGHpA (Alias: CN3776)

    Plasmid

    #230380

    Purpose

    AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minRho

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAVtri-CMV-eGFP

    Plasmid

    #230932

    Purpose

    Used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 18065-M03-412

    Plasmid

    #225666

    Purpose

    Lentivirus protein expression. Control plasmid.

    Depositor

    Dominic Esposito

    Article

    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

    Inserts

    dsCopGFP
    delCD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    C-terminally truncated (aa 1-333 only)

    Promoter

    WPRE-SV40p and mCMVp

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMX-Flag-GFP_HOMER2

    Plasmid

    #227953

    Purpose

    Co-localisation experiment

    Depositor

    Alessandro Ori

    Insert

    HOMER2 (HOMER2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJBM3

    Plasmid

    #225290

    Purpose

    E. coli-Campylobacter shuttle vector; KanR

    Depositor

    Robert Moore

    Article

    McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

    Insert

    Replication elements from pCC388 for pJBM3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. XLone-Cas9-2A-GFP

    Plasmid

    #170159

    Purpose

    Express Cas9 and GFP upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    Cas9
    

    Use

    Synthetic Biology; Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G promoter

    Available since

    July 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CEL-Frb-EGFP-HOTag3

    Plasmid

    #208748

    Purpose

    part A of SparkDrop-Frb

    Depositor

    Xiaokun Shu

    Article

    Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

    Insert

    CEL-Frb-EGFP-HOTag3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. NTHL1-mCherry

    Plasmid

    #215145

    Purpose

    Expresses NTHL1-mCherry in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    NTHL1 (NTHL1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF2605

    Plasmid

    #144214

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYRF (MYRF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Perox-mTurq2-Apollo-NADP+

    Plasmid

    #193304

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2, peroxisomal targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge