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We narrowed to 748 results for: Sf1

Showing: 41 - 80 of 748 results

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LLP778_Traptavidin-p65HSF1-CO

Plasmid

#211771

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-p65HSF1

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP776_SpyCatcher-p65HSF1-CO

Plasmid

#211769

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-p65HSF1

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303/7A

Plasmid

#118365

Purpose

This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A and S307A

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-CSF1R

Plasmid

#23928

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSF1R (CSF1R Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra1

Plasmid

#184932

Purpose

Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1

Use

Tags

Expression

Worm

Mutation

Promoter

tag-168

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

Plasmid

#79372

Purpose

Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-p65-hsf1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSF1C-A-RFP

Plasmid

#172431

Purpose

StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Type

Empty backbone

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A DHFR.cHSF1

Plasmid

#134737

Purpose

Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

DHFR

Expression

Mutation

Deletion of amino acids 186-202

Promoter

None

Available since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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hHSF1 K298R

Plasmid

#118362

Purpose

This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K298R

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal

Plasmid

#197251

Purpose

Expresses the protein of wmCardinal in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-HTATSF1__BRS3

Plasmid

#116822

Purpose

Lentiviral expression of HTATSF1__BRS3 fusion

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

HTATSF1__BRS3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1aMS2p65HSF1hyg-W

Plasmid

#112920

Purpose

Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

MS2p65HSF1-T2Ahyg

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR1A dn-cHSF1

Plasmid

#134724

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

None

Available since

July 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Esf1

Plasmid

#99987

Purpose

Express yeast Esf1 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Use

Tags

Y2H binding domain

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-RASSF1C

Plasmid

#1977

Depositor

Joseph Avruch

Article

Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

Insert

RASSF1C (Rassf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pmiRGLO-TNFRSF10B

Plasmid

#117373

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR TNFRSF10B

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSuper-TatSF1 GH

Plasmid

#31120

Depositor

Mariano Garcia-Blanco

Article

Miller et al PLoS One. 2009 . 4(5):e5710.

Insert

TatSF1 shRNA (HTATSF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pSuper-TatSF1 AB

Plasmid

#31119

Depositor

Mariano Garcia-Blanco

Article

Miller et al PLoS One. 2009 . 4(5):e5710.

Insert

TatSF1 shRNA (HTATSF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pXTK024 [Origin_RSF1010_PartType1]

Plasmid

#229261

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Insert

RSF1010 Origin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSF11-wmNeonGreen

Plasmid

#197242

Purpose

Expresses the protein of wNeonGreen in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmNeonGreen

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP2

Plasmid

#178969

Purpose

Expresses miRFP2 in worm cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

wmiRFP2

Use

Tags

no

Expression

Worm

Mutation

NO

Promoter

tag-168 promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pST_B4YSF1_9LAMI (pBS0347)

Plasmid

#185186

Purpose

For the mammalian expression of the Dorcoceras hygrometricum protein B4YSF1_9LAMI. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

B4YSF1_9LAMI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hHSF1 S307A

Plasmid

#118364

Purpose

This plasmid expresses human HSF1 S307A mutant.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S307A

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2-Esf1

Plasmid

#99988

Purpose

Express yeast Esf1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Use

Tags

Y2H activation domain

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Ssf1

Plasmid

#63778

Purpose

yeast ORF Ssf1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Ssf1

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113343

Purpose

Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

p300core-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113341

Purpose

Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6/V5-DEST-ASF1B

Plasmid

#31205

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

ASF1B (ASF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
hHSF1 RNAi resistant

Plasmid

#118347

Purpose

This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

RNAi resistance mutations (does not change amino …

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6/V5-DEST-HSF1

Plasmid

#31210

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

HSF1 (HSF1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
MS2-P65-HSF1_GFP

Plasmid

#61423

Purpose

Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBb(RSF1010)1k-GFPuv

Plasmid

#106395

Purpose

IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-hHSF1 shRNA

Plasmid

#118345

Purpose

This plasmid expresses shRNA against hHSF1.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 shRNA (HSF1 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CSF1R-V5/HIS

Plasmid

#201984

Purpose

expression of human CSF1R receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

CSF1R receptor tyrosine kinase (CSF1R Human)

Use

Tags

V5, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-mHSF1-zeo

Plasmid

#17755

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

HSF1 (Hsf1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pClneoFH human RASSF1A

Plasmid

#37016

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

rassf1A (RASSF1 Human)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL0_3 [RSF1010 ORI]

Plasmid

#198920

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSF107mT-GATEWAY-3'-FLAG

Plasmid

#67619

Purpose

A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Flag

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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