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We narrowed to 6,995 results for: tet

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Showing: 951 - 1000 of 6995 results

  1. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Promoter
    pTRE bidirectional tet-on
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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  4. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid
    #190236
    Purpose
    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase
    Depositor
    Hiroyuki Hioki
    Article
    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi
    Insert
    EGFP
    Use
    AAV
    Tags
    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…
    Expression
    Mammalian
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    190236-plasmid-map-sequence-id-381571

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  7. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid
    #24419
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    RS1 (HTR4 Human)
    tTA
    Use
    Mouse Targeting
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    EF1α and TRE, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24419-plasmid-map-sequence-id-11416

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  9. pBItetDMPKSGFP

    Plasmid
    #96903
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Expression
    Mammalian
    Promoter
    tetracycline responsive and bidirectional
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96903-plasmid-map-sequence-id-495754

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  13. pEnt R3L2 TetO(sh) mCh-Rs1-2

    Plasmid
    #24417
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Insert
    Rs1
    Use
    Gateway Cloning
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    short TetO, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24417-plasmid-map-sequence-id-11414

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  14. P20_992-tetO-deGFP

    Plasmid
    #97096
    Purpose
    Expresses GFP in the presence of Pseudomonas fluorescence sigma factor ECF20_992. Repressed by TetR
    Depositor
    Richard Murray
    Insert
    deGFP
    Expression
    Bacterial
    Promoter
    P20_992 with TetO operator site
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    97096-plasmid-map-sequence-id-195303

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  19. pLKO-Tet-puro-hRAF1-shRNA-1

    Plasmid
    #185371
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1
    Depositor
    Ayaz Najafov
    Insert
    RAF1 (RAF1 Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185371-plasmid-map-sequence-id-363455

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  31. pJFA344C7

    Plasmid
    #49236
    Purpose
    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    TALE-TET1CD Backbone (TET1 Human, Synthetic)
    Use
    TALEN; Tale-tet1 expression vector
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    49236-plasmid-map-sequence-id-72802

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  38. pBItetDT12nGFP

    Plasmid
    #80420
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80420-plasmid-map-sequence-id-151410

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  40. pTet-O-APOE-E4-T2A-Puro

    Plasmid
    #190813
    Purpose
    Express APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Mutation
    E4
    Promoter
    Tet-O
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190813-plasmid-map-sequence-id-372861

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  46. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid
    #185370
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185370-plasmid-map-sequence-id-363837

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  47. pBItetDT480GFP

    Plasmid
    #96905
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Expression
    Mammalian
    Promoter
    tetracycline responsive and bidirectional
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    96905-plasmid-map-sequence-id-306112

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  48. pBItetDT240GFP

    Plasmid
    #96904
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Expression
    Mammalian
    Promoter
    tetracycline responsive and bidirectional
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96904-plasmid-map-sequence-id-193222

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  50. QUAS-0-T7-TetO-GFP_T7-LacO-TetR_ColE1

    Plasmid
    #171663
    Purpose
    QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QUAS-T7-TetO-GFP ssrA-T7 Stop- T7-LacO-TetR-T7 Stop
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171663-plasmid-map-sequence-id-339028

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Showing: 951 - 1000 of 6995 results