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We narrowed to 44,104 results for: cha

Showing: 10301 - 10350 of 44104 results

  1. AAV-GfaABC1D-lck-smV5-4x6T-WPRE

    Plasmid
    #196416
    Purpose
    Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smV5
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and GfaABC1D
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196416-plasmid-map-sequence-id-385161

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  4. AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE

    Plasmid
    #196414
    Purpose
    Astrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smFLAG
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and GfaABC1D
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196414-plasmid-map-sequence-id-385111

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  7. FUT8-Dox

    Plasmid
    #124631
    Purpose
    Doxycycline-inducible FUT8 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Alpha-(1,6)-fucosyltransferase
    Tetracycline repressor A3 mutant
    TagFP635
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight and hEF1a
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124631-plasmid-map-sequence-id-248079

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  14. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid
    #67275
    Purpose
    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do
    Insert
    puromycin resistant gene
    Use
    Tags
    linked to red flouresent protein gene (katushka)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67275-plasmid-map-sequence-id-118496

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  23. pET28-Mff(1-61)-PP-GST

    Plasmid
    #73042
    Purpose
    Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site
    Depositor
    David Chan
    Article
    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
    Insert
    Mff(1-61) (Mff Mouse)
    Use
    Tags
    GST-tag and PreScission protease site
    Expression
    Bacterial
    Mutation
    Truncation containing amino acids 1-61
    Promoter
    T7
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73042-plasmid-map-sequence-id-134011

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  25. pET21-Drp1

    Plasmid
    #72927
    Purpose
    Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site
    Depositor
    David Chan
    Article
    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
    Insert
    Drp1 (Dnm1l Mouse)
    Use
    Tags
    6xHis-tag and PreScission Protease site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72927-plasmid-map-sequence-id-137706

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  29. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid
    #101300
    Purpose
    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.
    Depositor
    Alpha Yap
    Article
    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.
    Insert
    alpha-Catenin
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains amino acids 1-509, 670-698, and 699-906 …
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    101300-plasmid-map-sequence-id-246103

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  34. p-mCherry-C1-iChR2++

    Plasmid
    #98172
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iChR2++ gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98172-plasmid-map-sequence-id-182770

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  38. pcDNA3-LYRa11-RBD-8h

    Plasmid
    #161822
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T324-D522)
    Promoter
    CMV
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161822-plasmid-map-sequence-id-316758

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  39. pcDNA3-SARS-1-RBD-8h

    Plasmid
    #161821
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T320-D518)
    Promoter
    CMV
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161821-plasmid-map-sequence-id-316749

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  41. pcDNA3-RsSHC014-RBD-8h

    Plasmid
    #161826
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161826-plasmid-map-sequence-id-316777

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  43. pICE-HA-CtIP-siR-WT

    Plasmid
    #82030
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Mutations making the cDNA resistant to a siRNA
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82030-plasmid-map-sequence-id-155378

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  45. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid
    #129394
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV and Cre/Lox
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129394-plasmid-map-sequence-id-259728

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  46. pcDNA3-Rs7327-RBD-8h

    Plasmid
    #161823
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161823-plasmid-map-sequence-id-316772

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Showing: 10301 - 10350 of 44104 results