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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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179,521 results

Showing: 8361 - 8400 of 179521 results

  3. CAG pShuttle

    Plasmid
    #13427
    Depositor
    Ronald Kahn
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available since
    Nov. 1, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13427-plasmid-map-sequence-id-258367

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  6. pLVU-tTR-KRAB

    Plasmid
    #11645
    Purpose
    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation
    Depositor
    Patrick Aebischer, Didier Trono
    Publication
    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:
    Insert
    hUbiquitin C, GFP, tTR-KRAB, no tetO
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11645-plasmid-map-sequence-id-6991

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  12. 803 pGEX2T PTEN

    Plasmid
    #10784
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    GST
    Expression
    Bacterial
    Available since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  18. pCMV-PE6f

    Plasmid
    #207856
    Purpose
    Mammalian expression of PE6f prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6f
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207856-plasmid-map-sequence-id-408168

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  19. pCMV-PE6g

    Plasmid
    #207857
    Purpose
    Mammalian expression of PE6g prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6g
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207857-plasmid-map-sequence-id-408164

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  26. pYPQ-dzCas9-Act3.0

    Plasmid
    #158414
    Purpose
    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158414-plasmid-map-sequence-id-332091

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  30. pETM33_Nsp5_Mpro

    Plasmid
    #156475
    Purpose
    Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Publication
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    156475-plasmid-map-sequence-id-307727

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  37. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Publication
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128095-plasmid-map-sequence-id-249667

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  38. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Publication
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128096-plasmid-map-sequence-id-249669

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Showing: 8361 - 8400 of 179521 results