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172,006 results

Showing: 10501 - 10550 of 172006 results

  4. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  5. HaloTag-A2A-adenosine receptor

    Plasmid
    #66995
    Purpose
    A2A-adenosine receptor tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    Tags
    Human Growth hormone Signal Sequence- HaloTag
    Expression
    Mammalian
    Mutation
    The signal sequence from human growth hormone was…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66995-plasmid-map-sequence-id-115422

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  6. eGFP-ova-C1

    Plasmid
    #163524
    Purpose
    Expresses both eGFP and chicken ovalbumin in mammalian cells
    Depositor
    Thomas Weber
    Insert
    enhanced-GFP-chicken ovalbumin tandem
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163524-plasmid-map-sequence-id-321577

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  12. pMS82

    Plasmid
    #68110
    Purpose
    Integration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replication
    Depositor
    Maggie Smith
    Article
    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.
    Inserts
    aac3(IV)
    Hyg
    oriT
    attP
    int
    Use
    Streptomyces integrating vector
    Mutation
    Fragment
    Available Since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    68110-plasmid-map-sequence-id-248992

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  23. AKT3-NanoLuc Fusion Vector

    Plasmid
    #237208
    Purpose
    Express AKT3-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    AKT3 (AKT3 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237208-plasmid-map-sequence-id-476608

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  25. pAAV MP-84 mRFP

    Plasmid
    #206926
    Purpose
    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.
    Depositor
    Markus Grompe
    Article
    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2
    Insert
    mRFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206926-plasmid-map-sequence-id-407770

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  34. pOCC175

    Plasmid
    #118890
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; mGFP, cleav…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118890-plasmid-map-sequence-id-232288

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  37. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  40. DNMT1

    Plasmid
    #25165
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    dnmt1.0351.0600 (DNMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  42. pEGFP-C1-BBS1

    Plasmid
    #218711
    Purpose
    Expresses N-terminally EGFP-tagged BBS1 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    BBS1 (BBS1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218711-plasmid-map-sequence-id-434142

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  43. Halo-53BP1 HRD

    Plasmid
    #207087
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207087-plasmid-map-sequence-id-431238

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Showing: 10501 - 10550 of 172006 results