We narrowed to 82,273 results for: TRI
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Plasmid#60238PurposeHTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intronDepositorInsertinmCherry
UseRetroviralAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct(delta1-6)-mRuby3
Plasmid#155223PurposeF-actin bidning mutant of MPActDepositorInsertMutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
TagsmRuby3ExpressionMammalianMutationDeletion of first 6 AA from F-tractin (9-14 from …Available SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR-NES
Plasmid#118407PurposeCytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR-NES
TagsNuclear export signal (NES)ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP5 (catalytic domain)
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
TagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s
Plasmid#100022PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.DepositorInsertsfGCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA MOV10 K530A
Plasmid#51716Purposeplasmid expresses FLAG/HA-tagged MOV10 K530ADepositorAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA MOV10 D645N
Plasmid#51717Purposeplasmid expresses FLAG/HA-tagged MOV10 D645NDepositorAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-Cas9n-UGI
Plasmid#119135PurposeBase editor made from full-length APOBEC3B with an L1 intron to prevent self-restrictionDepositorInsertAPOBEC3B L1 intron-Cas9 nickase-UGI
UseCRISPRMutationL1 intron insertionPromoterCMVAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM09
Plasmid#135482PurposeAgrobacterium T-DNA with SpunHsp70pr driving hph-GFPDepositorInsertSpunHsp70pr hph-GFP ADH1t
TagsGFPExpressionBacterial and YeastPromoterSpunHsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B3
Plasmid#172844PurposeCRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mTurquoise2
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(YF)-mCherry
Plasmid#87357PurposePI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutationDepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationY535FPromoterCMVAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-Arp11 (1-417)
Plasmid#51398Purposeexpression of full-length mouse Arp11 in mammalian cellsDepositorAvailable SinceMarch 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1079A
Plasmid#83494PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)DepositorInsertLbu_C2c2_R1079A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1079APromoterT7Available SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B4
Plasmid#172845PurposeCRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mNeonGreen
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD_Psra-6::ChR2(H134R)::mCherry
Plasmid#117419PurposeFor selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoterDepositorInsertchannel rhodopsin-2 with H134R mutation
ExpressionWormMutationH134RPromotersra-6Available SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd8-1D4
Plasmid#42270DepositorAvailable SinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SuperNovaGreen-T2A-dTomato
Plasmid#193798PurposeExpresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomatoDepositorInsertSuperNova Green
UseAAVTagsFlag tagExpressionMammalianPromoterCAGAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 MCV LTco S239A
Plasmid#112194PurposeExpression of MCPyV LT mutant (Ser 239 to Ala)DepositorInsertMCPyV LT codon optimized
ExpressionMammalianPromoterCMVAvailable SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-PD-1
Plasmid#223572PurposeLentiviral expression of human PD-1 with SNAP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
TagsGFP and hphExpressionBacterial and YeastPromoterSpun Hsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-miR-34 MT
Plasmid#78259PurposepsiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated miR-34 target site
UseLuciferase; Microrna activityExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CCGT to GG…Available SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDest Lenti CA-GFP
Plasmid#197459PurposeBicistronic expression vector expressing CA-GFP under the CMV promotor separated by an internal ribosome entry site (IRES) mediated expression of dTomato reporter protein.DepositorInsertGFP
UseLentiviralExpressionMammalianMutationS65TPromoterCMV, IRESAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A
Plasmid#83484PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).DepositorInsertLbu_C2c2_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1048A, H1053AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(KD)-mCherry
Plasmid#87358PurposePI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutationDepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationD388RPromoterCMVAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(WT)
Plasmid#86561PurposeExpresses human full-length Akt1(WT)DepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd2-1D4
Plasmid#42264DepositorAvailable SinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd4-1D4
Plasmid#42266DepositorAvailable SinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Xyl-DocI
Plasmid#58711PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertXyl-DocI
TagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Threonin 120 to Cysteine in Xylanase Doma…PromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pZ005 (AacC2c1 human codon optimized protein)
Plasmid#70171PurposeExpresses AacC2c1 human codon optimized proteinDepositorInsertAacC2c1 protein
TagsNLSAvailable SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-CXXCmut-pEF
Plasmid#127895PurposeExpresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAADepositorAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd9-1D4
Plasmid#42271DepositorAvailable SinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
645 pCS2-etv2
Plasmid#49005PurposeMammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2DepositorInsertets variant gene 2 (etsrp Zebrafish)
UseInvitro transcription, riboprobe templateExpressionMammalianAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
Bongwoori
Plasmid#63720PurposeConsists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227DDepositorInsertBongwoori
TagsSuper ecliptic pHlorin A227DExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME DN-E-cadherin
Plasmid#135209PurposeDominate Negative zebrafish e-cadherin labelDepositorInsertCDH1
UseSynthetic BiologyAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458_TFE3_2
Plasmid#86326PurposeEncodes gRNA for 3' target of human TFE3DepositorInsertgRNA against TFE3 (TFE3 Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKH143_EGFP-YIPF3 LIR mut
Plasmid#214975PurposeGFP tagged LIR mutant YIPF3DepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A
Plasmid#83483PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pInsert-PhoCl-RER
Plasmid#219676PurposeCaging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2DepositorInsertCD4mScarlet-PhoCl-RER
TagsmScarlet and PhoClExpressionMammalianPromoterTREAvailable SinceJune 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFKBPmCherry-Akt2(K14A)
Plasmid#86597PurposeExpresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutantDepositorInsertFKBB-mCherry-Akt2(K14A) (AKT2 )
ExpressionMammalianAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y
Plasmid#148087PurposeInsect Expression of DmPAN3-W707AG711E-dsRNAresDepositorInsertDmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PML-ER-pBabe
Plasmid#73556Purposevector encoding a fusion protein between PML-IV and ER (estrogen receptor)DepositorAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)
Plasmid#86595PurposeExpresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutantDepositorInsertAkt2(K14A/R25E) (AKT2 )
ExpressionMammalianAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKH163_pSmart-mNEON-YIPF4
Plasmid#214982Purposeendogenous mneon tag for YIPF4DepositorInsertmNEON with YIPF4 homology (YIPF4 Human)
UseCRISPRAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP3(307-574)
Plasmid#71481PurposeEncodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 3 (SENP3 Human)
TagsHexahistidineExpressionBacterialMutationDeleted amino acids 1-306 (catalytic domain only)PromoterT7Available SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-VSVG-16
Plasmid#57693PurposeLocalization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVSVG
TagstdEosExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJet-CMV-CD5Flag2-bglpA
Plasmid#89705PurposeExpression plasmid for Flag embedded into human CD52 with short polyADepositorInsertCMV-CD5Flag2-bglpA
ExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only