159,157 results

1. p-cyclinB1-mcherry (1958)

   Plasmid

   #26063

   Depositor

   Jonathon Pines

   Article

   Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. Talin1 head EGFP fusion

   Plasmid

   #221408

   Purpose

   Mammalian expression of Talin1 head EGFP fusion for mammalian expression

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   Talin1 head EGFP fusion (TLN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHCD1C

   Plasmid

   #102954

   Purpose

   gene cloning of human CD1C

   Depositor

   Lucia Mori, Gennaro De Libero

   Article

   Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

   Insert

   CD1C cDNA (CD1C Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-3xFLAG-NLS-SpCas9-NLS

   Plasmid

   #51307

   Purpose

   Cas9 expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Insert

   3xFLAG-NLS-SpCas9-NLS
   

   Use

   CRISPR; Xenopus tropicalis expression

   Tags

   3x Flag and NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMIG-Aalpha WT

   Plasmid

   #10884

   Depositor

   William Hahn

   Article

   Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

   Insert

   PP2A Aalpha (PPP2R1A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABI_cTEVp

   Plasmid

   #119214

   Purpose

   Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cTEVp in fusion with ABI
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TPH2-Cre

   Plasmid

   #189616

   Purpose

   Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Cre Recombinase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tph2

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207860

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. VPS13A^Halo

    Plasmid

    #118759

    Purpose

    VPS13A internally tagged with Halo at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Use

    Tags

    Halo tag inserted at position 1372

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEVA3b67Rb

    Plasmid

    #58311

    Purpose

    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-puro-EWS-FLI1

    Plasmid

    #102813

    Purpose

    Overexpress EWS-FLI1. 3rd generation lentiviral vector.

    Depositor

    Jialiang Wang

    Article

    Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

    Insert

    EWS-FLI1 (EWSR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-T/O-Ds1-mCherry

    Plasmid

    #112855

    Purpose

    Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein

    Depositor

    David Sprinzak

    Article

    Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

    Insert

    DCHS1 (DCHS1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline inducible promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LCV2 IRF3 KO

    Plasmid

    #217446

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    sgRNA targeting IRF3 (IRF3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. medSIIN

    Plasmid

    #185664

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG1345 mCherry-FIREtag-DEVDG-MoA

    Plasmid

    #205176

    Purpose

    Expresses mCherry-FIREtag-DEVDG-MoA in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-cagedFIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-HDAC1-Hygro

    Plasmid

    #104409

    Purpose

    transient expression of dCas9-HDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. GST-TAX1BP1

    Plasmid

    #171423

    Purpose

    For the purifcation of TAX1BP1 protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Luc-T20

    Plasmid

    #177941

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pB-mTSPAN4-GFP

    Plasmid

    #210329

    Purpose

    Overexpression of mTSPAN4

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mTSPAN4 (Tspan4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PLIN2-V5-APEX2

    Plasmid

    #170573

    Purpose

    A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    PLIN2 (Plin2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Ube1/PET21d

    Plasmid

    #34965

    Depositor

    Cynthia Wolberger

    Article

    Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

    Insert

    Ube1 (UBA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA Flag Yap1

    Plasmid

    #18881

    Depositor

    Yosef Shaul

    Article

    Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

    Insert

    Yap1 (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTOPO-MCL1

    Plasmid

    #21605

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 (MCL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert ends with a STOP codon, no V5-His-tag.

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pUC19-mAirn-R-loop

    Plasmid

    #134900

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mAirn promoter (Airn Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV_SynP_DIO_tAKARα _WPRE

    Plasmid

    #196675

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    tAKARα
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SELP-bio-His

    Plasmid

    #51639

    Purpose

    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SELP (SELP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EF1a-EGFP (no signal control)

    Plasmid

    #170167

    Purpose

    Mammalian Expression of no signal control

    Depositor

    Christopher Moxham

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAVS1_Puro_hPGK1_EBFP_Donor

    Plasmid

    #178089

    Purpose

    HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EBFP (T65S/F145Y)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Fragment1 with TLS2

    Plasmid

    #196983

    Purpose

    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Intermediate fragment for assembly of gRNA-TLS2 construct
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CAG FLEX BARK D110A p2A mCherry

    Plasmid

    #117694

    Purpose

    CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs D110A peptide
    

    Use

    Cre/Lox

    Tags

    HA / FLAG

    Expression

    Mutation

    D110A

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGP-CMV-GCaMP6m

    Plasmid

    #40754

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6m
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EGFP-Rab4a-7

    Plasmid

    #56440

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. MAC-tag-N

    Plasmid

    #108078

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/BioID

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CCDB (ccdB(letD) )
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGST2-GST-TEV-OPTN

    Plasmid

    #198428

    Purpose

    Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUC18T-mini-Tn7T-Tp

    Plasmid

    #65024

    Purpose

    TpR mini-Tn7 vector, mobilizable

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

    Plasmid

    #112925

    Purpose

    Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6 promoter, gRNA-SAM expression cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge