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179,521 results

Showing: 8521 - 8560 of 179521 results

  8. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138272-plasmid-map-sequence-id-273617

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  11. pORANGE Tubb3-GFP KI

    Plasmid
    #131497
    Purpose
    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)
    Depositor
    Harold MacGillavry
    Publication
    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    Expression
    Mammalian
    Promoter
    U6 and Cbh
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131497-plasmid-map-sequence-id-257260

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  20. pHR-BRD4ΔN-mCherry-sspB

    Plasmid
    #121968
    Purpose
    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.
    Depositor
    Clifford Brangwynne
    Publication
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    BRD4 (BRD4 Human)
    Use
    Lentiviral
    Tags
    mCherry-sspB
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1-461
    Promoter
    SFFV
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    121968-plasmid-map-sequence-id-237552

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  28. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EJ7-GFP variant of EGFP
    Expression
    Mammalian
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113617-plasmid-map-sequence-id-218254

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  34. pAAV.GfaABC1D.PI.Cre.SV40

    Plasmid
    #105603
    Purpose
    AAV expression of Cre from GfaABC1D promoter
    Depositor
    Baljit Khakh
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105603-plasmid-map-sequence-id-378896

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  36. pSC101_TIMER

    Plasmid
    #103057
    Purpose
    low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
    Depositor
    Dirk Bumann
    Publication
    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1
    Insert
    TIMER-bac
    Expression
    Bacterial
    Mutation
    mutations: S4T and S179T
    Promoter
    constitutive ybaJp promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103057-plasmid-map-sequence-id-199056

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  37. DNMT3A-dCas9

    Plasmid
    #100090
    Purpose
    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A (DNMT3A Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-DNMT3A-dCas9-NLS
    Expression
    Mammalian
    Mutation
    includes aa 602-912
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100090-plasmid-map-sequence-id-197562

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  38. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

    Plasmid
    #101064
    Purpose
    AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
    Depositor
    Eric Schreiter
    Publication
    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:
    Has service
    AAV1
    Insert
    CaMPARI2_L398T
    Use
    AAV
    Tags
    FLAG-HA-myc and NES_his
    Promoter
    hsyn (synapsin-1)
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101064-plasmid-map-sequence-id-194371

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Showing: 8521 - 8560 of 179521 results