161,439 results

1. pGRNA2

   Plasmid

   #169779

   Purpose

   Insert one gRNA

   Depositor

   Sang-Gyu Kim

   Article

   Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SOST/pcDNA3.1+

   Plasmid

   #10842

   Depositor

   Xi He

   Article

   Semenov et al J Biol Chem. 2005 Jul 22. 280(29):26770-

   Insert

   SOST (SOST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 240 pTol2005b

   Plasmid

   #73483

   Purpose

   Tol2 vector with multi cloning site

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Type

   Empty backbone

   Use

   Tol2

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTagRFP657-N1

   Plasmid

   #31959

   Depositor

   Vladislav Verkhusha

   Article

   Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-SpyTagMBP

   Plasmid

   #35050

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyTagMBP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Simple peptide tag added to the N-terminus of MBP…

   Promoter

   T7 promoter

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

   Plasmid

   #175574

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBlueSKMimRNA

   Plasmid

   #102535

   Purpose

   Template for synthesis of Minos transposase mRNA

   Depositor

   Michalis Averof

   Article

   Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

   Insert

   Minos transposase mRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

   Plasmid

   #44069

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   12xHis tag, 6xGly linker, ADH1 transcription term…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-C1-AR Q48

   Plasmid

   #86428

   Purpose

   Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   Androgen receptor (AR) (AR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Poly-glutamine expansion, G130D

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPR101-Tango

    Plasmid

    #66301

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR101 (GPR101 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. PIK3C2GA

    Plasmid

    #42451

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PIK3C2GA (PIK3C2G Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCW empty vector

    Plasmid

    #184708

    Purpose

    Lentiviral empty vector for doxycycline-inducible expression

    Depositor

    Alessia Ciarrocchi, Gloria Manzotti

    Article

    Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNGFP-BC

    Plasmid

    #161956

    Purpose

    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

    Depositor

    Eric Gouaux

    Article

    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

    Type

    Empty backbone

    Use

    Tags

    GFPuv His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCGFP-BC

    Plasmid

    #161959

    Purpose

    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

    Depositor

    Eric Gouaux

    Article

    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

    Type

    Empty backbone

    Use

    Tags

    GFPuv His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC-Dest-BsaI

    Plasmid

    #200550

    Purpose

    Mini circle destination vector with BsaI sites for Golden Gate Assembly

    Depositor

    Junsu Kang

    Type

    Empty backbone

    Use

    Minicircle cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHsa.NFKB:d2mRFP1

    Plasmid

    #239991

    Purpose

    Destabilized red fluorescent protein (d2mRFP1) under transcriptional control of NF-kB activation

    Depositor

    Raquel Espin-Palazon

    Inserts

    mRFP1
    PEST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cfos minimal promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-mito-GreenPy1Highest

    Plasmid

    #239069

    Purpose

    Mammalian expression of mitochondria-targeted highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-GreenPy1Highest

    Plasmid

    #239059

    Purpose

    Mammalian expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-mito-ApplePy1Highest

    Plasmid

    #239074

    Purpose

    Mammalian expression of mitochondria-targeted highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    ApplePy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMB-Tl-ADPHK

    Plasmid

    #237469

    Purpose

    Expression of His-tagged Thermococcus litoralis ADP hexokinase

    Depositor

    Michael Baker

    Insert

    ADP hexokinase
    

    Use

    Tags

    C-terminal 10 His-tag and N-terminal strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDH-3_Flag-YTHDF2-m6A-MUT

    Plasmid

    #238976

    Purpose

    Expresses the m6A-binding-deficient mutant of YTHDF2

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2-m6A-MUT (YTHDF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    2 site mutation (W432A+W486A)

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETCK3 TGP-E

    Plasmid

    #236255

    Purpose

    Expresses TGP-E, a thermostable fluorescent protein

    Depositor

    Natasha DeVore

    Article

    Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

    Insert

    Thermal Green Protein Q66E (TGP-E)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac operon

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FLAG-FBXL17

    Plasmid

    #236188

    Purpose

    Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FLAG-FBXL17 (FBXL17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

    Plasmid

    #234883

    Purpose

    non-targeting CRISPRi control

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-hCGN

    Plasmid

    #236507

    Purpose

    For expression of Cingulin (human) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGN (CGN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXs-mAscl1

    Plasmid

    #233152

    Purpose

    Retroviral expression of Ascl1

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Flag-ZDHHC13

    Plasmid

    #231661

    Purpose

    Expresses human ZDHHC13 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC13 (ZDHHC13 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAIP4_2WWE

    Plasmid

    #234210

    Purpose

    Heterologous protein expression of PI3K-C2-gamma in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2WWE (PIK3C2G Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pSems StayGold-2xFYVE

    Plasmid

    #222948

    Purpose

    Expression of HRS 2xFYVE tagged with StayGold in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    StayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

    Plasmid

    #228363

    Purpose

    mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPB-cT3G-cERP2-EZH2

    Plasmid

    #222528

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. sgLATS1/2-2

    Plasmid

    #229432

    Purpose

    knockout of LATS1 and LATS2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgLATS1/2-2 (LATS1, LATS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

    Plasmid

    #184556

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

    Plasmid

    #217713

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA3 (ADGRA3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-743

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcdna 3.1 DSG-3 Tailless Control

    Plasmid

    #182718

    Purpose

    expresses a truncated force-insensitive DSG-3 TSmod

    Depositor

    Daniel Conway

    Article

    Jin et al iScience. 2025 Jul 9;28(8):113081. doi:

    Insert

    desmoglein-3 (Dsg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TSmod inserted at G726, downstream C-terminal tai…

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCDNA5 TEX264wt-cGFP

    Plasmid

    #220211

    Purpose

    Mammalian expression of wild type TEX264 fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

    Plasmid

    #217712

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA2 (ADGRA2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-752

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG

    Plasmid

    #217711

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA1 (ADGRA1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-6

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

    Plasmid

    #204811

    Purpose

    Bacterial expresison of Enterovirus A71 2A protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    A71 2A protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pHYX137

    Plasmid

    #202814

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScPCK1

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge